Oligonucleotide primers
| Amplified sequence . | Forward (F) . | Reverse (R) . |
|---|---|---|
| PU.1+ (genomic) | GGCCTACAGGAAGAGCAAGCCTGGCA | ATTCGTTACAAATGCAAGCTAAA |
| PU.1G (genomic) | AGGGGCGGTGAGGGAAAACCCTTCCA | ATTCGTTACAAATGCAAGCTAAA |
| PU.1 (RNA) | TGGGAGGGTTTTCCCTCACC | TGCTGTCCTTCATGTCGCCG |
| huCD4 | CCTCAGTGCAATGTAGGAGTC | CTCATCAGATGCATATTCTGC |
| Stra8 | TCACAGCCTCAAAGTGGCAGG | GCAACAGAGTGGAGGAG |
| Gapdh | CACCACCAACTGCTTAGCC | CGGATACATTGGGGGTAGG |
| Kit | CAACAGCAATGGCCTCACGAGT | GTGGTCACCTTTGCTCTGCTC |
| Sycp1 | ATGGAGAAGCAAAAGCCCTTC | ATGTTTTCCCGACTGGACATTG |
| Prm1 | GGCCAGATACCGATGC | CCTTAGCAGGCTCCTG |
| β-actin | AAGGCCAACCGTGAAAAGAT | GTGGTACGACCAGAGGCATAC |
| Cd81 | GGGCATCTACATTCTCATTGC | GTATGGTGGTAGTCAGTGTGG |
| Cd97 | CTCGGAGAAGGAATTGATCAC | CTTCTGAGTCTCCCATCTGTG |
| Nptn | GAATTGTCACCAGTGAAGAGG | CATCATAGCATCCTGCCCTTC |
| Fos | CAGCCTTTCCTACTACCATTC | CTGACACGGTCTTCACCATTC |
| Cdc42 | GGCAAGAGGATTATGACAGAC | CAGCAGTCTCTGGAGTAATAG |
| Cd14 | GGAAGCCAGAGAACACCATCG | GCAGGGCTCCGAATAGAATCC |
| Lyn | GAGAGAAGGCTTCATCCCCAG | GTCTTCACAGCCACCTTTGTG |
| Nek7 | GAGCATCCTGTCTCTTGGATG | GACTCTTCGAGAATGCATGTG |
| stella | AAAGCTAACCCTAAACCCCGG | TGGCTCACTGTCCCGTTCA |
| vasa | CCAAAAGTGACATATATACCC | TTGGTTGATCAGTTCTCGAG |
| Plzf | CCACCTTCGCTCACATACAG | TGTGGCTCTTGAGTGTGCTC |
| Taf4b | CAGACAGCCAAAGTCAAGCA | TGGAGGTCACATGATTCACAC |
| Oct4 | TGAGGCTACAGGACACCTTTC | GTGCCAAAGTGGGGACCT |
| Dazl | GCTGATATTTTGCCCAATGAA | ATGCTTCGGTCCACAGACTT |
| Amplified sequence . | Forward (F) . | Reverse (R) . |
|---|---|---|
| PU.1+ (genomic) | GGCCTACAGGAAGAGCAAGCCTGGCA | ATTCGTTACAAATGCAAGCTAAA |
| PU.1G (genomic) | AGGGGCGGTGAGGGAAAACCCTTCCA | ATTCGTTACAAATGCAAGCTAAA |
| PU.1 (RNA) | TGGGAGGGTTTTCCCTCACC | TGCTGTCCTTCATGTCGCCG |
| huCD4 | CCTCAGTGCAATGTAGGAGTC | CTCATCAGATGCATATTCTGC |
| Stra8 | TCACAGCCTCAAAGTGGCAGG | GCAACAGAGTGGAGGAG |
| Gapdh | CACCACCAACTGCTTAGCC | CGGATACATTGGGGGTAGG |
| Kit | CAACAGCAATGGCCTCACGAGT | GTGGTCACCTTTGCTCTGCTC |
| Sycp1 | ATGGAGAAGCAAAAGCCCTTC | ATGTTTTCCCGACTGGACATTG |
| Prm1 | GGCCAGATACCGATGC | CCTTAGCAGGCTCCTG |
| β-actin | AAGGCCAACCGTGAAAAGAT | GTGGTACGACCAGAGGCATAC |
| Cd81 | GGGCATCTACATTCTCATTGC | GTATGGTGGTAGTCAGTGTGG |
| Cd97 | CTCGGAGAAGGAATTGATCAC | CTTCTGAGTCTCCCATCTGTG |
| Nptn | GAATTGTCACCAGTGAAGAGG | CATCATAGCATCCTGCCCTTC |
| Fos | CAGCCTTTCCTACTACCATTC | CTGACACGGTCTTCACCATTC |
| Cdc42 | GGCAAGAGGATTATGACAGAC | CAGCAGTCTCTGGAGTAATAG |
| Cd14 | GGAAGCCAGAGAACACCATCG | GCAGGGCTCCGAATAGAATCC |
| Lyn | GAGAGAAGGCTTCATCCCCAG | GTCTTCACAGCCACCTTTGTG |
| Nek7 | GAGCATCCTGTCTCTTGGATG | GACTCTTCGAGAATGCATGTG |
| stella | AAAGCTAACCCTAAACCCCGG | TGGCTCACTGTCCCGTTCA |
| vasa | CCAAAAGTGACATATATACCC | TTGGTTGATCAGTTCTCGAG |
| Plzf | CCACCTTCGCTCACATACAG | TGTGGCTCTTGAGTGTGCTC |
| Taf4b | CAGACAGCCAAAGTCAAGCA | TGGAGGTCACATGATTCACAC |
| Oct4 | TGAGGCTACAGGACACCTTTC | GTGCCAAAGTGGGGACCT |
| Dazl | GCTGATATTTTGCCCAATGAA | ATGCTTCGGTCCACAGACTT |
Sequences are shown 5′ to 3′.