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Table 3.

Identity of the genes disrupted by Ds::KanR in female gametophytic mutants grouped by phenotypic category with the transmission ratios for each mutant gene

General defect observedMutant line IDDS element locationKanR:KanS crossed as femalePhenotype observed
In nuclear division phase of megagametogenesis EDA1 At1g59680 0.0092 Arrested at two-nuclear stage 
 EDA2 At2g18080* 0.0000 Arrested at two-nuclear stage 
 EDA3 At2g34860 0.0029 Arrested at two-nuclear stage 
 EDA4 At2g48140 0.0000 Arrested at four-nuclear stage 
 EDA 5 At3g03650 0.1900 Arrested at four-nuclear stage 
 EDA 6 At3g23440 0.3500 Arrested at two-nuclear stage 
 EDA 7 At3g56990 0.0090 Arrested at four-nuclear stage 
 EDA 8 At4g00310* 0.3300 Arrested at stage FG1 
 EDA 9 At4g34200 0.0000 Arrested at two-nuclear stage 
Abnormal nuclear numbers and positions EDA 10 At1g01960 0.4700 Variant nuclear number or not embryo sac 
 EDA 11 At1g55420 0.1700 Variant nuclear number and positions 
 EDA 12 At2g35950 0.5200 Variant nuclear number and positions 
 EDA 13 At2g47990 0.0000 Variant nuclear number and positions 
 EDA 14 At3g60360 0.0000 Abnormal positions and size four-nuclear stage 
 EDA 15 At4g14790 0.4400 Variant nuclear number and positions 
Arrested at varying stages of embryo sac development EDA 16 At1g61140 0.4600 Aberrant embryo sacs and with unfused polar nuclei 
 EDA 17 At1g72970 0.1800 Aberrant embryo sac and arrested at two-nuclear stage 
 EDA 18 At2g34920 0.3500 Aberrant embryo sacs and with unfused polar nuclei 
 EDA 19 At2g47990 0.0380 Aberrant embryo sacs and with unfused polar nuclei 
 EDA 20 At4g00020 0.2300 Aberrant embryo sacs and unfertilized ovules 
 EDA 21 At4g13235 0.4900 Aberrant embryo sacs and with unfused polar nuclei 
 EDA 22 At5g05920 0.3000 Arrested at four-nuclear stage and unfused polar nuclei 
 EDA 23 At5g44700 0.1974 Arrested at two-nuclear stage and unfused polar nuclei 
Unfused polar nuclei EDA 24 At1g70540 0.0208 Unfused polar nuclei 
 EDA 25 At1g72440 0.5846 Unfused polar nuclei 
 EDA 26 At2g01730 0.4100 Unfused polar nuclei 
 EDA 27 At2g20490 0.0000 Unfused polar nuclei 
 EDA 28 At2g34790 0.4100 Unfused polar nuclei 
 EDA 29 At2g35940 0.5100 Unfused polar nuclei 
 EDA 30 At3g03810 0.1200 Unfused polar nuclei 
 EDA 31 At3g10000 0.4600 Unfused polar nuclei 
 EDA 32 At3g62210 0.4773 Unfused polar nuclei 
 EDA 33 At4g00120 0.5200 Unfused polar nuclei 
 EDA 34 At4g00140 0.1573 Unfused polar nuclei 
 EDA 35 At4g05450 0.0350 Unfused polar nuclei 
 EDA 36 At4g13890 0.2500 Unfused polar nuclei 
 EDA 37 At4g13890 0.2500 Unfused polar nuclei 
 EDA 38 At4g14040 0.6840 Unfused polar nuclei 
 EDA 39 At4g33050 0.6200 Unfused polar nuclei 
 EDA 40 At4g37890 0.5844 Unfused polar nuclei 
 EDA 41 At5g52460 0.2900 Unfused polar nuclei 
Defects in fertilization UNE 1 At1g29300 0.2000 Defects in pollen tube attraction 
 UNE 2 At1g78130 0.2900 Unfertilized ovules but normal pollen tube attraction 
 UNE 3 At2g01110 0.7143 Unfertilized ovules but normal pollen tube attraction 
 UNE 4 At2g12940 0.3500 Defects in pollen tube attraction 
 UNE 5 At2g47470 0.7813 Unfertilized ovules but normal pollen tube attraction 
 UNE 6 At3g03340 0.8200 Unfertilized ovules but normal pollen tube attraction 
 UNE 7 At3g03690 0.2800 Unfertilized ovules but normal pollen tube attraction 
 UNE 8 At3g05690 0.1800 Unfertilized ovules but normal pollen tube attraction 
 UNE 9 At3g10560 0.3400 Defects in pollen tube attraction 
 UNE 10 At4g00050 0.4500 Unfertilized ovules but normal pollen tube attraction 
 UNE 11 At4g00080 0.6417 Defects in pollen tube attraction 
 UNE 12 At4g02590 0.5200 Unfertilized ovules but normal pollen tube attraction 
 UNE 13 At4g12620 0.3700 Unfertilized ovules but normal pollen tube attraction 
 UNE 14 At4g12860 0.5800 Defects in pollen tube attraction 
 UNE 15 At4g13560 0.4900 Defects in pollen tube attraction 
 UNE 16 At4g13640 0.2400 Unfertilized ovules but normal pollen tube attraction 
 UNE 17 At4g26330 0.2300 Unfertilized ovules but normal pollen tube attraction 
 UNE 18 At5g02100 0.1400 Unfertilized ovules but normal pollen tube attraction 
Arrested at one-cell zygotic stage MEE 1 ND 0.3800 Endosperm development arrested 
 MEE 2 ND 0.5600 Endosperm development arrested 
 MEE 3 At2g21650* 0.0610 Endosperm development arrested 
 MEE 4 At1g04630 0.0050 Endosperm development arrested 
 MEE 5 At1g06220 0.4100 Endosperm development arrested 
 MEE 6 At1g07890 0.0420 Endosperm development arrested 
 MEE 7 At1g10470 0.4400 Endosperm development arrested 
 MEE 8 At1g25310 0.1642 Endosperm development arrested 
 MEE 9 At1g60870 0.3100 Endosperm development arrested 
 MEE 10 At2g01200 0.5000 Endosperm development arrested 
 MEE 11 At2g01620 0.0000 Endosperm development arrested 
 MEE 12 At2g02955 0.4200 Endosperm development arrested 
 MEE 13 At2g14680 0.0000 Endosperm development arrested 
 MEE 14 At2g15890 0.0240 Endosperm development arrested 
 MEE 15 At2g16970 0.4300 Endosperm development arrested 
 MEE 16 At2g18650 0.0190 Endosperm normal 
 MEE 17 At2g22250 0.3333 Endosperm normal 
 MEE 18 At2g34090 0.2900 Endosperm development arrested 
 MEE 19 At2g34130 0.4800 Endosperm development arrested 
 MEE 20 At2g34220 0.1800 Endosperm development arrested 
 MEE 21 At2g34570 0.3800 Endosperm development arrested 
 MEE 22 At2g34780 0.4900 Endosperm development arrested 
 MEE 23 At2g34790 0.3913 Endosperm development arrested 
 MEE 24 At2g34830 0.2900 Endosperm development arrested 
 MEE 25 At2g34850 0.3000 Endosperm development arrested 
 MEE 26 At2g34870 0.4600 Endosperm development arrested 
 MEE 27 At2g34880 0.4800 Endosperm development arrested 
 MEE 28 At2g35210 0.4083 Endosperm development arrested 
 MEE 29 At2g35340 0.4100 Endosperm development arrested 
 MEE 30 At2g47470 0.2600 Endosperm development arrested 
 MEE 31 At3g02570 0.5300 Endosperm development arrested 
 MEE 32 At3g06350 0.5328 Endosperm development arrested 
 MEE 33 At3g10920 0.0556 Endosperm normal 
 MEE 34 At3g11270 0.2353 Endosperm normal 
 MEE 35 At3g15030 0.0837 Endosperm development arrested 
 MEE 36 At3g16440 0.0000 Endosperm development arrested 
 MEE 37 At3g23440 0.3000 Endosperm development arrested 
 MEE 38 At3g43160 0.4800 Endosperm development arrested 
 MEE 39 At3g46330 0.4700 Endosperm development arrested 
 MEE 40 At3g53700 0.4300 Endosperm development arrested 
 MEE 41 At3g62670 0.1800 Endosperm development arrested 
 MEE 42 At3g63080 0.5900 Endosperm development arrested 
 MEE 43 At4g00020 0.5100 Endosperm normal 
 MEE 44 At4g00060 0.2500 Endosperm development arrested 
 MEE 45 At4g00260 0.3519 Endosperm development arrested 
 MEE 46 At4g00310 0.2800 Endosperm development arrested 
 MEE 47 At4g00950 0.3400 Endosperm development arrested 
 MEE 48 At4g14080* 0.2200 Endosperm development arrested 
 MEE 49 At4g01560 0.1900 Endosperm development arrested 
 MEE 50 At4g00231 0.5700 Endosperm development arrested 
 MEE 51 At4g04040 0.1000 Endosperm development arrested 
 MEE 52 At4g04160 0.0435 Endosperm development arrested 
 MEE 53 At4g10560 0.1100 Endosperm normal 
 MEE 54 At4g11850 0.5500 Endosperm development arrested 
 MEE 55 At4g13345 0.4600 Endosperm development arrested 
 MEE 56 At4g13380 0.4300 Endosperm development arrested 
 MEE 57 At4g13610 0.5800 Endosperm development arrested 
 MEE 58 At4g13940 0.6593 Endosperm development arrested 
 MEE 59 At4g37300 0.3093 Endosperm development arrested 
 MEE 60 At5g05950 0.2500 Endosperm development arrested 
 MEE 61 At5g14220 0.4000 Endosperm development arrested 
 MEE 62 At5g45800 0.7714 Endosperm development arrested 
Embryo development defects MEE 63 At1g02140 0.8300 Embryos arrested at various stages of development 
 MEE 64 At1g79860 0.4500 Arrested at the two-cell embryo stage 
 MEE 65 At2g01280 0.6852 Arrested at early embryo stages 
 MEE 66 At2g02240 0.2000 Arrested at the two-cell embryo stage 
 MEE 67 At3g10110 0.3400 Arrested at the two-cell embryo stage 
 MEE 68 At4g24660 0.0972 Arrested at early embryo stages 
 MEE 69 At4g37140 0.2300 Arrested at the two-cell embryo stage 
 MEE 70 At5g58230 0.0000 Abnormal embryo development 
General defect observedMutant line IDDS element locationKanR:KanS crossed as femalePhenotype observed
In nuclear division phase of megagametogenesis EDA1 At1g59680 0.0092 Arrested at two-nuclear stage 
 EDA2 At2g18080* 0.0000 Arrested at two-nuclear stage 
 EDA3 At2g34860 0.0029 Arrested at two-nuclear stage 
 EDA4 At2g48140 0.0000 Arrested at four-nuclear stage 
 EDA 5 At3g03650 0.1900 Arrested at four-nuclear stage 
 EDA 6 At3g23440 0.3500 Arrested at two-nuclear stage 
 EDA 7 At3g56990 0.0090 Arrested at four-nuclear stage 
 EDA 8 At4g00310* 0.3300 Arrested at stage FG1 
 EDA 9 At4g34200 0.0000 Arrested at two-nuclear stage 
Abnormal nuclear numbers and positions EDA 10 At1g01960 0.4700 Variant nuclear number or not embryo sac 
 EDA 11 At1g55420 0.1700 Variant nuclear number and positions 
 EDA 12 At2g35950 0.5200 Variant nuclear number and positions 
 EDA 13 At2g47990 0.0000 Variant nuclear number and positions 
 EDA 14 At3g60360 0.0000 Abnormal positions and size four-nuclear stage 
 EDA 15 At4g14790 0.4400 Variant nuclear number and positions 
Arrested at varying stages of embryo sac development EDA 16 At1g61140 0.4600 Aberrant embryo sacs and with unfused polar nuclei 
 EDA 17 At1g72970 0.1800 Aberrant embryo sac and arrested at two-nuclear stage 
 EDA 18 At2g34920 0.3500 Aberrant embryo sacs and with unfused polar nuclei 
 EDA 19 At2g47990 0.0380 Aberrant embryo sacs and with unfused polar nuclei 
 EDA 20 At4g00020 0.2300 Aberrant embryo sacs and unfertilized ovules 
 EDA 21 At4g13235 0.4900 Aberrant embryo sacs and with unfused polar nuclei 
 EDA 22 At5g05920 0.3000 Arrested at four-nuclear stage and unfused polar nuclei 
 EDA 23 At5g44700 0.1974 Arrested at two-nuclear stage and unfused polar nuclei 
Unfused polar nuclei EDA 24 At1g70540 0.0208 Unfused polar nuclei 
 EDA 25 At1g72440 0.5846 Unfused polar nuclei 
 EDA 26 At2g01730 0.4100 Unfused polar nuclei 
 EDA 27 At2g20490 0.0000 Unfused polar nuclei 
 EDA 28 At2g34790 0.4100 Unfused polar nuclei 
 EDA 29 At2g35940 0.5100 Unfused polar nuclei 
 EDA 30 At3g03810 0.1200 Unfused polar nuclei 
 EDA 31 At3g10000 0.4600 Unfused polar nuclei 
 EDA 32 At3g62210 0.4773 Unfused polar nuclei 
 EDA 33 At4g00120 0.5200 Unfused polar nuclei 
 EDA 34 At4g00140 0.1573 Unfused polar nuclei 
 EDA 35 At4g05450 0.0350 Unfused polar nuclei 
 EDA 36 At4g13890 0.2500 Unfused polar nuclei 
 EDA 37 At4g13890 0.2500 Unfused polar nuclei 
 EDA 38 At4g14040 0.6840 Unfused polar nuclei 
 EDA 39 At4g33050 0.6200 Unfused polar nuclei 
 EDA 40 At4g37890 0.5844 Unfused polar nuclei 
 EDA 41 At5g52460 0.2900 Unfused polar nuclei 
Defects in fertilization UNE 1 At1g29300 0.2000 Defects in pollen tube attraction 
 UNE 2 At1g78130 0.2900 Unfertilized ovules but normal pollen tube attraction 
 UNE 3 At2g01110 0.7143 Unfertilized ovules but normal pollen tube attraction 
 UNE 4 At2g12940 0.3500 Defects in pollen tube attraction 
 UNE 5 At2g47470 0.7813 Unfertilized ovules but normal pollen tube attraction 
 UNE 6 At3g03340 0.8200 Unfertilized ovules but normal pollen tube attraction 
 UNE 7 At3g03690 0.2800 Unfertilized ovules but normal pollen tube attraction 
 UNE 8 At3g05690 0.1800 Unfertilized ovules but normal pollen tube attraction 
 UNE 9 At3g10560 0.3400 Defects in pollen tube attraction 
 UNE 10 At4g00050 0.4500 Unfertilized ovules but normal pollen tube attraction 
 UNE 11 At4g00080 0.6417 Defects in pollen tube attraction 
 UNE 12 At4g02590 0.5200 Unfertilized ovules but normal pollen tube attraction 
 UNE 13 At4g12620 0.3700 Unfertilized ovules but normal pollen tube attraction 
 UNE 14 At4g12860 0.5800 Defects in pollen tube attraction 
 UNE 15 At4g13560 0.4900 Defects in pollen tube attraction 
 UNE 16 At4g13640 0.2400 Unfertilized ovules but normal pollen tube attraction 
 UNE 17 At4g26330 0.2300 Unfertilized ovules but normal pollen tube attraction 
 UNE 18 At5g02100 0.1400 Unfertilized ovules but normal pollen tube attraction 
Arrested at one-cell zygotic stage MEE 1 ND 0.3800 Endosperm development arrested 
 MEE 2 ND 0.5600 Endosperm development arrested 
 MEE 3 At2g21650* 0.0610 Endosperm development arrested 
 MEE 4 At1g04630 0.0050 Endosperm development arrested 
 MEE 5 At1g06220 0.4100 Endosperm development arrested 
 MEE 6 At1g07890 0.0420 Endosperm development arrested 
 MEE 7 At1g10470 0.4400 Endosperm development arrested 
 MEE 8 At1g25310 0.1642 Endosperm development arrested 
 MEE 9 At1g60870 0.3100 Endosperm development arrested 
 MEE 10 At2g01200 0.5000 Endosperm development arrested 
 MEE 11 At2g01620 0.0000 Endosperm development arrested 
 MEE 12 At2g02955 0.4200 Endosperm development arrested 
 MEE 13 At2g14680 0.0000 Endosperm development arrested 
 MEE 14 At2g15890 0.0240 Endosperm development arrested 
 MEE 15 At2g16970 0.4300 Endosperm development arrested 
 MEE 16 At2g18650 0.0190 Endosperm normal 
 MEE 17 At2g22250 0.3333 Endosperm normal 
 MEE 18 At2g34090 0.2900 Endosperm development arrested 
 MEE 19 At2g34130 0.4800 Endosperm development arrested 
 MEE 20 At2g34220 0.1800 Endosperm development arrested 
 MEE 21 At2g34570 0.3800 Endosperm development arrested 
 MEE 22 At2g34780 0.4900 Endosperm development arrested 
 MEE 23 At2g34790 0.3913 Endosperm development arrested 
 MEE 24 At2g34830 0.2900 Endosperm development arrested 
 MEE 25 At2g34850 0.3000 Endosperm development arrested 
 MEE 26 At2g34870 0.4600 Endosperm development arrested 
 MEE 27 At2g34880 0.4800 Endosperm development arrested 
 MEE 28 At2g35210 0.4083 Endosperm development arrested 
 MEE 29 At2g35340 0.4100 Endosperm development arrested 
 MEE 30 At2g47470 0.2600 Endosperm development arrested 
 MEE 31 At3g02570 0.5300 Endosperm development arrested 
 MEE 32 At3g06350 0.5328 Endosperm development arrested 
 MEE 33 At3g10920 0.0556 Endosperm normal 
 MEE 34 At3g11270 0.2353 Endosperm normal 
 MEE 35 At3g15030 0.0837 Endosperm development arrested 
 MEE 36 At3g16440 0.0000 Endosperm development arrested 
 MEE 37 At3g23440 0.3000 Endosperm development arrested 
 MEE 38 At3g43160 0.4800 Endosperm development arrested 
 MEE 39 At3g46330 0.4700 Endosperm development arrested 
 MEE 40 At3g53700 0.4300 Endosperm development arrested 
 MEE 41 At3g62670 0.1800 Endosperm development arrested 
 MEE 42 At3g63080 0.5900 Endosperm development arrested 
 MEE 43 At4g00020 0.5100 Endosperm normal 
 MEE 44 At4g00060 0.2500 Endosperm development arrested 
 MEE 45 At4g00260 0.3519 Endosperm development arrested 
 MEE 46 At4g00310 0.2800 Endosperm development arrested 
 MEE 47 At4g00950 0.3400 Endosperm development arrested 
 MEE 48 At4g14080* 0.2200 Endosperm development arrested 
 MEE 49 At4g01560 0.1900 Endosperm development arrested 
 MEE 50 At4g00231 0.5700 Endosperm development arrested 
 MEE 51 At4g04040 0.1000 Endosperm development arrested 
 MEE 52 At4g04160 0.0435 Endosperm development arrested 
 MEE 53 At4g10560 0.1100 Endosperm normal 
 MEE 54 At4g11850 0.5500 Endosperm development arrested 
 MEE 55 At4g13345 0.4600 Endosperm development arrested 
 MEE 56 At4g13380 0.4300 Endosperm development arrested 
 MEE 57 At4g13610 0.5800 Endosperm development arrested 
 MEE 58 At4g13940 0.6593 Endosperm development arrested 
 MEE 59 At4g37300 0.3093 Endosperm development arrested 
 MEE 60 At5g05950 0.2500 Endosperm development arrested 
 MEE 61 At5g14220 0.4000 Endosperm development arrested 
 MEE 62 At5g45800 0.7714 Endosperm development arrested 
Embryo development defects MEE 63 At1g02140 0.8300 Embryos arrested at various stages of development 
 MEE 64 At1g79860 0.4500 Arrested at the two-cell embryo stage 
 MEE 65 At2g01280 0.6852 Arrested at early embryo stages 
 MEE 66 At2g02240 0.2000 Arrested at the two-cell embryo stage 
 MEE 67 At3g10110 0.3400 Arrested at the two-cell embryo stage 
 MEE 68 At4g24660 0.0972 Arrested at early embryo stages 
 MEE 69 At4g37140 0.2300 Arrested at the two-cell embryo stage 
 MEE 70 At5g58230 0.0000 Abnormal embryo development 

ND, not determined.

*

Indicates sequences identified by TAIL-PCR but not confirmed yet by gene-specific primers.

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