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Table 1.

Sequences of primers used in real-time PCR

GeneOligonucleotideSequence*Amplicon size (bp)
him forward primer 5′-GTTATATCTCCTGCGGGT-3′ 163 
 reverse primer 5′-GGGATAAGAGGAAGCCTTT-3′  
otx1 forward primer 5′-GATACCCAGCAACACCG-3′ 136 
 reverse primer 5′-ATCTTCAGTGCCACCT-3′  
pax6 forward primer 5′-TTTGCCTGGGAGATTC-3′ 167 
 reverse primer 5′-CTCTGCCCGTTGAGCATTCT-3′  
GeneOligonucleotideSequence*Amplicon size (bp)
him forward primer 5′-GTTATATCTCCTGCGGGT-3′ 163 
 reverse primer 5′-GGGATAAGAGGAAGCCTTT-3′  
otx1 forward primer 5′-GATACCCAGCAACACCG-3′ 136 
 reverse primer 5′-ATCTTCAGTGCCACCT-3′  
pax6 forward primer 5′-TTTGCCTGGGAGATTC-3′ 167 
 reverse primer 5′-CTCTGCCCGTTGAGCATTCT-3′  
*

Primers were chosen with the assistance of LightCycler Probe Design software.

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