Details of primers used for RT-PCR analysis
| Gene . | Primers . | PCR conditions . | Polymorphism . | Gel conditions . | Cross . |
|---|---|---|---|---|---|
| Osbpl5 | F: CCACCATCCCAGATCAAGAC | 58°C | Ncol | 10% polyacrylamide | B6/Cast |
| R: TTCTCCTGTGTGGCTTTGTG | |||||
| Phlda2 | F: CGTGATGTCTTCGAAAACCG | 57°C | Deletion | 0.6×TBE, 0.5×MDE SSCP | B6/Cast |
| R: TGACGATGGTGAAGTACACG | |||||
| Cdkn1c (C) | F: GCCAAGCGCAAGAGAACT | 57°C | Taql | 1% agarose | B6/Cast |
| R: CAACAACCATTCCCCTAGAGTC | |||||
| Cdkn1c (S) | F: TTCAGATCTGACCTCAGACCC | 58°C | Aval | 10% polyacrylamide | B6/SD7 |
| R: GACCGGCTCAGTTCCCAGCTCAT | |||||
| Kcnq1ot1(C) | F: CTGAATTGGGGGAATAGCA | 57°C | Stul | 1% agarose | B6/Cast |
| R: CCATTTCTGCACCTGTTTT | |||||
| Kcnq1ot1(S) | F: TTGCCTGAGGATGGCTGTG | 57°C | Mwol | 1% agarose | B6/SD7 |
| R: CTTTCCGCTGTAACCTTTCTG | |||||
| Tssc4 | F: AGAAGCTGCCCATCCTGAGT | 59°C | Alul | 10% polyacrylamide | B6/Cast B6/SD7 |
| R: GACCCACAATTCCCACAGTC | |||||
| Cd81(C) | F: GCGTCCTTGCTTCAAAGAGA | 58°C | Faul | 1% agarose | B6/Cast |
| R: AGGCAAACAGGATCACAAGG | |||||
| Cd81(S) | F: GGGGACATGGCCTGTGTAT | 58°C | Deletion | 10% polyacrylamide | B6/SD7 |
| R: GCTCCCAAAAGGTAACAGGA | |||||
| Ascl2 | F: TGAGCATCCCACCCCCCTA | 55°C | Sfcl | 1% agarose | B6/Cast |
| R: CCAAACATCAGCGTCAGTATAG |
| Gene . | Primers . | PCR conditions . | Polymorphism . | Gel conditions . | Cross . |
|---|---|---|---|---|---|
| Osbpl5 | F: CCACCATCCCAGATCAAGAC | 58°C | Ncol | 10% polyacrylamide | B6/Cast |
| R: TTCTCCTGTGTGGCTTTGTG | |||||
| Phlda2 | F: CGTGATGTCTTCGAAAACCG | 57°C | Deletion | 0.6×TBE, 0.5×MDE SSCP | B6/Cast |
| R: TGACGATGGTGAAGTACACG | |||||
| Cdkn1c (C) | F: GCCAAGCGCAAGAGAACT | 57°C | Taql | 1% agarose | B6/Cast |
| R: CAACAACCATTCCCCTAGAGTC | |||||
| Cdkn1c (S) | F: TTCAGATCTGACCTCAGACCC | 58°C | Aval | 10% polyacrylamide | B6/SD7 |
| R: GACCGGCTCAGTTCCCAGCTCAT | |||||
| Kcnq1ot1(C) | F: CTGAATTGGGGGAATAGCA | 57°C | Stul | 1% agarose | B6/Cast |
| R: CCATTTCTGCACCTGTTTT | |||||
| Kcnq1ot1(S) | F: TTGCCTGAGGATGGCTGTG | 57°C | Mwol | 1% agarose | B6/SD7 |
| R: CTTTCCGCTGTAACCTTTCTG | |||||
| Tssc4 | F: AGAAGCTGCCCATCCTGAGT | 59°C | Alul | 10% polyacrylamide | B6/Cast B6/SD7 |
| R: GACCCACAATTCCCACAGTC | |||||
| Cd81(C) | F: GCGTCCTTGCTTCAAAGAGA | 58°C | Faul | 1% agarose | B6/Cast |
| R: AGGCAAACAGGATCACAAGG | |||||
| Cd81(S) | F: GGGGACATGGCCTGTGTAT | 58°C | Deletion | 10% polyacrylamide | B6/SD7 |
| R: GCTCCCAAAAGGTAACAGGA | |||||
| Ascl2 | F: TGAGCATCCCACCCCCCTA | 55°C | Sfcl | 1% agarose | B6/Cast |
| R: CCAAACATCAGCGTCAGTATAG |