The Company of Biologists

Table 1.

RNAi of OID genes disrupts cortical granule trafficking

RNAi	Class	Function	Oocytes	Embryo +1	Embryo +2
Control	-	-	Normal	Normal	Absent
wee-1.3	Cell cycle	CDK activation	UGTP-1 agg., normal CGs	Abnormal vesicles	Abnormal vesicles
czw-1	Cell cycle	SAC, membrane traffic	UGTP-1 puncta, small CGs	Abnormal vesicles	Abnormal vesicles
syn-4	Membrane traffic	Vesicle fusion	Vesicles at cortex	Abnormal vesicles	Abnormal vesicles
cyk-3	Cell cycle	Deubiquitination enzyme	Normal	CG clusters	CG clusters
apc-2	Cell cycle	Ubiquitin ligase	Normal	CG clusters	CG clusters
sep-1	Cell cycle	Chromosome segregation	Normal	Normal	Retained CGs
ify-1	Cell cycle	Separase regulation	Normal	Normal	Retained CGs
cdk-1	Cell cycle	Entry into M phase	Normal	Normal	Retained CGs
cks-1	Cell cycle	Cell cycle exit/cytokinesis	Normal	Normal	Absent
chs-1	Cargo	Chitin synthesis	Normal	Normal	Absent
sqv-4	Cargo	Glycosylation enzyme	Small CGs	Small CGs	Absent
cpg-1/-2	Cargo	Chitin-binding proteins	Small CGS	Small CGs	Absent

RNAi	Class	Function	Oocytes	Embryo +1	Embryo +2
Control	-	-	Normal	Normal	Absent
wee-1.3	Cell cycle	CDK activation	UGTP-1 agg., normal CGs	Abnormal vesicles	Abnormal vesicles
czw-1	Cell cycle	SAC, membrane traffic	UGTP-1 puncta, small CGs	Abnormal vesicles	Abnormal vesicles
syn-4	Membrane traffic	Vesicle fusion	Vesicles at cortex	Abnormal vesicles	Abnormal vesicles
cyk-3	Cell cycle	Deubiquitination enzyme	Normal	CG clusters	CG clusters
apc-2	Cell cycle	Ubiquitin ligase	Normal	CG clusters	CG clusters
sep-1	Cell cycle	Chromosome segregation	Normal	Normal	Retained CGs
ify-1	Cell cycle	Separase regulation	Normal	Normal	Retained CGs
cdk-1	Cell cycle	Entry into M phase	Normal	Normal	Retained CGs
cks-1	Cell cycle	Cell cycle exit/cytokinesis	Normal	Normal	Absent
chs-1	Cargo	Chitin synthesis	Normal	Normal	Absent
sqv-4	Cargo	Glycosylation enzyme	Small CGs	Small CGs	Absent
cpg-1/-2	Cargo	Chitin-binding proteins	Small CGS	Small CGs	Absent