Lineage analysis of tbx-37 tbx-38 mutant embryos
| Cell name . | Cell fate . | Cell fate . | Cell fate . |
|---|---|---|---|
| Wild type | —First Notch | tbx-37 tbx-38 | |
| (—P2) | |||
| ABplp | |||
| ABplpppppp | Div< | Non div | Div< |
| ABplpppppa | Div | Non div | Div |
| ABplppppaa | Div | Non div | Div |
| ABplpppapp | Death | Non div | Death |
| —Second Notch | tbx-37 tbx-38 | ||
| (—MS) | |||
| ABalp | |||
| ABalppaaaa | Death | Hyp | Div |
| ABalppaapa | Death | Hyp | Div |
| ABalpppaap | Div | Hyp (predicted) | Div |
| ABalpppaaa | Div | Neuron (predicted) | Div |
| ABalppaaap | Div | Hyp | Div |
| ABalppappp | Div | Hyp | Div |
| ABalppppap | Div | Hyp (predicted) | Div |
| ABalpppapp | Div | Hyp (predicted) | Death |
| —Third Notch | tbx-37 tbx-38 | ||
| (—ABalapp) | |||
| ABpla | |||
| ABplaaappp | Hyp | Div | Hyp |
| ABplaaappa | Hyp | Div | Hyp |
| ABplaaaapp | Hyp | Div<; death | Hyp |
| ABplaapppp | Hyp | Hyp | Hyp |
| ABplaapppa | Hyp | Hyp | Hyp |
| —Fourth Notch | tbx-37 tbx-38 | ||
| (—MSapa/p) | |||
| ABplp | |||
| ABplpappap | Death | Div | Death |
| ABplpappaa | Div< | Div= | Div< |
| —MS, —P2 | tbx-37 tbx-38 | ||
| ABarp | |||
| (ABarppa) | |||
| ABarppappp | Hyp | Hyp | Div |
| ABarppappa | Hyp | Hyp | Div |
| ABarppaapa | Hyp | Hyp | Div |
| ABarppaaa | Div< | Div< | Div> |
| ABarppaaap | Hyp | Hyp | Death |
| (ABarppp) | |||
| ABarpppppp | Hyp | Hyp | Hyp |
| ABarpppppa | Hyp | Hyp | Hyp |
| ABarppppap | Hyp | Hyp | Hyp |
| ABarppppaa | Hyp | Hyp | Hyp |
| ABarpppapa | Hyp | Hyp | Hyp |
| ABarpppaa | Div< | Div< | Div< |
| ABarpppaap | Hyp | Hyp | Hyp |
| Cell name . | Cell fate . | Cell fate . | Cell fate . |
|---|---|---|---|
| Wild type | —First Notch | tbx-37 tbx-38 | |
| (—P2) | |||
| ABplp | |||
| ABplpppppp | Div< | Non div | Div< |
| ABplpppppa | Div | Non div | Div |
| ABplppppaa | Div | Non div | Div |
| ABplpppapp | Death | Non div | Death |
| —Second Notch | tbx-37 tbx-38 | ||
| (—MS) | |||
| ABalp | |||
| ABalppaaaa | Death | Hyp | Div |
| ABalppaapa | Death | Hyp | Div |
| ABalpppaap | Div | Hyp (predicted) | Div |
| ABalpppaaa | Div | Neuron (predicted) | Div |
| ABalppaaap | Div | Hyp | Div |
| ABalppappp | Div | Hyp | Div |
| ABalppppap | Div | Hyp (predicted) | Div |
| ABalpppapp | Div | Hyp (predicted) | Death |
| —Third Notch | tbx-37 tbx-38 | ||
| (—ABalapp) | |||
| ABpla | |||
| ABplaaappp | Hyp | Div | Hyp |
| ABplaaappa | Hyp | Div | Hyp |
| ABplaaaapp | Hyp | Div<; death | Hyp |
| ABplaapppp | Hyp | Hyp | Hyp |
| ABplaapppa | Hyp | Hyp | Hyp |
| —Fourth Notch | tbx-37 tbx-38 | ||
| (—MSapa/p) | |||
| ABplp | |||
| ABplpappap | Death | Div | Death |
| ABplpappaa | Div< | Div= | Div< |
| —MS, —P2 | tbx-37 tbx-38 | ||
| ABarp | |||
| (ABarppa) | |||
| ABarppappp | Hyp | Hyp | Div |
| ABarppappa | Hyp | Hyp | Div |
| ABarppaapa | Hyp | Hyp | Div |
| ABarppaaa | Div< | Div< | Div> |
| ABarppaaap | Hyp | Hyp | Death |
| (ABarppp) | |||
| ABarpppppp | Hyp | Hyp | Hyp |
| ABarpppppa | Hyp | Hyp | Hyp |
| ABarppppap | Hyp | Hyp | Hyp |
| ABarppppaa | Hyp | Hyp | Hyp |
| ABarpppapa | Hyp | Hyp | Hyp |
| ABarpppaa | Div< | Div< | Div< |
| ABarpppaap | Hyp | Hyp | Hyp |
Various descendants of the ABa and ABp granddaughters (left column, bold type) were examined in tbx-37 tbx-38 mutants. The wild-type fates of the cells selected for analysis are dependent on one or more of the four Notch-mediated interactions. For comparison, the fate of each cell analyzed is shown for wild-type embryos (data from Sulston et al., 1983) and shown for mutant or experimental embryos where one of the four Notch-mediated interactions does not occur [observed and predicted data from(Hutter and Schnabel, 1994; Hutter and Schnabel, 1995)]. Cell fates were determined in at least two, and usually three, embryos. Cell behaviors were scored as follows: Div (divides equally); Div< (divides unequally with smaller anterior daughter; Div> (divides unequally with larger anterior daughter); Hyp (differentiates as a hypodermal/skin cell); and Death (cell undergoes programmed cell death).