PCR primers and cycling conditions
| Primers . | Origin . | Sequence . | Denaturing temp . | Annealing temperature (°C)/time (seconds) . | Extension temperature (°C)/time (seconds) . | Acquisition temperature (°C)/time (seconds) . |
|---|---|---|---|---|---|---|
| Cardiac actin | XMMR* | U: 5-TCC CTG TAC GCT TCT GGT CGT A -3 | 95 | 58/5 | 72/12 | 83/3 |
| D: 5-TCT CAA AGT CCA AAG CCA CAT A-3 | ||||||
| Chordin | XMMR* | U: 5-AAC TGC CAG GAC TGG ATG GT-3 | 95 | 55/5 | 72/12 | 81/3 |
| D: 5-GGC AGG ATT TAG AGT TGC TTC-3 | ||||||
| Derrière | Kofron et al., 1999 | U: 5-TGG CAG AGT TGT GGC TAT CA-3 | 95 | 55/5 | 72/18 | 82/3 |
| D: 5-CTA TGG CTG CTA TGG TTC CTT-3 | ||||||
| FGF8 | Kofron et al., 1999 | U: 5-CTG GTG ACC GAC CAA CTA AG-3 | 95 | 55/5 | 72/14 | 86/3 |
| D: 5-ACC AGC CTT CGT ACT TGA CA-3 | ||||||
| Furin | New | U: 5-TGTAGCCTCCAGCCAGTCTT-3 | 95 | 58/5 | 72/9 | 82/3 |
| D: 5-GGCAGTCATTGATTGTGGTG-3 | ||||||
| Goosecoid v.2 | Kofron et al. 2004 | U: 5′-TTC ACC GAT GAA CAA CTG GA-3′ | 95 | 55/5 | 72/11 | 82/3 |
| D: 5′-TTC CAC TTT TGG GCA TTT TC-3′ | ||||||
| MyoDa | XMMR* | U: 5-AGC TCC AAC TGC TCC GAC GGC ATG AA-3 | 95 | 55/5 | 72/18 | 86/3 |
| D: 5-AGG AGA GAA TCC AGT TGA TGG AAA CA-3 | ||||||
| ODC | Heasman et al., 2000 | U: 5-GCC ATT GTG AAG ACT CTC TCC ATT C-3 | 95 | 55/5 | 72/12 | 83/3 |
| D: 5-TTC GGG TGA TTC CTT GCC AC-3 | ||||||
| XPACE4 | New | U: 5-TGTCTCCTAGCGGGATGACT-3 | 95 | 55/5 | 72/10 | 84/3 |
| D: 5-CTGGCGCAGAAAATGTAGGT-3 | ||||||
| N-CAM | Yokota et al., 2003 | U: 5-CAC AGT TCC ACC AAA TGC-3 | 95 | 60/5 | 72/13 | 84/3 |
| D: 5-GGA ATC AAG CGG TAC AGA -3 | ||||||
| Vg1 | New | U: 5-CCATACCCGCTGACAGAAAT-3 | 95 | 58/5 | 72/8 | 80/3 |
| D: 5-CCATACCCGCTGACAGAAAT-3 | ||||||
| Xbra | Sun et al., 1999 | U: 5-TTC TGA AGG TGA GCA TGT CG-3 | 95 | 55/5 | 72/8 | 75/3 |
| D: 5-GTT TGA CTT TGC TAA AAG AGA CAG G-3 | ||||||
| Xnr1 | Kofron et al., 1999 | U: 5-TGG CCA GAT AGA GTA GAG-3 | 95 | 55/5 | 72/12 | 81/3 |
| D: 5-TCC AAC GGT TCT CAC TTT-3 | ||||||
| Xnr-3 | Kofron et al., 1999 | U: 5-CTT CTG CAC TAG ATT CTG-3 | 95 | 57/5 | 72/10 | 79/3 |
| D: 5-CAG CTT CTG GCC AAG ACT-3 | ||||||
| XSox17α | Xanthos et. al. 2001 | U: 5-GCA AGA TGC TTG GCA AGT CG-3 | 95 | 58/5 | 72/8 | 85/3 |
| D: 5-GCT GAA GTT CTC TAG ACA CA-3 |
| Primers . | Origin . | Sequence . | Denaturing temp . | Annealing temperature (°C)/time (seconds) . | Extension temperature (°C)/time (seconds) . | Acquisition temperature (°C)/time (seconds) . |
|---|---|---|---|---|---|---|
| Cardiac actin | XMMR* | U: 5-TCC CTG TAC GCT TCT GGT CGT A -3 | 95 | 58/5 | 72/12 | 83/3 |
| D: 5-TCT CAA AGT CCA AAG CCA CAT A-3 | ||||||
| Chordin | XMMR* | U: 5-AAC TGC CAG GAC TGG ATG GT-3 | 95 | 55/5 | 72/12 | 81/3 |
| D: 5-GGC AGG ATT TAG AGT TGC TTC-3 | ||||||
| Derrière | Kofron et al., 1999 | U: 5-TGG CAG AGT TGT GGC TAT CA-3 | 95 | 55/5 | 72/18 | 82/3 |
| D: 5-CTA TGG CTG CTA TGG TTC CTT-3 | ||||||
| FGF8 | Kofron et al., 1999 | U: 5-CTG GTG ACC GAC CAA CTA AG-3 | 95 | 55/5 | 72/14 | 86/3 |
| D: 5-ACC AGC CTT CGT ACT TGA CA-3 | ||||||
| Furin | New | U: 5-TGTAGCCTCCAGCCAGTCTT-3 | 95 | 58/5 | 72/9 | 82/3 |
| D: 5-GGCAGTCATTGATTGTGGTG-3 | ||||||
| Goosecoid v.2 | Kofron et al. 2004 | U: 5′-TTC ACC GAT GAA CAA CTG GA-3′ | 95 | 55/5 | 72/11 | 82/3 |
| D: 5′-TTC CAC TTT TGG GCA TTT TC-3′ | ||||||
| MyoDa | XMMR* | U: 5-AGC TCC AAC TGC TCC GAC GGC ATG AA-3 | 95 | 55/5 | 72/18 | 86/3 |
| D: 5-AGG AGA GAA TCC AGT TGA TGG AAA CA-3 | ||||||
| ODC | Heasman et al., 2000 | U: 5-GCC ATT GTG AAG ACT CTC TCC ATT C-3 | 95 | 55/5 | 72/12 | 83/3 |
| D: 5-TTC GGG TGA TTC CTT GCC AC-3 | ||||||
| XPACE4 | New | U: 5-TGTCTCCTAGCGGGATGACT-3 | 95 | 55/5 | 72/10 | 84/3 |
| D: 5-CTGGCGCAGAAAATGTAGGT-3 | ||||||
| N-CAM | Yokota et al., 2003 | U: 5-CAC AGT TCC ACC AAA TGC-3 | 95 | 60/5 | 72/13 | 84/3 |
| D: 5-GGA ATC AAG CGG TAC AGA -3 | ||||||
| Vg1 | New | U: 5-CCATACCCGCTGACAGAAAT-3 | 95 | 58/5 | 72/8 | 80/3 |
| D: 5-CCATACCCGCTGACAGAAAT-3 | ||||||
| Xbra | Sun et al., 1999 | U: 5-TTC TGA AGG TGA GCA TGT CG-3 | 95 | 55/5 | 72/8 | 75/3 |
| D: 5-GTT TGA CTT TGC TAA AAG AGA CAG G-3 | ||||||
| Xnr1 | Kofron et al., 1999 | U: 5-TGG CCA GAT AGA GTA GAG-3 | 95 | 55/5 | 72/12 | 81/3 |
| D: 5-TCC AAC GGT TCT CAC TTT-3 | ||||||
| Xnr-3 | Kofron et al., 1999 | U: 5-CTT CTG CAC TAG ATT CTG-3 | 95 | 57/5 | 72/10 | 79/3 |
| D: 5-CAG CTT CTG GCC AAG ACT-3 | ||||||
| XSox17α | Xanthos et. al. 2001 | U: 5-GCA AGA TGC TTG GCA AGT CG-3 | 95 | 58/5 | 72/8 | 85/3 |
| D: 5-GCT GAA GTT CTC TAG ACA CA-3 |
Xenopus molecular marker resource http://www.xenbase.org/xmmr/Marker_pages/primers.html