Phenotypic analysis of CMP-FLEC derived hepatocytes colonies
| . | . | . | Phenotypes of donor cell colonies . | . | . | ||
|---|---|---|---|---|---|---|---|
| Reactivity . | Marker . | Assay . | Three months post-transplant CMP-FLECII,III,IV . | Six months post-transplant CMP-FLECII,III . | Nine months post-transplant CMP-FLECIV . | ||
| Normal hepatocyte markers | H.2 | IIF | + | + | + | ||
| H.4 | IIF | + | + | + | |||
| H.1 | IIF | + | + | + | |||
| CYP450 2E1 | IIF | + | + | + | |||
| LAP | IIF | + | + | + | |||
| CEACAM 105 | IIF | + | + | + | |||
| DPPIV | IIF | + | + | + | |||
| Glu-6-Phos | Histo | + | + | + | |||
| ATPase | Histo | + | + | + | |||
| DPPIV | Histo | + | + | + | |||
| Oval/ductal markers | OC.10 | IIF | − | − | − | ||
| OC.2 | IIF | − | − | − | |||
| OC.4 | IIF | − | − | − | |||
| OC.5 | IIF | − | − | − | |||
| OV1 | IIF | − | − | − | |||
| OV6 | IIF | − | − | − | |||
| AFP | IIF | − | − | − | |||
| GGT | IIF | − | − | − | |||
| BD.2 | IIF | − | − | − | |||
| BD.1 | IIF | − | − | − | |||
| . | . | . | Phenotypes of donor cell colonies . | . | . | ||
|---|---|---|---|---|---|---|---|
| Reactivity . | Marker . | Assay . | Three months post-transplant CMP-FLECII,III,IV . | Six months post-transplant CMP-FLECII,III . | Nine months post-transplant CMP-FLECIV . | ||
| Normal hepatocyte markers | H.2 | IIF | + | + | + | ||
| H.4 | IIF | + | + | + | |||
| H.1 | IIF | + | + | + | |||
| CYP450 2E1 | IIF | + | + | + | |||
| LAP | IIF | + | + | + | |||
| CEACAM 105 | IIF | + | + | + | |||
| DPPIV | IIF | + | + | + | |||
| Glu-6-Phos | Histo | + | + | + | |||
| ATPase | Histo | + | + | + | |||
| DPPIV | Histo | + | + | + | |||
| Oval/ductal markers | OC.10 | IIF | − | − | − | ||
| OC.2 | IIF | − | − | − | |||
| OC.4 | IIF | − | − | − | |||
| OC.5 | IIF | − | − | − | |||
| OV1 | IIF | − | − | − | |||
| OV6 | IIF | − | − | − | |||
| AFP | IIF | − | − | − | |||
| GGT | IIF | − | − | − | |||
| BD.2 | IIF | − | − | − | |||
| BD.1 | IIF | − | − | − | |||
Isolates D, B and P were used for the 3-month time point; isolates D and R were used for the 6-month time point; isolate P was used for the 9-month time point.
IIF, indirect immunofluorescence; Histo, histochemical.