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Table 2.

Possible interactions of the proteins relevant to IZ formation, revealed in a yeast two-hybrid assay

pGBKT7
pGADT7Her5HimT-antigen
Her5 
Ngn1 − 
p53 
Lam − − − 
pGBKT7
pGADT7Her5HimT-antigen
Her5 
Ngn1 − 
p53 
Lam − − − 

The assay was performed with full-length Her5, Him and Ngn1 proteins expressed from pGBKT7 and pGADT7 expression vectors. p53 expressed from pGADT7 and T-antigen expressed from pGBKT7 were used as positive controls. Lam protein from pGADT7 was used as a negative control for interactions.

+, interaction between the proteins tested, revealed by activation of ade, his and mel genes.

−, absence of interaction.

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