Cell proliferation and death in vps33b morpholino-injected 4 dpf larvae
| . | Condition . | Number of sections (number of larvae) . | Number of cells examined . | % positive (± s.d.) . |
|---|---|---|---|---|
| PCNA | Wild type | 8 (6) | 692 | 93±7 |
| vps33b MO-injected | 9 (8) | 693 | 96±2 | |
| Apoptosis | Wild type | 5 (7) | 458 | 0 |
| vps33b MO-injected | 5 (8) | 432 | 0 |
| . | Condition . | Number of sections (number of larvae) . | Number of cells examined . | % positive (± s.d.) . |
|---|---|---|---|---|
| PCNA | Wild type | 8 (6) | 692 | 93±7 |
| vps33b MO-injected | 9 (8) | 693 | 96±2 | |
| Apoptosis | Wild type | 5 (7) | 458 | 0 |
| vps33b MO-injected | 5 (8) | 432 | 0 |
Control and vps33b-injected 4 dpf larvae were assayed for cell proliferation using the proliferating cell nuclear antigen (PCNA) antibody marker or for cell death using the Apoptag system. Representative sections through livers of multiple larvae were counted for stained nuclei,demonstrating proliferation (PCNA) or cell death (Apoptag). Representative larvae (one or two) were assayed in each section, which accounts for the discrepancy between the number of sections and number of larvae. The difference between wild-type and vps33b MO-injected PCNA larvae is not significant.