Positive signal by immunostaining or RT-PCR
| . | . | Ventral endoderm(VE)* . | VE+cardiac† . | Control tissues‡ . |
|---|---|---|---|---|
| Albumin | I | 0/8 | 19/21 | 0/10 |
| R | 0/8 | 34/35 | 0/8 | |
| Nkx2.1 | I | 0/13 | 19/32 | 0/16 |
| R | 0/10 | 28/52 | 0/14 | |
| SP-C | I | 0/6 | 12/21 | 0/9 |
| R | 0/5 | §6/19 | 0/12 | |
| CC10 | I | 0/3 | 4/9 | 0/6 |
| R | 0/5 | 6/13 | 0/2 |
| . | . | Ventral endoderm(VE)* . | VE+cardiac† . | Control tissues‡ . |
|---|---|---|---|---|
| Albumin | I | 0/8 | 19/21 | 0/10 |
| R | 0/8 | 34/35 | 0/8 | |
| Nkx2.1 | I | 0/13 | 19/32 | 0/16 |
| R | 0/10 | 28/52 | 0/14 | |
| SP-C | I | 0/6 | 12/21 | 0/9 |
| R | 0/5 | §6/19 | 0/12 | |
| CC10 | I | 0/3 | 4/9 | 0/6 |
| R | 0/5 | 6/13 | 0/2 |
Shown are the number of colonies (positive signal/total tested), as designated, from 2- to 5-somite mouse embryos that either expressed Nkx2.1,albumin, SP-C or CC10 protein (I) or mRNA (R) after 2 days of culture.
Nineteen out of 31 cultured in the presence of heparin sulfate as controls for FGF treatments.
Six out of 88 were cultured in the presence of DMSO as controls for SU5402 treatments.
Includes head and caudal regions, including those cultured in the presence of FGF1, FGF2 and FGF8b.
Four positive signals were faint.