The Company of Biologists

Table 2.

The function of tissue-specific sma-3 expression constructs in body length regulation

Genotype	Transgene	GFP expression^{^*}	Length(mm)^{^†}	n (worms)
Wild type N2	qcEx50[rol-6]	NA	1.15±0.11	44
sma-3(wk30)	qcEx50[rol-6]	NA	0.74±0.05	40
sma-3(wk30)	qcEx24[sma-3::gfp(N)+rol-6]	H+P+I	1.05±0.08	38
sma-3(wk30)	qcEx42[sma-3::gfp(C)+rol-6]	H+P+I	0.83±0.07	34
Wild type N2	qcEx42[sma-3::gfp(C)+rol-6]	H+P+I	1.03±0.10	51
sma-3(wk30)	qcEx57[sma-3::gfp(C)+sma-3::gfp(N)+rol-6]	H+P+I	1.00±0.11	40
sma-3(wk30)	qcEx44[Pelt-3::sma-3+rol-6]	hyp7	1.04±0.07	40
sma-3(wk30)	qcEx55[Pvha-7::sma-3+rol-6]	hyp7	1.02±0.06	42
sma-3(wk30)	qcEx51[Pdpy-7::sma-3+rol-6]	hyp7	0.94±0.10	32
sma-3(wk30)	qcEx45[Pelt-2::sma-3+rol-6]	Intestine	0.66±0.05	30
sma-3(wk30)	qcEx53[Pvha-6::sma-3+rol-6]	Intestine	0.67±0.04	32
sma-3(wk30)	qcEx52[Pmyo-2::sma-3+rol-6]	Pharynx	0.80±0.06	24
sma-3(wk30)	qcEx54[Ptmy-1(III)::sma-3+rol-6]	Pharynx+Intestine	0.83±0.06	38
sma-3(wk30)	qcEx56[Pmyo-2::sma-3+Pvha-6::sma-3+rol-6]	Pharynx+Intestine	0.80±0.05	27

Genotype	Transgene	GFP expression^{^*}	Length(mm)^{^†}	n (worms)
Wild type N2	qcEx50[rol-6]	NA	1.15±0.11	44
sma-3(wk30)	qcEx50[rol-6]	NA	0.74±0.05	40
sma-3(wk30)	qcEx24[sma-3::gfp(N)+rol-6]	H+P+I	1.05±0.08	38
sma-3(wk30)	qcEx42[sma-3::gfp(C)+rol-6]	H+P+I	0.83±0.07	34
Wild type N2	qcEx42[sma-3::gfp(C)+rol-6]	H+P+I	1.03±0.10	51
sma-3(wk30)	qcEx57[sma-3::gfp(C)+sma-3::gfp(N)+rol-6]	H+P+I	1.00±0.11	40
sma-3(wk30)	qcEx44[Pelt-3::sma-3+rol-6]	hyp7	1.04±0.07	40
sma-3(wk30)	qcEx55[Pvha-7::sma-3+rol-6]	hyp7	1.02±0.06	42
sma-3(wk30)	qcEx51[Pdpy-7::sma-3+rol-6]	hyp7	0.94±0.10	32
sma-3(wk30)	qcEx45[Pelt-2::sma-3+rol-6]	Intestine	0.66±0.05	30
sma-3(wk30)	qcEx53[Pvha-6::sma-3+rol-6]	Intestine	0.67±0.04	32
sma-3(wk30)	qcEx52[Pmyo-2::sma-3+rol-6]	Pharynx	0.80±0.06	24
sma-3(wk30)	qcEx54[Ptmy-1(III)::sma-3+rol-6]	Pharynx+Intestine	0.83±0.06	38
sma-3(wk30)	qcEx56[Pmyo-2::sma-3+Pvha-6::sma-3+rol-6]	Pharynx+Intestine	0.80±0.05	27

NA, not applicable, H+P+I means hypodermis, pharynx and intestine.

The worms are measured 96 hours after synchronization by bleaching. The data shows the average length±standard deviation.