Overview of gonads analyzed
| . | Sox9flox/+ . | Sox9flox/Sox9flox . | Sox8/+; Sox9flox/+ . | Sox8/+; Sox9flox/Sox9flox . | Sox8/Sox8; Sox9flox/+ . | Sox8/Sox8; Sox9flox/Sox9flox . |
|---|---|---|---|---|---|---|
| XX | 40 | 28 | 9 | 1 | 5 | 18 |
| XY | 33 | 31 | 13 | 7 | 8 | 6 |
| XX (Sf1:Cre) | 37 | 27 | 6 | 4 | 7 | 9 |
| XY (Sf1:Cre) | 30 | 31 | 11 | 9 (4SR) | 5 | 3 (1SR) |
| . | Sox9flox/+ . | Sox9flox/Sox9flox . | Sox8/+; Sox9flox/+ . | Sox8/+; Sox9flox/Sox9flox . | Sox8/Sox8; Sox9flox/+ . | Sox8/Sox8; Sox9flox/Sox9flox . |
|---|---|---|---|---|---|---|
| XX | 40 | 28 | 9 | 1 | 5 | 18 |
| XY | 33 | 31 | 13 | 7 | 8 | 6 |
| XX (Sf1:Cre) | 37 | 27 | 6 | 4 | 7 | 9 |
| XY (Sf1:Cre) | 30 | 31 | 11 | 9 (4SR) | 5 | 3 (1SR) |
Gonads were analyzed at various stages of development and judged by the presence of sex cords (E13.5), or their external appearance and descent status(E15.5 and E18.5). Sex-reversed gonads or those with abnormal appearance were sectioned (cryostat or paraffin-wax embedded) to confirm the finding histologically. Complete sex reversal (SR) was only detected in mice homozygous for the Sox9 knock-out and heterozygous/homozygous for the Sox8 mutation. Note, table only includes gonads with complete sex reversal (no sex cords); gonads with present but abnormal sex cords were scored as testes.