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Table 2.

A role for MAK in midbrain hindbrain boundary formation

GroupGenenSame level, n (%)Reduced, n (%)Induced, n (%)Posterior shift, n (%)
MAK MO Otx2 58 16 (27) 41 (71) 1 (2)  
 En2 50 36 (72) 14 (28)  
 Gbx2 62 11 (18) 7 (11) 44 (71)  
CO MO Otx2 44 44 (96)  
 En2 49 48 (98) 1(2)  
 Gbx2 44 43 (98) 1 (2)  
Uninjected Otx2 30 30 (100)    
 En2 23 23 (100)    
 Gbx2 34 34 (100)    
MAK Otx2 47 15 (36) 6 (19) 10 (24) 16 (38) 
 En2 42 16 (39) 9 (22) 2 (5) 15 (37) 
 Gbx2 32 13 (40) 17(53) 2 (6) 
MAK KD Otx2 30 30 (100)    
 En2 21 21 (100)    
 Gbx2 30 28 (94) 1 (3) 1 (3)  
GroupGenenSame level, n (%)Reduced, n (%)Induced, n (%)Posterior shift, n (%)
MAK MO Otx2 58 16 (27) 41 (71) 1 (2)  
 En2 50 36 (72) 14 (28)  
 Gbx2 62 11 (18) 7 (11) 44 (71)  
CO MO Otx2 44 44 (96)  
 En2 49 48 (98) 1(2)  
 Gbx2 44 43 (98) 1 (2)  
Uninjected Otx2 30 30 (100)    
 En2 23 23 (100)    
 Gbx2 34 34 (100)    
MAK Otx2 47 15 (36) 6 (19) 10 (24) 16 (38) 
 En2 42 16 (39) 9 (22) 2 (5) 15 (37) 
 Gbx2 32 13 (40) 17(53) 2 (6) 
MAK KD Otx2 30 30 (100)    
 En2 21 21 (100)    
 Gbx2 30 28 (94) 1 (3) 1 (3)  

Embryos were injected with 50 ng of MOs or 2 ng of MAK RNAs into one dorsal animal blastomere at the four/eight-cell stage. At stage 19, embryos were .xed and expression of Otx2, En2 and Gbx2 were analyzed by in situ hybridization. Marker expression was assessed by comparing the injected and uninjected sides. n is number of scored embryos;percentage of affected embryos is shown in parentheses.

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