Stage . | Wild type (%) . | Bcl2l-/- (%) . | P . |
---|---|---|---|
E9.5 ov | 14.4±5.4 | 41.9±10.9 | <0.0001 |
E10.5 ed | 25.5±11.3 | 77.2±8.8 | <0.0001 |
E10.5 ov without ed | 16.9±6.5 | 29.9±5.9 | <0.0001 |
E12.5 ssd | 25.8±6.7 | 52.0±8.6 | <0.0001 |
Stage . | Wild type (%) . | Bcl2l-/- (%) . | P . |
---|---|---|---|
E9.5 ov | 14.4±5.4 | 41.9±10.9 | <0.0001 |
E10.5 ed | 25.5±11.3 | 77.2±8.8 | <0.0001 |
E10.5 ov without ed | 16.9±6.5 | 29.9±5.9 | <0.0001 |
E12.5 ssd | 25.8±6.7 | 52.0±8.6 | <0.0001 |
The proportion of TUNEL-positive cells from all otic vesicle cells was calculated on serial sections covering the whole vesicle at E9.5 or E10.5 (two embryos each genotype at each stage). In case of E10.5 the otic vesicle (ov)was divided in two areas: the endolymphatic duct (ed) and the rest of the epithelium. At E12.5, the proportion of TUNEL-positive cells was calculated in the tip of the outgrowing superior semicircular duct (ssd). The calculated mean values are given with standard deviation (±s.d.). The differences between wild-type and Bcl2l mutant values was analyzed using the two-tailed Student's t-test.