MSL complex components
| Component . | Protein/RNA type . | Mutant phenotype . | Proposed basis for chromosomal phenotype . | References . |
|---|---|---|---|---|
| MSL1 | Scaffold for MSL complex assembly | Male lethality; no X chromosome recognition | MSL1 and MSL2 are required together for CES recognition; MSL1 is unstable in the absence of MSL2 | (Palmer et al., 1994; Lyman et al., 1997; Chang and Kuroda, 1998; Scott et al., 2000; Morales et al., 2004) |
| MSL2 | RING finger protein | MSL2 is the limiting MSL component in males | (Palmer et al., 1994; Kelley et al., 1995; Lyman et al., 1997) | |
| MSL3 | Chromodomain protein | Chromodomain required for spreading | (Palmer et al., 1994; Buscaino et al., 2006; Sural et al., 2008) | |
| MOF | H4K16 acetyltransferase | Male lethality; partial MSL complex at CES | Histone acetyltransferase activity required for spreading | (Hilfiker et al., 1997; Gu et al., 1998; Akhtar and Becker, 2000; Gu et al.,2000; Smith et al., 2000) |
| MLE | RNA/DNA helicase; prefers dsRNA or RNA/DNA hybrid substrates | Helicase activity required for spreading | (Palmer et al., 1994; Lee et al., 1997; Gu et al., 2000; Izzo et al., 2008;Morra et al., 2008) | |
| roX1 | Non-coding RNAs; encoded on the X chromosome | Male lethality in double mutant; MSL complex relocalized to chromocenter | Internal redundancy within each RNA contributes to X chromosome specificity and spreading | (Meller and Rattner, 2002; Stuckenholz et al., 2003; Park et al., 2007; Kelley et al., 2008; Park et al., 2008) |
| roX2 |
| Component . | Protein/RNA type . | Mutant phenotype . | Proposed basis for chromosomal phenotype . | References . |
|---|---|---|---|---|
| MSL1 | Scaffold for MSL complex assembly | Male lethality; no X chromosome recognition | MSL1 and MSL2 are required together for CES recognition; MSL1 is unstable in the absence of MSL2 | (Palmer et al., 1994; Lyman et al., 1997; Chang and Kuroda, 1998; Scott et al., 2000; Morales et al., 2004) |
| MSL2 | RING finger protein | MSL2 is the limiting MSL component in males | (Palmer et al., 1994; Kelley et al., 1995; Lyman et al., 1997) | |
| MSL3 | Chromodomain protein | Chromodomain required for spreading | (Palmer et al., 1994; Buscaino et al., 2006; Sural et al., 2008) | |
| MOF | H4K16 acetyltransferase | Male lethality; partial MSL complex at CES | Histone acetyltransferase activity required for spreading | (Hilfiker et al., 1997; Gu et al., 1998; Akhtar and Becker, 2000; Gu et al.,2000; Smith et al., 2000) |
| MLE | RNA/DNA helicase; prefers dsRNA or RNA/DNA hybrid substrates | Helicase activity required for spreading | (Palmer et al., 1994; Lee et al., 1997; Gu et al., 2000; Izzo et al., 2008;Morra et al., 2008) | |
| roX1 | Non-coding RNAs; encoded on the X chromosome | Male lethality in double mutant; MSL complex relocalized to chromocenter | Internal redundancy within each RNA contributes to X chromosome specificity and spreading | (Meller and Rattner, 2002; Stuckenholz et al., 2003; Park et al., 2007; Kelley et al., 2008; Park et al., 2008) |
| roX2 |
CES, chromatin entry site; dsRNA, double-stranded RNA.