A role for MAK in midbrain hindbrain boundary formation
| Group . | Gene . | n . | Same level, n (%) . | Reduced, n (%) . | Induced, n (%) . | Posterior shift, n (%) . |
|---|---|---|---|---|---|---|
| MAK MO | Otx2 | 58 | 16 (27) | 41 (71) | 1 (2) | |
| En2 | 50 | 36 (72) | 14 (28) | 0 | ||
| Gbx2 | 62 | 11 (18) | 7 (11) | 44 (71) | ||
| CO MO | Otx2 | 44 | 44 (96) | 0 | 0 | |
| En2 | 49 | 48 (98) | 1(2) | 0 | ||
| Gbx2 | 44 | 43 (98) | 0 | 1 (2) | ||
| Uninjected | Otx2 | 30 | 30 (100) | |||
| En2 | 23 | 23 (100) | ||||
| Gbx2 | 34 | 34 (100) | ||||
| MAK | Otx2 | 47 | 15 (36) | 6 (19) | 10 (24) | 16 (38) |
| En2 | 42 | 16 (39) | 9 (22) | 2 (5) | 15 (37) | |
| Gbx2 | 32 | 13 (40) | 17(53) | 2 (6) | 0 | |
| MAK KD | Otx2 | 30 | 30 (100) | |||
| En2 | 21 | 21 (100) | ||||
| Gbx2 | 30 | 28 (94) | 1 (3) | 1 (3) |
| Group . | Gene . | n . | Same level, n (%) . | Reduced, n (%) . | Induced, n (%) . | Posterior shift, n (%) . |
|---|---|---|---|---|---|---|
| MAK MO | Otx2 | 58 | 16 (27) | 41 (71) | 1 (2) | |
| En2 | 50 | 36 (72) | 14 (28) | 0 | ||
| Gbx2 | 62 | 11 (18) | 7 (11) | 44 (71) | ||
| CO MO | Otx2 | 44 | 44 (96) | 0 | 0 | |
| En2 | 49 | 48 (98) | 1(2) | 0 | ||
| Gbx2 | 44 | 43 (98) | 0 | 1 (2) | ||
| Uninjected | Otx2 | 30 | 30 (100) | |||
| En2 | 23 | 23 (100) | ||||
| Gbx2 | 34 | 34 (100) | ||||
| MAK | Otx2 | 47 | 15 (36) | 6 (19) | 10 (24) | 16 (38) |
| En2 | 42 | 16 (39) | 9 (22) | 2 (5) | 15 (37) | |
| Gbx2 | 32 | 13 (40) | 17(53) | 2 (6) | 0 | |
| MAK KD | Otx2 | 30 | 30 (100) | |||
| En2 | 21 | 21 (100) | ||||
| Gbx2 | 30 | 28 (94) | 1 (3) | 1 (3) |
Embryos were injected with 50 ng of MOs or 2 ng of MAK RNAs into one dorsal animal blastomere at the four/eight-cell stage. At stage 19, embryos were .xed and expression of Otx2, En2 and Gbx2 were analyzed by in situ hybridization. Marker expression was assessed by comparing the injected and uninjected sides. n is number of scored embryos;percentage of affected embryos is shown in parentheses.