. | Mutants . | . | |
---|---|---|---|
Proteins . | unc-51(e369) . | unc-14(e57) . | |
In neurons | |||
UNC-5 (Netrin receptor) | +++ | + | |
UNC-40 (Netrin receptor) | − | − | |
UNC-44(ankyrin)* | − | ND | |
UNC-33 (CRMP)† | − | − | |
UNC-115 (abLIM) | − | − | |
UNC-76 (FEZ1) | − | − | |
CED-10 (Rac) | − | − | |
MIG-2 (Rac) | − | − | |
UNC-73 (Trio/GEF) | − | − | |
VAB-8 (kinesin) | − | − | |
LGG-1 (Atg8/LC3) | − | ND | |
RAB-11 (RAB 11) | − | ND | |
UNC-14 (RUN domain) | Weak‡ | ND | |
UNC-51 (S/T kinase) | ND | − | |
IDA-1 (ICA512)§ | − | − | |
SNB-1 (synaptobrevin)¶ | + | + | |
ODR-10 (odorant receptor) | − | − | |
GLR-1 (AMPA receptor) | − | − | |
In DTC or excretory cells | |||
UNC-5 (Netrin receptor) | − | − |
. | Mutants . | . | |
---|---|---|---|
Proteins . | unc-51(e369) . | unc-14(e57) . | |
In neurons | |||
UNC-5 (Netrin receptor) | +++ | + | |
UNC-40 (Netrin receptor) | − | − | |
UNC-44(ankyrin)* | − | ND | |
UNC-33 (CRMP)† | − | − | |
UNC-115 (abLIM) | − | − | |
UNC-76 (FEZ1) | − | − | |
CED-10 (Rac) | − | − | |
MIG-2 (Rac) | − | − | |
UNC-73 (Trio/GEF) | − | − | |
VAB-8 (kinesin) | − | − | |
LGG-1 (Atg8/LC3) | − | ND | |
RAB-11 (RAB 11) | − | ND | |
UNC-14 (RUN domain) | Weak‡ | ND | |
UNC-51 (S/T kinase) | ND | − | |
IDA-1 (ICA512)§ | − | − | |
SNB-1 (synaptobrevin)¶ | + | + | |
ODR-10 (odorant receptor) | − | − | |
GLR-1 (AMPA receptor) | − | − | |
In DTC or excretory cells | |||
UNC-5 (Netrin receptor) | − | − |
We performed these analyses using GFP-fusion proteins. In these assays, we examined whether or not the proteins accumulated in the cell bodies.
`+' indicates that we detected an abnormal accumulation in cell bodies compared with wild type.
Therefore, we did not examine them.
ND, not determined.
Personal communication from A. Otsuka.
Tsuboi et al. report that UNC-33 is accumulated at neural cell bodies in unc-51 and unc-14 mutants by using an anti-UNC-33 antibody staining (Tsuboi et al.,2005). We did not detect abnormal localization of UNC-33::GFP in DD/VD neurons of unc-51 and unc-14 mutants. This difference may be due to the different assay system.
Although UNC-14::GFP was easily detected in wild type, its expression was very weak in unc-51 mutants. UNC-14 may be unstable when UNC-51 is not present.
Report from Zahn et al. (Zahn et al.,2004).
Abnormally large varicosities imaged by SNB-I::GFP (a synaptic marker) were observed in ASI axons of unc-51 and unc-14 mutants(Crump et al., 2001). We found minor accumulations of SNB-1::GFP in cell bodies of DD/VD neurons of unc-51 and unc-14 mutants. We think that UNC-51 and UNC-14 regulate the localization of SNB-1 (cell bodies to axons) as well. We also tried examining UNC-34, RAB-5 and RAB-7 localization; however, these GFP fusion proteins accumulated at high levels in cell bodies of DD/VD neurons even in the wild-type background.