Rescue of cell migration defects
| Genotype . | Transgene expression . | % Defective HSN . | n . |
|---|---|---|---|
| A. HSN migration | |||
| Wild type | N/A | 0 | 50 |
| sdn-1(zh20) | N/A | 55±3 | 52 |
| sdn-1(zh20); opEx1216[Psdn-1::sdn-1] | Hypodermis, neurons | 13±2 | 62 |
| sdn-1(zh20); opEx1217[Psdn-1::sdn-1] | Hypodermis, neurons | 13±3 | 46 |
| sdn-1(zh20); opEx1218[Psdn-1::sdn-1] | Hypodermis, neurons | 14±2 | 77 |
| sdn-1(zh20); opEx1221[Punc-119::sdn-1] | Pan-neuronal | 4±1 | 75 |
| sdn-1(zh20); opEx1222[Punc-119::sdn-1] | Pan-neuronal | 3±1 | 68 |
| sdn-1(zh20); opEx1224[Punc-119::sdn-1] | Pan-neuronal | 7±2 | 82 |
| sdn-1(zh20); opEx1226[Pdpy-7::sdn-1] | Hypodermis | 40±3 | 78 |
| sdn-1(zh20); opEx1227[Pdpy-7::sdn-1] | Hypodermis | 35±2 | 130 |
| sdn-1(zh20); opEx1230[Pdpy-7::sdn-1] | Hypodermis | 31±3 | 64 |
| Genotype . | Transgene expression . | % Defective HSN . | n . |
|---|---|---|---|
| A. HSN migration | |||
| Wild type | N/A | 0 | 50 |
| sdn-1(zh20) | N/A | 55±3 | 52 |
| sdn-1(zh20); opEx1216[Psdn-1::sdn-1] | Hypodermis, neurons | 13±2 | 62 |
| sdn-1(zh20); opEx1217[Psdn-1::sdn-1] | Hypodermis, neurons | 13±3 | 46 |
| sdn-1(zh20); opEx1218[Psdn-1::sdn-1] | Hypodermis, neurons | 14±2 | 77 |
| sdn-1(zh20); opEx1221[Punc-119::sdn-1] | Pan-neuronal | 4±1 | 75 |
| sdn-1(zh20); opEx1222[Punc-119::sdn-1] | Pan-neuronal | 3±1 | 68 |
| sdn-1(zh20); opEx1224[Punc-119::sdn-1] | Pan-neuronal | 7±2 | 82 |
| sdn-1(zh20); opEx1226[Pdpy-7::sdn-1] | Hypodermis | 40±3 | 78 |
| sdn-1(zh20); opEx1227[Pdpy-7::sdn-1] | Hypodermis | 35±2 | 130 |
| sdn-1(zh20); opEx1230[Pdpy-7::sdn-1] | Hypodermis | 31±3 | 64 |
| Genotype . | Transgene expression . | % Defective ALM . | n . |
|---|---|---|---|
| B. ALM migration | |||
| Wild type | N/A | 0±1 | 100 |
| sdn-1(zh20) | N/A | 42±3 | 96 |
| sdn-1(zh20); opEx1196[Psdn-1::sdn-1] | Hypodermis, neurons | 19±2 | 68 |
| sdn-1(zh20); opEx1198[Psdn-1::sdn-1] | Hypodermis, neurons | 10±2 | 69 |
| sdn-1(zh20); opEx1199[Psdn-1::sdn-1] | Hypodermis, neurons | 13±2 | 85 |
| sdn-1(zh20); opEx1206[Punc-119::sdn-1] | Pan-neuronal | 5±1 | 82 |
| sdn-1(zh20); opEx1207[Punc-119::sdn-1] | Pan-neuronal | 9±2 | 80 |
| sdn-1(zh20); opEx1208[Punc-119::sdn-1] | Pan-neuronal | 6±1 | 82 |
| sdn-1(zh20); opEx1202[Pdpy-7::sdn-1] | Hypodermis | 51±4 | 43 |
| sdn-1(zh20); opEx1204[Pdpy-7::sdn-1] | Hypodermis | 51±3 | 63 |
| sdn-1(zh20); opEx1205[Pdpy-7::sdn-1] | Hypodermis | 32±3 | 50 |
| Genotype . | Transgene expression . | % Defective ALM . | n . |
|---|---|---|---|
| B. ALM migration | |||
| Wild type | N/A | 0±1 | 100 |
| sdn-1(zh20) | N/A | 42±3 | 96 |
| sdn-1(zh20); opEx1196[Psdn-1::sdn-1] | Hypodermis, neurons | 19±2 | 68 |
| sdn-1(zh20); opEx1198[Psdn-1::sdn-1] | Hypodermis, neurons | 10±2 | 69 |
| sdn-1(zh20); opEx1199[Psdn-1::sdn-1] | Hypodermis, neurons | 13±2 | 85 |
| sdn-1(zh20); opEx1206[Punc-119::sdn-1] | Pan-neuronal | 5±1 | 82 |
| sdn-1(zh20); opEx1207[Punc-119::sdn-1] | Pan-neuronal | 9±2 | 80 |
| sdn-1(zh20); opEx1208[Punc-119::sdn-1] | Pan-neuronal | 6±1 | 82 |
| sdn-1(zh20); opEx1202[Pdpy-7::sdn-1] | Hypodermis | 51±4 | 43 |
| sdn-1(zh20); opEx1204[Pdpy-7::sdn-1] | Hypodermis | 51±3 | 63 |
| sdn-1(zh20); opEx1205[Pdpy-7::sdn-1] | Hypodermis | 32±3 | 50 |