Rescue experiments on the X-TSK-si/X-TSK MO phenotype
| Sample/probe . | Normal . | Expanded or ectopic . | Half-rescued . | Diminished . | |||
|---|---|---|---|---|---|---|---|
| X-TSK siRNA (X-TSK-si) 0.5 pmol+C-TSK 500 pg | |||||||
| Xslug | 18 | 8 | 8 | 5 (12.8%) | |||
| X-TSK siRNA (X-TSK-si) 0.5 pmol+X-TSK 250 pg | |||||||
| Sox9 | 9 | 2 | 7 | 5 (21.7%) | |||
| Xslug | 5 | 3 | 6 | 2 (12.5%) | |||
| X-TSK morpholino (X-TSK-MO) 5 ng+Ncad-X-TSK 500 pg | |||||||
| Sox9 | 14 | 0 | 6 | 1 (4.8%) | |||
| Xslug | 18 | 0 | 9 | 6 (18.2%) | |||
| X-TSK siRNA (X-TSK-si) 0.5 pmol+Chordin 100 pg | |||||||
| Sox9 | 3 | 0 | 19 | 0 | |||
| Xslug | 8 | 0 | 11 | 0 | |||
| Sample/probe . | Normal . | Expanded or ectopic . | Half-rescued . | Diminished . | |||
|---|---|---|---|---|---|---|---|
| X-TSK siRNA (X-TSK-si) 0.5 pmol+C-TSK 500 pg | |||||||
| Xslug | 18 | 8 | 8 | 5 (12.8%) | |||
| X-TSK siRNA (X-TSK-si) 0.5 pmol+X-TSK 250 pg | |||||||
| Sox9 | 9 | 2 | 7 | 5 (21.7%) | |||
| Xslug | 5 | 3 | 6 | 2 (12.5%) | |||
| X-TSK morpholino (X-TSK-MO) 5 ng+Ncad-X-TSK 500 pg | |||||||
| Sox9 | 14 | 0 | 6 | 1 (4.8%) | |||
| Xslug | 18 | 0 | 9 | 6 (18.2%) | |||
| X-TSK siRNA (X-TSK-si) 0.5 pmol+Chordin 100 pg | |||||||
| Sox9 | 3 | 0 | 19 | 0 | |||
| Xslug | 8 | 0 | 11 | 0 | |||
Compared with the phenotype of the X-TSK-si-injected embryo,embryos with restored expression by injection of a BMP antagonist were categorized as weakly rescued.