Forward (F) and reverse (R) primers used to synthesize IP3R1 GST-fusion proteins
| Primer name . | 5′ restriction site . | Sequence 5′-3′ . |
|---|---|---|
| Domain 1F | BamHI | ACTAAAGGATCCATGTCTGACAAAATGTCG |
| Domain 1R | EcoRI | CAAGGAATTCCCTAGTCTCAACCTACTCCGAGA |
| Domain 2F | BamHI | ACTAAAGGATCCAACGCGCAAGAAAAAATG |
| Domain 2R | EcoRI | CAAGGAATTCCCTAGCCTCATCACGTTACTGCC |
| Domain 3F | EcoRI | GACGGAATTCCCTCTATCCATGGCGTTGGG |
| Domain 3R | XhoI | CAAGCTCGAGCGTAGTCGGGCTGATAACCGCCA |
| Domain 4F | BamHI | ACTAAAGGATCCAACGCCGCTCGCAGAGAC |
| Domain 4R | EcoRI | CAAGGAATTCCCTAGCCTCCGGAAGGTGGTAAA |
| Domain 5F | XhoI | GACACTCGAGCGGAGGCCGACCCTGATGAC |
| Domain 5R | NotI | AACGCGGCCGCATAGGTCGATGATGACCCCGAA |
| Domain 6F | BamHI | ACTAAAGGATCCACCTTTGCTGACCTGAGG |
| Domain 6R | EcoRI | CAAGGAATTCCCTAGGGCCCGGCTGCTGTGGGTT |
| Primer name . | 5′ restriction site . | Sequence 5′-3′ . |
|---|---|---|
| Domain 1F | BamHI | ACTAAAGGATCCATGTCTGACAAAATGTCG |
| Domain 1R | EcoRI | CAAGGAATTCCCTAGTCTCAACCTACTCCGAGA |
| Domain 2F | BamHI | ACTAAAGGATCCAACGCGCAAGAAAAAATG |
| Domain 2R | EcoRI | CAAGGAATTCCCTAGCCTCATCACGTTACTGCC |
| Domain 3F | EcoRI | GACGGAATTCCCTCTATCCATGGCGTTGGG |
| Domain 3R | XhoI | CAAGCTCGAGCGTAGTCGGGCTGATAACCGCCA |
| Domain 4F | BamHI | ACTAAAGGATCCAACGCCGCTCGCAGAGAC |
| Domain 4R | EcoRI | CAAGGAATTCCCTAGCCTCCGGAAGGTGGTAAA |
| Domain 5F | XhoI | GACACTCGAGCGGAGGCCGACCCTGATGAC |
| Domain 5R | NotI | AACGCGGCCGCATAGGTCGATGATGACCCCGAA |
| Domain 6F | BamHI | ACTAAAGGATCCACCTTTGCTGACCTGAGG |
| Domain 6R | EcoRI | CAAGGAATTCCCTAGGGCCCGGCTGCTGTGGGTT |
Restriction sites are underlined and the stop codons are indicated in bold.