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Table 1.

The comparison of numbers of HSC/RUs found in transplantation experiments by limiting dilution analysis with probable numbers of HSC/RUs in tissues contributed by embryonic circulation*

Embryo ageTissueNumber of benzidine-positive cells (mean±s.d.)Estimated circulating number of HSC/RUs/tissueActual number**of HSC/RUs/tissueNumber of recipient mice (RM): number of dilutions (D)
Day 12 Bled circulation (92±11)×104 3.2 (2.6, 4.2) 43:9 
 AGM (0.8±0.0)×104 0.02 2.7 (1.9, 3.7) 25:7 
 YS (21±4)×104 0.7 1.8 (1.4, 2.4) 56:13 
 Liver (131±12)×104 4.4 53 (43, 69) 37:6 
 Body (w/o liver) nd nd 12.1 (9.1, 19.3) 15:2 
 Body (w/o liver, AGM, PB) (48±6)× 104 1.6 5.8 (4.4, 8.2) 17:3 
 Cord nd nd 0.8 (0.6, 1.8) 7:2 
 Spleen nd nd 7:1 
 Thymus nd nd 2:1 
 Lung (1.9±0.1)× 104 0.1 0.4 (0.2, 0.6) 17:6 
 Limb nd nd 0.5 (0.3, 0.9) 7:2 
 Heart (4.6±3.0)× 104 0.2 2:1 
 Head (18±3)×104 0.6 0.5 (0.4, 0.8) 11:3 
Day 13 Bled circulation (139±22)×104 5.9 (4.7, 7.7) 49:12 
 AGM (1.5±0.3)×104 0.05 0.8 (0.6, 1.2) 17:7 
 YS (24±2)×104 0.8 0.8 (0.6, 1.2) 21:5 
 Liver (205±20)×104 NA 260 (212, 320) 55:12 
 Body (w/o liver) (51±11)×104 1.8 5.6 (4.0, 7.8) 20:3 
 Cord nd nd nd nd 
 Spleen nd nd 0.2 (0.1, 0.4) 12:4 
 Thymus nd nd 12:3 
 Lung (2.0±1.0)×104 0.1 3:1 
 Limb 0.1×104 1.0 (0.8, 1.6) 11:2 
 Heart (6.8±0.4)×104 0.2 3:1 
 Head (11±3)×104 0.4 (0.2)*** 5:1 
Embryo ageTissueNumber of benzidine-positive cells (mean±s.d.)Estimated circulating number of HSC/RUs/tissueActual number**of HSC/RUs/tissueNumber of recipient mice (RM): number of dilutions (D)
Day 12 Bled circulation (92±11)×104 3.2 (2.6, 4.2) 43:9 
 AGM (0.8±0.0)×104 0.02 2.7 (1.9, 3.7) 25:7 
 YS (21±4)×104 0.7 1.8 (1.4, 2.4) 56:13 
 Liver (131±12)×104 4.4 53 (43, 69) 37:6 
 Body (w/o liver) nd nd 12.1 (9.1, 19.3) 15:2 
 Body (w/o liver, AGM, PB) (48±6)× 104 1.6 5.8 (4.4, 8.2) 17:3 
 Cord nd nd 0.8 (0.6, 1.8) 7:2 
 Spleen nd nd 7:1 
 Thymus nd nd 2:1 
 Lung (1.9±0.1)× 104 0.1 0.4 (0.2, 0.6) 17:6 
 Limb nd nd 0.5 (0.3, 0.9) 7:2 
 Heart (4.6±3.0)× 104 0.2 2:1 
 Head (18±3)×104 0.6 0.5 (0.4, 0.8) 11:3 
Day 13 Bled circulation (139±22)×104 5.9 (4.7, 7.7) 49:12 
 AGM (1.5±0.3)×104 0.05 0.8 (0.6, 1.2) 17:7 
 YS (24±2)×104 0.8 0.8 (0.6, 1.2) 21:5 
 Liver (205±20)×104 NA 260 (212, 320) 55:12 
 Body (w/o liver) (51±11)×104 1.8 5.6 (4.0, 7.8) 20:3 
 Cord nd nd nd nd 
 Spleen nd nd 0.2 (0.1, 0.4) 12:4 
 Thymus nd nd 12:3 
 Lung (2.0±1.0)×104 0.1 3:1 
 Limb 0.1×104 1.0 (0.8, 1.6) 11:2 
 Heart (6.8±0.4)×104 0.2 3:1 
 Head (11±3)×104 0.4 (0.2)*** 5:1 
*

Circulatiing and actual numbers of HSCs/RUs per tissue were assessed and presented as described in the Materials and Methods.

**

More than 1e.e. was transplanted per recipient in experiments with only one dilution. Tissues in which no HSC/RUs were detected at least one dilution was more than 1e.e.

***

One recipient was found reconstituted. Numbers of HSC/RUs in tissues are estimated using a limiting dilution method and presented as described in Materials and Methods.

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