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Table 1.

Overview of gonads analyzed

Sox9flox/+Sox9flox/Sox9floxSox8/+; Sox9flox/+Sox8/+; Sox9flox/Sox9floxSox8/Sox8; Sox9flox/+Sox8/Sox8; Sox9flox/Sox9flox
XX 40 28 18 
XY 33 31 13 
XX (Sf1:Cre37 27 
XY (Sf1:Cre30 31 11 9 (4SR) 3 (1SR) 
Sox9flox/+Sox9flox/Sox9floxSox8/+; Sox9flox/+Sox8/+; Sox9flox/Sox9floxSox8/Sox8; Sox9flox/+Sox8/Sox8; Sox9flox/Sox9flox
XX 40 28 18 
XY 33 31 13 
XX (Sf1:Cre37 27 
XY (Sf1:Cre30 31 11 9 (4SR) 3 (1SR) 

Gonads were analyzed at various stages of development and judged by the presence of sex cords (E13.5), or their external appearance and descent status(E15.5 and E18.5). Sex-reversed gonads or those with abnormal appearance were sectioned (cryostat or paraffin-wax embedded) to confirm the finding histologically. Complete sex reversal (SR) was only detected in mice homozygous for the Sox9 knock-out and heterozygous/homozygous for the Sox8 mutation. Note, table only includes gonads with complete sex reversal (no sex cords); gonads with present but abnormal sex cords were scored as testes.

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