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Table 1.

Summary of RNA-I experiments

Gene*Number of embryos injectedNumber with a specific defectInjected animals completing development (%)Animals with a specific defect (%)Specific defectWild-type function
tiptop 969 52 19.4 27.6 Fusion of distal leg, distal leg to antenna Segmentation and specification of distal leg 
Antp 418 64 39.7 38.5 Fusion of medial and distal leg segments Segmentation and specification of medial and distal leg 
Dfd 551 65 30.1 39.1 Deformed maxilla, mandible to antenna Specification of maxilla and mandible 
Scr 481 39 32.6 24.8 Labium to antenna, loss of T1 leg comb Specification of labium and leg comb 
Ubx 300 23 29.7 25.8 Ectopic appendage and sternite in A1. A1 and T3 to T2 Specification of A1 and T3 
Antp+tiptop 718 49 26.7 25.5 Fusion of medial and distal leg segments, leg to antenna  
Control 975 0 42.5 0   
Gene*Number of embryos injectedNumber with a specific defectInjected animals completing development (%)Animals with a specific defect (%)Specific defectWild-type function
tiptop 969 52 19.4 27.6 Fusion of distal leg, distal leg to antenna Segmentation and specification of distal leg 
Antp 418 64 39.7 38.5 Fusion of medial and distal leg segments Segmentation and specification of medial and distal leg 
Dfd 551 65 30.1 39.1 Deformed maxilla, mandible to antenna Specification of maxilla and mandible 
Scr 481 39 32.6 24.8 Labium to antenna, loss of T1 leg comb Specification of labium and leg comb 
Ubx 300 23 29.7 25.8 Ectopic appendage and sternite in A1. A1 and T3 to T2 Specification of A1 and T3 
Antp+tiptop 718 49 26.7 25.5 Fusion of medial and distal leg segments, leg to antenna  
Control 975 0 42.5 0   

Control refers to embryos injected with buffer and mock transcription reactions.

T1, T2, T3: first, second and third thoracic segments.

A1: first abdominal segment.

*

Gene that corresponds to the dsRNA injected

Number of embryos that produced a repeatable defect not seen in control injections of buffer

By definition, defects seen in controls, usually deletions, are not considered to be specific defects.

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