Sequences of primers and probes
| Primer name | Primer sequence |
| RT-PCR-Phox2b-F | 5′-TTCCTCTGCCTACGAGTCCTG-3′ |
| RT-PCR-Phox2b-R | 5′-CTCAGCGAAGACCCTCTCCA-3′ |
| RT-PCR-Sox10-F | 5′-GCTGCTGCTATTCAGGCTCAC-3′ |
| RT-PCR-Sox10-R | 5′-CGTAGCCAGCTGCCGAGTAG-3′ |
| RT-PCR-βactin-F | 5′-TGAGAGGGAAATCGTGCGTG-3′ |
| RT-PCR-βactin-R | 5′-TGCTTGCTGATCCACATCTGC-3′ |
| TaqMan-Nkx2.2-F | 5′-GAACAGCAAGCTAGCCGAGG-3′ |
| TaqMan-Nkx2.2-R | 5′-CGATCAGTCCATATAAGGCTGG-3′ |
| TaqMan-Ngn3-F | 5′-ATCCATCACTTTTTCCAGGGTG-3′ |
| TaqMan-Ngn3-R | 5′-TCATCTATGGGCCAAGAGCTG-3′ |
| TaqMan-Phox2b-F | 5′-CTGCAGCCAGGCCAGTG-3′ |
| TaqMan-Phox2b-R | 5′-GGACGGGCAACCGGAC-3′ |
| TaqMan-Insulin(I+II)-F | 5′-AGCGTGGCTTCTTCTACACACC-3′ |
| TaqMan-Insulin(I+II)-R | 5′-CCAGCTCCAGTTGTGCCACT-3′ |
| TaqMan-Glucagon-F | 5′-AGGAATTCATTGCGTGGCTG-3′ |
| TaqMan-Glucagon-R | 5′-CAATGGCGACTTCTTCTGGG-3′ |
| Probe name | Probe sequence |
| TaqMan-Nkx2.2 | 5′-CGCCGCACTCCAGGTTCGTGA-3′ |
| TaqMan-Ngn3 | 5′-AATCCAGTGTTGCGTCTTACCTCACTGGC-3′ |
| TaqMan-Phox2b | 5′-TCCAGTATAACCCGATAAGGACCACTTTTGGG-3′ |
| TaqMan-Insulin(I+II) | 5′-TGGGTCCTCCACTTCACGGCG-3′ |
| TaqMan-Glucagon | 5′-AGTCTCGCCTTCCTCGGCCTTTCA-3′ |
| Primer name | Primer sequence |
| RT-PCR-Phox2b-F | 5′-TTCCTCTGCCTACGAGTCCTG-3′ |
| RT-PCR-Phox2b-R | 5′-CTCAGCGAAGACCCTCTCCA-3′ |
| RT-PCR-Sox10-F | 5′-GCTGCTGCTATTCAGGCTCAC-3′ |
| RT-PCR-Sox10-R | 5′-CGTAGCCAGCTGCCGAGTAG-3′ |
| RT-PCR-βactin-F | 5′-TGAGAGGGAAATCGTGCGTG-3′ |
| RT-PCR-βactin-R | 5′-TGCTTGCTGATCCACATCTGC-3′ |
| TaqMan-Nkx2.2-F | 5′-GAACAGCAAGCTAGCCGAGG-3′ |
| TaqMan-Nkx2.2-R | 5′-CGATCAGTCCATATAAGGCTGG-3′ |
| TaqMan-Ngn3-F | 5′-ATCCATCACTTTTTCCAGGGTG-3′ |
| TaqMan-Ngn3-R | 5′-TCATCTATGGGCCAAGAGCTG-3′ |
| TaqMan-Phox2b-F | 5′-CTGCAGCCAGGCCAGTG-3′ |
| TaqMan-Phox2b-R | 5′-GGACGGGCAACCGGAC-3′ |
| TaqMan-Insulin(I+II)-F | 5′-AGCGTGGCTTCTTCTACACACC-3′ |
| TaqMan-Insulin(I+II)-R | 5′-CCAGCTCCAGTTGTGCCACT-3′ |
| TaqMan-Glucagon-F | 5′-AGGAATTCATTGCGTGGCTG-3′ |
| TaqMan-Glucagon-R | 5′-CAATGGCGACTTCTTCTGGG-3′ |
| Probe name | Probe sequence |
| TaqMan-Nkx2.2 | 5′-CGCCGCACTCCAGGTTCGTGA-3′ |
| TaqMan-Ngn3 | 5′-AATCCAGTGTTGCGTCTTACCTCACTGGC-3′ |
| TaqMan-Phox2b | 5′-TCCAGTATAACCCGATAAGGACCACTTTTGGG-3′ |
| TaqMan-Insulin(I+II) | 5′-TGGGTCCTCCACTTCACGGCG-3′ |
| TaqMan-Glucagon | 5′-AGTCTCGCCTTCCTCGGCCTTTCA-3′ |