Table 1.

Blastomere injections

Blastomeres
Er'El'Ep'Mv'mr'ml'en'g'Total
Single injections
Biotin-dextran* 56
TRITC or FITC n.a. n.a. n.a. 13
DsRed.T1 mRNA 19
Double injections
TRITC+FITC dextran§ 22
Total 14 12 17 17 15 13 13 110
Blastomeres
Er'El'Ep'Mv'mr'ml'en'g'Total
Single injections
Biotin-dextran* 56
TRITC or FITC n.a. n.a. n.a. 13
DsRed.T1 mRNA 19
Double injections
TRITC+FITC dextran§ 22
Total 14 12 17 17 15 13 13 110

Numbers indicate how many clones were injected as a blastomere with either biotin-dextran, fluorescent-dextran or DsRed.T1 mRNA and analyzed at later stages.

*

Cells were injected with biotin-dextran and embryos were fixed and analyzed at the germband stage or later

Endoderm and visceral mesoderm progenitors were injected with either TRITC-or FITC-labeled dextran and pictures were taken throughout embryogenesis. (No numbers are given for the experiments for TRITC and FITC dextran-injected ectodermal blastomeres, as the resolution with these labels after gastrulation is relatively poor, although what was seen agreed perfectly with the the data obtained using other tracers.)

Cells were injected with mRNA for Discosoma red fluorescent protein and pictures were taken throughout embryogenesis

§

One cell was injected with TRITC-labeled dextran, another one with FITC-labeled dextran. Pictures were taken throughout embryogenesis for endoderm and visceral mesoderm, and up through gastrulation for germline,ectoderm and somatic mesoderm.

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