Effects of reagents on the area of organ culture
| . | Whole metanephroi . | . | . | Mesenchymal rudiments . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Reagent . | n . | Area at day 7 (mean ± s.d.) (mm2) . | P . | n . | Area at day 7 (mean ± s.d.) (mm2) . | P . | ||||
| Control (without reagents) | 7 | 1.717±0.381 | 6 | 1.912±0.200 | ||||||
| HIV-TAT peptide | 5 | 1.500±0.215 | 0.12 | 7 | 1.838±0.364 | 0.33 | ||||
| JNK inhibitor 1 | 6 | 1.020±0.325 | <0.005 | 7 | 0.651±0.131 | <0.001 | ||||
| JNK inhibitor 2 | 6 | 1.105±0.260 | <0.005 | 7 | 1.078±0.377 | <0.001 | ||||
| Y27,632 | 5 | 2.871±0.879 | <0.01 | 7 | 3.401±0.433 | <0.001 | ||||
| LiCl | 5 | 0.716±0.070 | <0.001 | 7 | 1.270±0.238 | <0.001 | ||||
| . | Whole metanephroi . | . | . | Mesenchymal rudiments . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Reagent . | n . | Area at day 7 (mean ± s.d.) (mm2) . | P . | n . | Area at day 7 (mean ± s.d.) (mm2) . | P . | ||||
| Control (without reagents) | 7 | 1.717±0.381 | 6 | 1.912±0.200 | ||||||
| HIV-TAT peptide | 5 | 1.500±0.215 | 0.12 | 7 | 1.838±0.364 | 0.33 | ||||
| JNK inhibitor 1 | 6 | 1.020±0.325 | <0.005 | 7 | 0.651±0.131 | <0.001 | ||||
| JNK inhibitor 2 | 6 | 1.105±0.260 | <0.005 | 7 | 1.078±0.377 | <0.001 | ||||
| Y27,632 | 5 | 2.871±0.879 | <0.01 | 7 | 3.401±0.433 | <0.001 | ||||
| LiCl | 5 | 0.716±0.070 | <0.001 | 7 | 1.270±0.238 | <0.001 | ||||
n, number of explants measured. P values were analyzed against control by using a t-test. Data from five independent experiments each for whole metanephroi and mesenchyme rudiments are shown.