Transmission analysis of the msi1 mutant allele through the female gametophyte in different transgenic backgrounds
| Line . | Resistant* . | Sensitive . | Expected(%)† . |
|---|---|---|---|
| msi1×wt | 0 | 465 (100%) | 100 |
| msi1/MSI1×PHE1::MSI1 #1 | 0 | 138 (100%) | 0 |
| msi1/MSI1×PHE1::MSI1 #2 | 1 | 221 (99.5%) | 0 |
| msi1/MSI1×PHE1::MSI1 #3 | 1 | 104 (99.0%) | 0 |
| msi1/MSI1; DD46::MSI1/+ #1×wt | 54 | 133 (71.1%) | 66.7‡ |
| msi1/MSI1; DD46::MSI1/+ #2×wt | 34 | 123 (78.3%) | 66.7‡ |
| Line . | Resistant* . | Sensitive . | Expected(%)† . |
|---|---|---|---|
| msi1×wt | 0 | 465 (100%) | 100 |
| msi1/MSI1×PHE1::MSI1 #1 | 0 | 138 (100%) | 0 |
| msi1/MSI1×PHE1::MSI1 #2 | 1 | 221 (99.5%) | 0 |
| msi1/MSI1×PHE1::MSI1 #3 | 1 | 104 (99.0%) | 0 |
| msi1/MSI1; DD46::MSI1/+ #1×wt | 54 | 133 (71.1%) | 66.7‡ |
| msi1/MSI1; DD46::MSI1/+ #2×wt | 34 | 123 (78.3%) | 66.7‡ |
The msi1-1 allele is tagged with a phosphinothricin resistance marker and msi1 transmission was scored by testing resistance to phosphinothricin.
Expected percentages of sensitive seedlings.
In the cross msi1/MSI1; DD46::MSI1/+ × wild type, 25% of the seeds will inherit a msi1 mutant allele from the female, but not a DD46::MSI1 transgene. Those seeds are expected to abort. Among the surviving seeds, 33.3% will inherit the msi1 mutant allele together with the DD46::MSI1 transgene and are expected to transmit the msi1 mutation. Therefore, 66.7% of the seedlings are expected to be phosphinothricin sensitive.