Component . | Dishevelled domain bound . | Binding assay† . | Role . | Cellular localization . | Pathway function . | Reference . |
---|---|---|---|---|---|---|
Dishevelled | DIX | Yeast two hybrid | Agonist | Cytoplasmic | GOF: canonical/PCP/Ca2+ | Kishida et al., 1999; Rothbacher et al., 2000 |
IP | Membrane | LOF: canonical/PCP | ||||
GST-pulldown | Nuclear | DN: canonical/PCP/ Ca2+ | ||||
PAK1 | DIX | IP | Agonist | Cytoplasmic | AchR clustering | Luo et al., 2002 |
GST-pulldown | Membrane | |||||
Axin | DIX | Yeast two hybrid | Antagonist | Cytoplasmic | GOF: canonical | Itoh et al., 2000; Julius et al., 2000; Kishida et al., 1999; Zeng et al., 1997 |
PDZ | IP | Membrane | LOF: canonical | |||
GST-pulldown | Nuclear | DN: canonical | ||||
Frizzled | PDZ | Yeast two hybrid | Agonist | Membrane | GOF: canonical/PCP/Ca2+ | Cong et al., 2004; Wong et al.,2003 |
IP | LOF: canonical/PCP | |||||
GST-pulldown | DN: canonical/PCP | |||||
Casein Kinase 1 | PDZ | IP | Agonist | Cytoplasmic | GOF: canonical | Peters et al., 1999; Sakanaka et al., 1999 |
GST-pulldown | Nuclear | LOF: canonical | ||||
DN: canonical | ||||||
Casein Kinase 2 | PDZ | Biochemical purification | Agonist | Cytoplasmic | GOF: canonical | Willert et al., 1997 |
IP | Nuclear | |||||
GST-pulldown | ||||||
GSK3-binding protein/FRAT | PDZ | Yeast two hybrid | Agonist | Cytoplasmic | GOF: canonical | Li et al., 1999; Yost et al.,1998 |
IP | LOF: canonical | |||||
Frodo | PDZ | Yeast two hybrid | Agonist | Unknown | GOF: canonical | Gloy et al., 2002 |
IP | LOF: canonical | |||||
DN: canonical | ||||||
Dapper | PDZ | Yeast two hybrid | Antagonist | Cytoplasmic | GOF: canonical | Cheyette et al., 2002 |
IP‡ | Nuclear? | LOF: canonical/PCP | ||||
GST-pulldown | DN: canonical | |||||
IDAX | PDZ | Yeast two hybrid | Antagonist | Cytoplasmic | GOF: canonical | Hino et al., 2001 |
IP | LOF: canonical | |||||
GST-pulldown | DN: canonical | |||||
Naked Cuticle | PDZ | Yeast two hybrid | Antagonist | Cytoplasmic | GOF: canonical/PCP | Rousset et al., 2001; Wharton et al., 2001 |
IP | LOF: canonical | |||||
DN: canonical | ||||||
Strabismus | PDZ | IP | Agonist/Antagonist | Membrane | GOF: PCP | Bastock et al., 2003; Jessen et al., 2002; Park and Moon, 2002; Wolff and Rubin, 1998 |
GST-pulldown | LOF: PCP | |||||
DN: PCP | ||||||
PAR1 | PDZ | Biochemical purification | Agonist/antagonist | Cytoplasmic? | PAR1BY GOF: PCP | Ossipova et al., 2005; Sun et al., 2001 |
PAR1BY LOF: PCP | ||||||
IP | PAR1A/BX GOF: canonical | |||||
PAR1A/BX LOF: canonical | ||||||
Diego/Diversin | PDZ | IP | Agonist/Antagonist | Cytoplasmic | GOF: canonical + PCP | Jenny et al., 2005 |
LOF: PCP | ||||||
Protein Phosphatase 2C | PDZ | IP | Agonist | Cytoplasmic | GOF: canonical | Ratcliffe et al., 2000; Strovel et al., 2000; Yang et al., 2003 |
Notch | PDZ | Yeast two hybrid | ND | ND | ND | Axelrod et al., 1996 |
IP | ||||||
EPS8 | PDZ | IP | Unknown | Cytoplasmic? | Unknown | Inobe et al., 1999 |
Yeast two hybrid | ||||||
Daple | PDZ | Yeast two hybrid | Agonist | Unknown | GOF: canonical + PCP | Oshita et al., 2003 |
IP | ||||||
Pulldown assay | ||||||
DAAM1 | PDZ | Yeast two hybrid | Agonist | Cytoplasmic | GOF: PCP | R.H., unpublished |
DEP | IP‡ | Membrane | LOF: PCP | |||
GST-pulldown | DN: PCP | |||||
Prickle | DEP | IP | Agonist | Cytoplasmic | GOF: PCP | Jenny et al., 2005; Tree et al., 2002 |
GST-pulldown | Membrane | LOF: PCP | ||||
DN: PCP | ||||||
MuSK | DEP | Yeast two hybrid | Agonist | Cytoplasmic | Acetylcholine receptor clustering | Luo et al., 2002 |
IP‡ | Membrane | |||||
GST-pulldown | ||||||
Disabled 2 | DEP | IP | Antagonist | Membrane | GOF: canonical | Hocevar et al., 2003 |
LOF: canonical | ||||||
EphrinB1 | Full length | IP | Agonist? | Membrane | GOF: PCP? | Tanaka et al., 2003 |
GST-pulldown | ||||||
PKCδ | Full length | IP | Agonist | Cytoplasmic | LOF: PCP | Kinoshita et al., 2003 |
Membrane | DN: PCP | |||||
NET1 | Full length | IP | Antagonist? | Cytoplasmic | GOF: PCP | Miyakoshi et al., 2004 |
β-Arrestin | Full length | IP | Unknown | Cytoplasmic | GOF: receptor internalization | Chen et al., 2003 |
Component . | Dishevelled domain bound . | Binding assay† . | Role . | Cellular localization . | Pathway function . | Reference . |
---|---|---|---|---|---|---|
Dishevelled | DIX | Yeast two hybrid | Agonist | Cytoplasmic | GOF: canonical/PCP/Ca2+ | Kishida et al., 1999; Rothbacher et al., 2000 |
IP | Membrane | LOF: canonical/PCP | ||||
GST-pulldown | Nuclear | DN: canonical/PCP/ Ca2+ | ||||
PAK1 | DIX | IP | Agonist | Cytoplasmic | AchR clustering | Luo et al., 2002 |
GST-pulldown | Membrane | |||||
Axin | DIX | Yeast two hybrid | Antagonist | Cytoplasmic | GOF: canonical | Itoh et al., 2000; Julius et al., 2000; Kishida et al., 1999; Zeng et al., 1997 |
PDZ | IP | Membrane | LOF: canonical | |||
GST-pulldown | Nuclear | DN: canonical | ||||
Frizzled | PDZ | Yeast two hybrid | Agonist | Membrane | GOF: canonical/PCP/Ca2+ | Cong et al., 2004; Wong et al.,2003 |
IP | LOF: canonical/PCP | |||||
GST-pulldown | DN: canonical/PCP | |||||
Casein Kinase 1 | PDZ | IP | Agonist | Cytoplasmic | GOF: canonical | Peters et al., 1999; Sakanaka et al., 1999 |
GST-pulldown | Nuclear | LOF: canonical | ||||
DN: canonical | ||||||
Casein Kinase 2 | PDZ | Biochemical purification | Agonist | Cytoplasmic | GOF: canonical | Willert et al., 1997 |
IP | Nuclear | |||||
GST-pulldown | ||||||
GSK3-binding protein/FRAT | PDZ | Yeast two hybrid | Agonist | Cytoplasmic | GOF: canonical | Li et al., 1999; Yost et al.,1998 |
IP | LOF: canonical | |||||
Frodo | PDZ | Yeast two hybrid | Agonist | Unknown | GOF: canonical | Gloy et al., 2002 |
IP | LOF: canonical | |||||
DN: canonical | ||||||
Dapper | PDZ | Yeast two hybrid | Antagonist | Cytoplasmic | GOF: canonical | Cheyette et al., 2002 |
IP‡ | Nuclear? | LOF: canonical/PCP | ||||
GST-pulldown | DN: canonical | |||||
IDAX | PDZ | Yeast two hybrid | Antagonist | Cytoplasmic | GOF: canonical | Hino et al., 2001 |
IP | LOF: canonical | |||||
GST-pulldown | DN: canonical | |||||
Naked Cuticle | PDZ | Yeast two hybrid | Antagonist | Cytoplasmic | GOF: canonical/PCP | Rousset et al., 2001; Wharton et al., 2001 |
IP | LOF: canonical | |||||
DN: canonical | ||||||
Strabismus | PDZ | IP | Agonist/Antagonist | Membrane | GOF: PCP | Bastock et al., 2003; Jessen et al., 2002; Park and Moon, 2002; Wolff and Rubin, 1998 |
GST-pulldown | LOF: PCP | |||||
DN: PCP | ||||||
PAR1 | PDZ | Biochemical purification | Agonist/antagonist | Cytoplasmic? | PAR1BY GOF: PCP | Ossipova et al., 2005; Sun et al., 2001 |
PAR1BY LOF: PCP | ||||||
IP | PAR1A/BX GOF: canonical | |||||
PAR1A/BX LOF: canonical | ||||||
Diego/Diversin | PDZ | IP | Agonist/Antagonist | Cytoplasmic | GOF: canonical + PCP | Jenny et al., 2005 |
LOF: PCP | ||||||
Protein Phosphatase 2C | PDZ | IP | Agonist | Cytoplasmic | GOF: canonical | Ratcliffe et al., 2000; Strovel et al., 2000; Yang et al., 2003 |
Notch | PDZ | Yeast two hybrid | ND | ND | ND | Axelrod et al., 1996 |
IP | ||||||
EPS8 | PDZ | IP | Unknown | Cytoplasmic? | Unknown | Inobe et al., 1999 |
Yeast two hybrid | ||||||
Daple | PDZ | Yeast two hybrid | Agonist | Unknown | GOF: canonical + PCP | Oshita et al., 2003 |
IP | ||||||
Pulldown assay | ||||||
DAAM1 | PDZ | Yeast two hybrid | Agonist | Cytoplasmic | GOF: PCP | R.H., unpublished |
DEP | IP‡ | Membrane | LOF: PCP | |||
GST-pulldown | DN: PCP | |||||
Prickle | DEP | IP | Agonist | Cytoplasmic | GOF: PCP | Jenny et al., 2005; Tree et al., 2002 |
GST-pulldown | Membrane | LOF: PCP | ||||
DN: PCP | ||||||
MuSK | DEP | Yeast two hybrid | Agonist | Cytoplasmic | Acetylcholine receptor clustering | Luo et al., 2002 |
IP‡ | Membrane | |||||
GST-pulldown | ||||||
Disabled 2 | DEP | IP | Antagonist | Membrane | GOF: canonical | Hocevar et al., 2003 |
LOF: canonical | ||||||
EphrinB1 | Full length | IP | Agonist? | Membrane | GOF: PCP? | Tanaka et al., 2003 |
GST-pulldown | ||||||
PKCδ | Full length | IP | Agonist | Cytoplasmic | LOF: PCP | Kinoshita et al., 2003 |
Membrane | DN: PCP | |||||
NET1 | Full length | IP | Antagonist? | Cytoplasmic | GOF: PCP | Miyakoshi et al., 2004 |
β-Arrestin | Full length | IP | Unknown | Cytoplasmic | GOF: receptor internalization | Chen et al., 2003 |
AchR, acetylcholine receptor; DN, dominant negative; GOF, gain of function;GST, Gluthathione-S-Transferase-fusion protein; IDAX, Inhibitor of Dishevelled and Axin; IP, immunoprecipitation; LOF, loss of function; ND, not done;PAR1A/BX, Partitioning Defective 1A/BX; PAR1BY, Partitioning Defective 1BY.
A list of proteins that bind to Dishevelled, their region of interaction with Dishevelled and the binding assays used to detect interactions, the cellular localizations of the proteins and their function in which branch of the WNT pathway
Immunoprecipitation (IP) assays were performed using epitope-tagged protein, whereas GST-pulldown was performed using purified protein, unless stated otherwise
Assay performed using both epitope-tagged and endogenous protein