Cumulative data for MO-injection experiments
| . | Nrp1A MO . | Sema3Aa MO . | Sema3Ab MO . | Sema3D MO . | Sema3Fa MO . | Sema3Ga MO . | Sema3Gb MO . | Nrp1A Sema3D MO . | Nrp1A MO + mRNA . | Sema3D MO + mRNA . | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| High conc. | |||||||||||
| ng/nl | 1.0-1.5 | 6-7 | 9-10 | 1.0-1.6 | 7-8 | 7-8 | 7-8 | NA | NA | NA | |
| # of larvae | 25 | 41 | 50 | 34 | 40 | 55 | 37 | NA | NA | NA | |
| Abnormal IPN (%) | 85±4 | 5±1 | 10±5 | 79±8 | 8±6 | 6±1 | 5±1 | NA | NA | NA | |
| Optimal conc. | |||||||||||
| ng/nl | 0.6-0.8 | 4-5 | 7-8 | 0.6-0.8 | 5-6 | 5-6 | 5-6 | 0.3+0.3 | 0.8+0.5 | 0.8+0.5 | |
| # of larvae | 150 | 140 | 121 | 117 | 133 | 103 | 128 | 71 | 42 | 41 | |
| Abnormal IPN (%) | 75±5 | 3±2 | 8±3 | 66±5 | 6±2 | 7±2 | 6±1 | 68±6 | 41±9 | 33±9 | |
| Low conc. | |||||||||||
| ng/nl | 0.3 | 2 | 5 | 0.3 | 3 | 3 | 3 | NA | NA | NA | |
| # of larvae | 114 | 59 | 25 | 63 | 31 | 37 | 36 | NA | NA | NA | |
| Abnormal IPN (%) | 25±6 | 3±0.6 | 8±0.5 | 27±3 | 7±0.5 | 5±0.5 | 6±0.5 | NA | NA | NA | |
| Mock injection | |||||||||||
| # of larvae | 202 | ||||||||||
| Abnormal IPN (%) | 4±0.4 | ||||||||||
| No injection | |||||||||||
| # of larvae | 257 | ||||||||||
| Abnormal IPN (%) | 2±1.5 | ||||||||||
| . | Nrp1A MO . | Sema3Aa MO . | Sema3Ab MO . | Sema3D MO . | Sema3Fa MO . | Sema3Ga MO . | Sema3Gb MO . | Nrp1A Sema3D MO . | Nrp1A MO + mRNA . | Sema3D MO + mRNA . | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| High conc. | |||||||||||
| ng/nl | 1.0-1.5 | 6-7 | 9-10 | 1.0-1.6 | 7-8 | 7-8 | 7-8 | NA | NA | NA | |
| # of larvae | 25 | 41 | 50 | 34 | 40 | 55 | 37 | NA | NA | NA | |
| Abnormal IPN (%) | 85±4 | 5±1 | 10±5 | 79±8 | 8±6 | 6±1 | 5±1 | NA | NA | NA | |
| Optimal conc. | |||||||||||
| ng/nl | 0.6-0.8 | 4-5 | 7-8 | 0.6-0.8 | 5-6 | 5-6 | 5-6 | 0.3+0.3 | 0.8+0.5 | 0.8+0.5 | |
| # of larvae | 150 | 140 | 121 | 117 | 133 | 103 | 128 | 71 | 42 | 41 | |
| Abnormal IPN (%) | 75±5 | 3±2 | 8±3 | 66±5 | 6±2 | 7±2 | 6±1 | 68±6 | 41±9 | 33±9 | |
| Low conc. | |||||||||||
| ng/nl | 0.3 | 2 | 5 | 0.3 | 3 | 3 | 3 | NA | NA | NA | |
| # of larvae | 114 | 59 | 25 | 63 | 31 | 37 | 36 | NA | NA | NA | |
| Abnormal IPN (%) | 25±6 | 3±0.6 | 8±0.5 | 27±3 | 7±0.5 | 5±0.5 | 6±0.5 | NA | NA | NA | |
| Mock injection | |||||||||||
| # of larvae | 202 | ||||||||||
| Abnormal IPN (%) | 4±0.4 | ||||||||||
| No injection | |||||||||||
| # of larvae | 257 | ||||||||||
| Abnormal IPN (%) | 2±1.5 | ||||||||||
The total number (#) of larvae examined for each MO condition and the concentrations (conc.) injected in ng per nl are indicated. The average percentage±s.d. of larvae lacking dorsal IPN innervation (Abnormal IPN%) was calculated from at least three repeated trials.
NA, not applicable.