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Table 2.

Distribution of HSC/RUs within day 12 AGM

Number of HSC/RUs per tissueNumber of recipient mice (RM):number of dilutions (D)
Aorta (after flushing) 1.6 (1.2, 2.4) 10:2 
Flushed out of the aorta 0.3 (0, 1.7) 11:2 
Number of HSC/RUs per tissueNumber of recipient mice (RM):number of dilutions (D)
Aorta (after flushing) 1.6 (1.2, 2.4) 10:2 
Flushed out of the aorta 0.3 (0, 1.7) 11:2 

The dorsal aorta was dissected and contents flushed out using a mouth micropipette. The dorsal aorta was dissociated and transplanted into irradiated recipients in 2 dilutions. Flushed out samples of circulating blood were also transplanted (as two different dilutions) into a separate group of recipients. Numbers of HSC/RUs in tissues were estimated using a limiting dilution method and presented as described in Materials and Methods. These results support the idea that the majority of HSC/RUs in the AGM do not belong to the pool of circulatory HSC/RUs as shown inTable 1.

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