Erythroid commitment occurs by day 6 of EB development
| . | % positive cells . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cell surface marker . | EB day 4 . | EB day 5 . | EB day 6 . | EB day 7 . | EB day 8 . | ||||
| Flk1 | 46.7±3.3 | 36.1±0.2 | ND | ND | ND | ||||
| Ter119 | ND | 0.1±0.1 | 7.1±0.5 | 9.3±1.1 | 12.1±0.7 | ||||
| . | % positive cells . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cell surface marker . | EB day 4 . | EB day 5 . | EB day 6 . | EB day 7 . | EB day 8 . | ||||
| Flk1 | 46.7±3.3 | 36.1±0.2 | ND | ND | ND | ||||
| Ter119 | ND | 0.1±0.1 | 7.1±0.5 | 9.3±1.1 | 12.1±0.7 | ||||
Flow cytometric analysis of Flk1 and Ter119 cell-surface marker expression in EBs between days 4 and 8 of differentiation (arithmetic mean of three independent experiments ±s.d.).
ND, not determined.