Distribution of HSC/RUs within day 12 AGM
| . | Number of HSC/RUs per tissue . | Number of recipient mice (RM):number of dilutions (D) . |
|---|---|---|
| Aorta (after flushing) | 1.6 (1.2, 2.4) | 10:2 |
| Flushed out of the aorta | 0.3 (0, 1.7) | 11:2 |
| . | Number of HSC/RUs per tissue . | Number of recipient mice (RM):number of dilutions (D) . |
|---|---|---|
| Aorta (after flushing) | 1.6 (1.2, 2.4) | 10:2 |
| Flushed out of the aorta | 0.3 (0, 1.7) | 11:2 |
The dorsal aorta was dissected and contents flushed out using a mouth micropipette. The dorsal aorta was dissociated and transplanted into irradiated recipients in 2 dilutions. Flushed out samples of circulating blood were also transplanted (as two different dilutions) into a separate group of recipients. Numbers of HSC/RUs in tissues were estimated using a limiting dilution method and presented as described in Materials and Methods. These results support the idea that the majority of HSC/RUs in the AGM do not belong to the pool of circulatory HSC/RUs as shown inTable 1.