. | Number of O4+ cells per culture* . | . | . | . | |||
---|---|---|---|---|---|---|---|
. | Treatment . | . | Co-cultures with Q2bn cells . | . | |||
Developmental stage . | No BMP4 . | With BMP4 . | Control cells . | BMP2-producing cells . | |||
E1.5 MNP plus 3 days in vitro† | 120±40 (n=3) | 6±4 (n=3) | 777±313 (n=5) | 7±5 (n=5) | |||
E4.5 VNT§ | 656±128 (n=4) | 199±66 (n=4) | 910±319 (n=4) | 181±87 (n=4) |
. | Number of O4+ cells per culture* . | . | . | . | |||
---|---|---|---|---|---|---|---|
. | Treatment . | . | Co-cultures with Q2bn cells . | . | |||
Developmental stage . | No BMP4 . | With BMP4 . | Control cells . | BMP2-producing cells . | |||
E1.5 MNP plus 3 days in vitro† | 120±40 (n=3) | 6±4 (n=3) | 777±313 (n=5) | 7±5 (n=5) | |||
E4.5 VNT§ | 656±128 (n=4) | 199±66 (n=4) | 910±319 (n=4) | 181±87 (n=4) |
n, number of independent culture experiments.
E1.5 medial neural plate explants aged for 3 days in vitro or E4.5 ventral neural tube explants were cultivated in the absence or presence of human recombinant BMP4 protein (0.6 nM), or co-cultured with Q2bn control cells or Q2bn-BMP2-producing cells. Medial neural plate explants were grown for 4 days and ventral neural tube explants were grown for 7 days. Fixed cultures were then stained with O4 antibody and developing oligodendrocytes were counted.
Mean±s.e.m.
MNP, medial neural plate cultures (four to eight explants per culture).
VNT: ventral neural tube cultures (40 explants per culture).