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Table 1.

Nrx-IV and wrapper MG phenotypes

Phenotypic classes
Genotype% Defective segments% Dissociated% Incomplete% Absent
Single mutant
 

 

 

 

 
Nrx-IV4304/TM3 3 (5/147) 40 (2/5) 0 (0/5) 60 (3/5) 
Nrx-IV4304/Nrx-IV4304 95 (168/177) 68 (114/168) 23 (39/168) 9 (15/168) 
wrapper175/+ 2 (4/185) 50 (2/4) 50 (2/4) 0 (0/4) 
wrapper175/wrapper175
 
99 (137/139)
 
76 (104/137)
 
24 (33/137)
 
0 (0/137)
 
Double mutant
 

 

 

 

 
wrapper175/+; Nrx-IV4304/+ 2 (1/52) 0 (0/1) 100 (1/1) 0 (0/1) 
wrapper175/wrapper175; Nrx-IV4304/Nrx-IV4304 96 (71/74) 30 (21/71) 68 (48/71) 3 (2/71) 
Phenotypic classes
Genotype% Defective segments% Dissociated% Incomplete% Absent
Single mutant
 

 

 

 

 
Nrx-IV4304/TM3 3 (5/147) 40 (2/5) 0 (0/5) 60 (3/5) 
Nrx-IV4304/Nrx-IV4304 95 (168/177) 68 (114/168) 23 (39/168) 9 (15/168) 
wrapper175/+ 2 (4/185) 50 (2/4) 50 (2/4) 0 (0/4) 
wrapper175/wrapper175
 
99 (137/139)
 
76 (104/137)
 
24 (33/137)
 
0 (0/137)
 
Double mutant
 

 

 

 

 
wrapper175/+; Nrx-IV4304/+ 2 (1/52) 0 (0/1) 100 (1/1) 0 (0/1) 
wrapper175/wrapper175; Nrx-IV4304/Nrx-IV4304 96 (71/74) 30 (21/71) 68 (48/71) 3 (2/71) 

Values in parentheses indicate the number of affected segments/total number of segments examined. For single mutants, segments A1-A7 were examined at stage 17 for defects in AMG migration using sim-Gal4 UAS-tau-GFP and anti-GFP to visualize all midline cells. AMG were identified based on morphology and position and the presence of Runt or Wrapper immunostaining. At stage 17, three phenotypes were observed: (1) Dissociated - dissociation of AMG from MP1 neurons and the failure of AMG to ensheath the PC; (2) Incomplete- dissociation coupled with a failure of AMG to ensheath the AC or PC (more severe than Dissociated alone); (3) Absent - complete absence of AMG. For double mutants, AMG were identified based on morphology, position and the presence of staining for the glial markers argos or Ect3. Homozygous Nrx-IV mutants were identified by the absence of Nrx-IV staining from the epidermis.

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