Cdx2-/- blastocysts plated on different substrata fail to attach and form a trophoblast outgrowth
| . | Cdx2genotype* . | . | . | ||
|---|---|---|---|---|---|
| ECM substrate . | +/+ . | +/- . | -/-† . | ||
| Fibronectin | 43/43 | 75/75 | 0/29(1) | ||
| Gelatin | 5/5 | 10/10 | 0/4(1) | ||
| Laminin | 12/12 | 13/13 | 0/7 | ||
| No substrate | 2/2 | 10/10 | 0/3 | ||
| . | Cdx2genotype* . | . | . | ||
|---|---|---|---|---|---|
| ECM substrate . | +/+ . | +/- . | -/-† . | ||
| Fibronectin | 43/43 | 75/75 | 0/29(1) | ||
| Gelatin | 5/5 | 10/10 | 0/4(1) | ||
| Laminin | 12/12 | 13/13 | 0/7 | ||
| No substrate | 2/2 | 10/10 | 0/3 | ||
Blastocysts from Cdx2+/- heterozygous intercrosses were plated in serum-containing medium on uncoated tissue culture dishes or pre-coated with fibronectin, gelatin or laminin (see Materials and methods).
For each substrate and genotype, the number of formed outgrowths/plated embryos is indicated
None of the Cdx2-/- embryos formed an outgrowth; number in parentheses indicates number of embryos showing attached parietal endoderm cells