PCR primer sequences
| Gene . | Forward (5′ to 3′) . | Reverse (5′ to 3′) . |
|---|---|---|
| RT-PCR primer sequences | ||
| Actb | GGCATTGTTACCAACTGGGACGAC | CCAGAGGCATACAGGGACAGCACAG |
| Plcz1 | TGACGACCATCCAGTTACCCTCAC | ACAACACGAGTCTGCTGCTTCACG |
| Venus | ACGTAAACGGCCACAAGTTC | GAACTCCAGCAGGACCATGT |
| Plac1 | TCCACACGGAGAGAACAGAACTAAC | AGAAGGCGTCCAGGAAGGATTC |
| Gcm1 | AAAGCCAGACAGAAGCAGCAGAGG | AAAAGATGAAGCGTCCGTCGTGCC |
| Zfp36l3 | CAGTGATGACGAAGATGAAGACGAC | TGAGAAGCGGCTGAAGATGG |
| Plib (Prl3d2) | TTGGATAACAGACAGAACACTTCCC | CAGTCAGTGCAGACACCAGGTG |
| Tpbpa | CACAGCCAGTTGTTGATGACCC | TTTTTGCTTGCCCTTGCCCCAG |
| Ereg | TGGTCTGCGATGTGAGCACTTC | CCTTGTCCGTAACTTGATGGCAC |
| Ptgs2 | CAACACACTCTATCACTGGCACCC | CATCTCTCTGCTCTGGTCAATGG |
| Mmp1a | CTTCAAAGGCAGGTTCTACATTCG | TCTTCCTCACAAACAGCAGCATC |
| Mmp2 | CCTGGAATGCCATCCCTGATAAC | TAACTACAGAGGAGGACAGAGCCG |
| Eomes | ATCGTGGAAGTGACAGAGGACG | CGGGAAGAAGTTTTGAACGCC |
| Hand1 | CAAGGCTGAACTCAAAAAGACGG | TCTCACTGGTTTAGCTCCAGCG |
| Psx1 (Rhox6) | TGAATAGGCTGGCTCAACTGCG | AAAGGGCTCTCCTCATCCGAAACC |
| Ets2 | CAAGGCAAACCAGTTATTCCTGC | TTTTTCCTCTTCCCCCACCG |
| PLCZ1 | CAAATGAAGCAGCAGACTCGTG | ACGTATCAATGCCAATTCTGGG |
| ACTB | TGACAGGATGCAGAAGGAGA | GCTGGAAGGTGGACAGTGAG |
| Cre | CATTTGGGCCAGCTAAACAT | CCCGGCAAAACAGGTAGTTA |
| qPCR (RT-PCR) primer sequences | ||
| Actb | TGACAGGATGCAGAAGGAGA | GCTGGAAGGTGGACAGTGAG |
| Pou5f1 | CGTGAAGTTGGAGAAGGTGGAACC | GCAGCTTGGCAAACTGTTCTAGCTC |
| Mos | CAGTGGTTGCCTACAATCTGCG | AGCCTTGAGGTCCCTTTGGAG |
| Nanog | GCAAGCGGTGGCAGAAAAAC | GCAATGGATGCTGGGATACTCC |
| Cdx2 | GCAGTCCCTAGGAAGCCAAGTG | CTCTCGGAGAGCCCAAGTGTG |
| Sox2 | GGAAAAAAACCACCAATCCCATCC | TTTGCGAACTCCCTGCGAAG |
| Fbxo43 | AGTGGTGAGCAGGTTCCAACTCTG | TGTTTACTCCGTAGGTGGGTGAGG |
| Plcz1 | TGACGACCATCCAGTTACCCTCAC | ACAACACGAGTCTGCTGCTTCACG |
| Igf2 | CTAAGACTTGGATCCCAGAACC | GTTCTTCTCCTTGGGTTCTTTC |
| H19 | TTGCACTAAGTCGATTGCACT | GGAACTGCTTCCAGACTAGGC |
| Mest | GGCTCCTCTATGATGGCCG | AAGCCTTTCTGAACAGCCAGC |
| Peg3 | CAATCTATGAATGCCAGGACTGTG | CGACTGTCAACCAGAGCCTTTC |
| Nnat | CGAAAGCCCTCCCCAAAATG | TTGACCACAACTGCTGCGTGAGAC |
| Peg10 | TGATGCCTCCAAACAGCCAGAC | CCTCCATTGCCACAGTAGAGACAC |
| Peg12 | TTGCACATTTCCTGTGGGAC | GGGTCAGAAGGAGGAAATCAACTC |
| Peg13 | ACTCAGAAGGGCATCTCACCTCTC | TTTGGCTTGGTTTGTTCACACC |
| Grb10 | AATGGGTCCCCCAAGTTTTCT | CCGCAACCTGCAGAGAGC |
| Air | TGAGCAGGCACATTACCGAAGG | TTTTCCCCCACCCCTAAAGC |
| Cdkn1c | AGGAGCAGGACGAGAATCAAGAGC | GAAGAAGTCGTTCGCATTGGC |
| Impact | ACCGAAGGAGCACTTTTCAGGC | TAGGCATAGATGTTGTGGGTGGCG |
| Plag1 | GGACTTCCTGTGTCAGTTCTGTGC | TGCTCTGGTAATCTCCTGCCTG |
| Ascl2 | TTCCAGTTGGTTAGGGGGCTAC | CTTGGCATTTGGTCAGGCTG |
| Pon2 | AACCATCCTCCTGCATCAGAGGTC | GGACAGACCCGTTGTTGATATACAC |
| Copg2 | GCAGAGACAGAGTATTTCGTTCGC | CACTTCATAGGAATCGGATGGTTC |
| Gtl2 | TTGCACATTTCCTGTGGGAC | AAGCACCATGAGCCACTAGG |
| Zim1 | CCAAAGAGAACGATGTTCCTGG | TGTCTTAGAATTGTCTGGCTTCCG |
| Htr2a | CTGAAAATCATTGCGGTGTGG | AAAGTTGTCATCGGCGAGCAGG |
| Dppa2 | CGATGTCCTCTTCCGTCTAATGTG | AACCCAGGTCTGTCCAGCAAG |
| NM_026894 | ATCTCATCAATCGTCAGACCTTCC | CCACCCTTTCCACATTTTGTTCAG |
| Rbp1 | ATCCGCACGCTGAGCACTTTTC | CACTGGAGTTTGTCACCATCCC |
| Dab2 | CTTCAAAGGCAATGCTCCTCC | TTATGGCTCCTGGGACCACAGTTG |
| Aurka | GCGGGAGAGACAAAGCAAGTTC | ATACAGCCTGAGGATGTTGGGGTG |
| Ptgs1 | CGTTCACCCATTTCCTGCTGAC | GATAAGGTTGGACCGCACTGTG |
| Ptgs2 | TCTCTACAACAACTCCATCCTCCTG | CACATTTCTTCCCCCAGCAAC |
| Elavl1 | TTTCTCGGTTTGGGCGAATC | CCTGGGGGTTTATGACCATTG |
| Plk1 | GTATTCCCAAGCACATCAACCCAG | GCCAGAAGTGAAGAACTCGTCATTG |
| Cd24a | TGCTCCTACCCACGCAGATTTAC | GTTACTTGGATTTGGGGAAGCAG |
| Kras | AACTGGGGAGGGCTTTCTTTG | ACCATAGGCACATCTTCAGAGTCC |
| Myc | TCCTTTGGGCGTTGGAAACC | TCGTCGCAGATGAAATAGGGC |
| Akt1 | TTGTGTCTGCCCTGGACTACTTGC | CCGTTATCTTGATGTGCCCGTC |
| Trp53 | TGGAAGACAGGCAGACTTTTCG | ATGATGGTAAGGATAGGTCGGCGG |
| Pcna | AAGAAGAGGAGGCGGTAACCATAG | GGAGACAGTGGAGTGGCTTTTG |
| Atm | CATAGACCTGGGAGTGGCTTTTG | TCCATCGTTTTTTCACAGCACC |
| Atr | CGAATGGGAAAAAGGTGGTCG | TGGTGCTAACCGATTTGTGTGC |
| Cdkn1a | GCAGACCAGCCTGACAGATTTC | TCCTGACCCACAGCAGAAGAGG |
| Chk1 (Chek1) | GCTTTCCTTGTGGGACACTGGTC | GCCAGGGGTTCTGTGAAGATCC |
| Brca1 | TCAGGCTTGACCCCCAAAGAAG | TCACACACAAACTCCGCATCTG |
| Brca2 | GACCACGAAGAAGACACAACACAG | CGCTGAAACAAGCCTCAAGGTG |
| Id2 | AGCCTGCATCACCAGAGACCTG | TCATTCGACATAAGCTCAGAAGGG |
| Gcm1 | AAAGCCAGACAGAAGCAGCAGAGG | AAAAGATGAAGCGTCCGTCGTGCC |
| Primer sequences for estimation of tg dosage | ||
| Actb | TGACAGGATGCAGAAGGAGA | GCTGGAAGGTGGACAGTGAG |
| H2afz | GCGTATCACCCCTCGTCACTTG | TCTTCTGTTGTCCTTTCTTCCCG |
| Plcz1 | TGACGACCATCCAGTTACCCTCAC | ACAACACGAGTCTGCTGCTTCACG |
| Venus | GCAGAAGAACGGCATCAAGGCC | TGCTCAGGTAGTGGTTGTCGGG |
| Primer sequences for promoter sequencing | ||
| Plcz1 | TCAGAGGTCACCCAACACGG | |
| TTTTCCCTTGGTTCACGGC | ||
| TGGTAGCGGGGAACTTTAC | ||
| CCAAGACACCTCTCTAACATCC | ||
| TTACCCTTTTTGCTGTGTTGGTG | ||
| CCGTTCCAACACTCTCCATTGTC | ||
| GTATGCTGGTCTATGAAGAAGG | ||
| CCTGGGGGTAAACATTGTGATGAA | ||
| GATTTCATGATCTGGGCCGCT | ||
| CAGACACAGAGAAAACATACCAC | ||
| GCCATCAGCACTGCCAAAAATACTG | ||
| GCAATGTGCTGAGAAAAACAG | ||
| GGGGAAGAATAAGAAATCGTG | ||
| TCCTTGGGCTACATAGTGC | ||
| AGCACTCACCAGACCGAAG | ||
| AGATGACAGTCCCTCTGCCG |
| Gene . | Forward (5′ to 3′) . | Reverse (5′ to 3′) . |
|---|---|---|
| RT-PCR primer sequences | ||
| Actb | GGCATTGTTACCAACTGGGACGAC | CCAGAGGCATACAGGGACAGCACAG |
| Plcz1 | TGACGACCATCCAGTTACCCTCAC | ACAACACGAGTCTGCTGCTTCACG |
| Venus | ACGTAAACGGCCACAAGTTC | GAACTCCAGCAGGACCATGT |
| Plac1 | TCCACACGGAGAGAACAGAACTAAC | AGAAGGCGTCCAGGAAGGATTC |
| Gcm1 | AAAGCCAGACAGAAGCAGCAGAGG | AAAAGATGAAGCGTCCGTCGTGCC |
| Zfp36l3 | CAGTGATGACGAAGATGAAGACGAC | TGAGAAGCGGCTGAAGATGG |
| Plib (Prl3d2) | TTGGATAACAGACAGAACACTTCCC | CAGTCAGTGCAGACACCAGGTG |
| Tpbpa | CACAGCCAGTTGTTGATGACCC | TTTTTGCTTGCCCTTGCCCCAG |
| Ereg | TGGTCTGCGATGTGAGCACTTC | CCTTGTCCGTAACTTGATGGCAC |
| Ptgs2 | CAACACACTCTATCACTGGCACCC | CATCTCTCTGCTCTGGTCAATGG |
| Mmp1a | CTTCAAAGGCAGGTTCTACATTCG | TCTTCCTCACAAACAGCAGCATC |
| Mmp2 | CCTGGAATGCCATCCCTGATAAC | TAACTACAGAGGAGGACAGAGCCG |
| Eomes | ATCGTGGAAGTGACAGAGGACG | CGGGAAGAAGTTTTGAACGCC |
| Hand1 | CAAGGCTGAACTCAAAAAGACGG | TCTCACTGGTTTAGCTCCAGCG |
| Psx1 (Rhox6) | TGAATAGGCTGGCTCAACTGCG | AAAGGGCTCTCCTCATCCGAAACC |
| Ets2 | CAAGGCAAACCAGTTATTCCTGC | TTTTTCCTCTTCCCCCACCG |
| PLCZ1 | CAAATGAAGCAGCAGACTCGTG | ACGTATCAATGCCAATTCTGGG |
| ACTB | TGACAGGATGCAGAAGGAGA | GCTGGAAGGTGGACAGTGAG |
| Cre | CATTTGGGCCAGCTAAACAT | CCCGGCAAAACAGGTAGTTA |
| qPCR (RT-PCR) primer sequences | ||
| Actb | TGACAGGATGCAGAAGGAGA | GCTGGAAGGTGGACAGTGAG |
| Pou5f1 | CGTGAAGTTGGAGAAGGTGGAACC | GCAGCTTGGCAAACTGTTCTAGCTC |
| Mos | CAGTGGTTGCCTACAATCTGCG | AGCCTTGAGGTCCCTTTGGAG |
| Nanog | GCAAGCGGTGGCAGAAAAAC | GCAATGGATGCTGGGATACTCC |
| Cdx2 | GCAGTCCCTAGGAAGCCAAGTG | CTCTCGGAGAGCCCAAGTGTG |
| Sox2 | GGAAAAAAACCACCAATCCCATCC | TTTGCGAACTCCCTGCGAAG |
| Fbxo43 | AGTGGTGAGCAGGTTCCAACTCTG | TGTTTACTCCGTAGGTGGGTGAGG |
| Plcz1 | TGACGACCATCCAGTTACCCTCAC | ACAACACGAGTCTGCTGCTTCACG |
| Igf2 | CTAAGACTTGGATCCCAGAACC | GTTCTTCTCCTTGGGTTCTTTC |
| H19 | TTGCACTAAGTCGATTGCACT | GGAACTGCTTCCAGACTAGGC |
| Mest | GGCTCCTCTATGATGGCCG | AAGCCTTTCTGAACAGCCAGC |
| Peg3 | CAATCTATGAATGCCAGGACTGTG | CGACTGTCAACCAGAGCCTTTC |
| Nnat | CGAAAGCCCTCCCCAAAATG | TTGACCACAACTGCTGCGTGAGAC |
| Peg10 | TGATGCCTCCAAACAGCCAGAC | CCTCCATTGCCACAGTAGAGACAC |
| Peg12 | TTGCACATTTCCTGTGGGAC | GGGTCAGAAGGAGGAAATCAACTC |
| Peg13 | ACTCAGAAGGGCATCTCACCTCTC | TTTGGCTTGGTTTGTTCACACC |
| Grb10 | AATGGGTCCCCCAAGTTTTCT | CCGCAACCTGCAGAGAGC |
| Air | TGAGCAGGCACATTACCGAAGG | TTTTCCCCCACCCCTAAAGC |
| Cdkn1c | AGGAGCAGGACGAGAATCAAGAGC | GAAGAAGTCGTTCGCATTGGC |
| Impact | ACCGAAGGAGCACTTTTCAGGC | TAGGCATAGATGTTGTGGGTGGCG |
| Plag1 | GGACTTCCTGTGTCAGTTCTGTGC | TGCTCTGGTAATCTCCTGCCTG |
| Ascl2 | TTCCAGTTGGTTAGGGGGCTAC | CTTGGCATTTGGTCAGGCTG |
| Pon2 | AACCATCCTCCTGCATCAGAGGTC | GGACAGACCCGTTGTTGATATACAC |
| Copg2 | GCAGAGACAGAGTATTTCGTTCGC | CACTTCATAGGAATCGGATGGTTC |
| Gtl2 | TTGCACATTTCCTGTGGGAC | AAGCACCATGAGCCACTAGG |
| Zim1 | CCAAAGAGAACGATGTTCCTGG | TGTCTTAGAATTGTCTGGCTTCCG |
| Htr2a | CTGAAAATCATTGCGGTGTGG | AAAGTTGTCATCGGCGAGCAGG |
| Dppa2 | CGATGTCCTCTTCCGTCTAATGTG | AACCCAGGTCTGTCCAGCAAG |
| NM_026894 | ATCTCATCAATCGTCAGACCTTCC | CCACCCTTTCCACATTTTGTTCAG |
| Rbp1 | ATCCGCACGCTGAGCACTTTTC | CACTGGAGTTTGTCACCATCCC |
| Dab2 | CTTCAAAGGCAATGCTCCTCC | TTATGGCTCCTGGGACCACAGTTG |
| Aurka | GCGGGAGAGACAAAGCAAGTTC | ATACAGCCTGAGGATGTTGGGGTG |
| Ptgs1 | CGTTCACCCATTTCCTGCTGAC | GATAAGGTTGGACCGCACTGTG |
| Ptgs2 | TCTCTACAACAACTCCATCCTCCTG | CACATTTCTTCCCCCAGCAAC |
| Elavl1 | TTTCTCGGTTTGGGCGAATC | CCTGGGGGTTTATGACCATTG |
| Plk1 | GTATTCCCAAGCACATCAACCCAG | GCCAGAAGTGAAGAACTCGTCATTG |
| Cd24a | TGCTCCTACCCACGCAGATTTAC | GTTACTTGGATTTGGGGAAGCAG |
| Kras | AACTGGGGAGGGCTTTCTTTG | ACCATAGGCACATCTTCAGAGTCC |
| Myc | TCCTTTGGGCGTTGGAAACC | TCGTCGCAGATGAAATAGGGC |
| Akt1 | TTGTGTCTGCCCTGGACTACTTGC | CCGTTATCTTGATGTGCCCGTC |
| Trp53 | TGGAAGACAGGCAGACTTTTCG | ATGATGGTAAGGATAGGTCGGCGG |
| Pcna | AAGAAGAGGAGGCGGTAACCATAG | GGAGACAGTGGAGTGGCTTTTG |
| Atm | CATAGACCTGGGAGTGGCTTTTG | TCCATCGTTTTTTCACAGCACC |
| Atr | CGAATGGGAAAAAGGTGGTCG | TGGTGCTAACCGATTTGTGTGC |
| Cdkn1a | GCAGACCAGCCTGACAGATTTC | TCCTGACCCACAGCAGAAGAGG |
| Chk1 (Chek1) | GCTTTCCTTGTGGGACACTGGTC | GCCAGGGGTTCTGTGAAGATCC |
| Brca1 | TCAGGCTTGACCCCCAAAGAAG | TCACACACAAACTCCGCATCTG |
| Brca2 | GACCACGAAGAAGACACAACACAG | CGCTGAAACAAGCCTCAAGGTG |
| Id2 | AGCCTGCATCACCAGAGACCTG | TCATTCGACATAAGCTCAGAAGGG |
| Gcm1 | AAAGCCAGACAGAAGCAGCAGAGG | AAAAGATGAAGCGTCCGTCGTGCC |
| Primer sequences for estimation of tg dosage | ||
| Actb | TGACAGGATGCAGAAGGAGA | GCTGGAAGGTGGACAGTGAG |
| H2afz | GCGTATCACCCCTCGTCACTTG | TCTTCTGTTGTCCTTTCTTCCCG |
| Plcz1 | TGACGACCATCCAGTTACCCTCAC | ACAACACGAGTCTGCTGCTTCACG |
| Venus | GCAGAAGAACGGCATCAAGGCC | TGCTCAGGTAGTGGTTGTCGGG |
| Primer sequences for promoter sequencing | ||
| Plcz1 | TCAGAGGTCACCCAACACGG | |
| TTTTCCCTTGGTTCACGGC | ||
| TGGTAGCGGGGAACTTTAC | ||
| CCAAGACACCTCTCTAACATCC | ||
| TTACCCTTTTTGCTGTGTTGGTG | ||
| CCGTTCCAACACTCTCCATTGTC | ||
| GTATGCTGGTCTATGAAGAAGG | ||
| CCTGGGGGTAAACATTGTGATGAA | ||
| GATTTCATGATCTGGGCCGCT | ||
| CAGACACAGAGAAAACATACCAC | ||
| GCCATCAGCACTGCCAAAAATACTG | ||
| GCAATGTGCTGAGAAAAACAG | ||
| GGGGAAGAATAAGAAATCGTG | ||
| TCCTTGGGCTACATAGTGC | ||
| AGCACTCACCAGACCGAAG | ||
| AGATGACAGTCCCTCTGCCG |