. | . | . | . | . | . | (%) . | . | . | ||
---|---|---|---|---|---|---|---|---|---|---|
. | RNA . | Morpholino . | Dose . | n . | Normal . | Class I . | Class II . | Class III . | ||
Experiment 1 | charon | 25 pg | 86 | 0 | 5 | 72 | 23 | |||
charon | 100 pg | 106 | 0 | 1 | 18 | 81 | ||||
Experiment 2 | charon | 25 pg | 29 | 0 | 3 | 55 | 41 | |||
charon | charon-MO | 25 pg+0.8 ng | 51 | 90 | 10 | 0 | 0 |
. | . | . | . | . | . | (%) . | . | . | ||
---|---|---|---|---|---|---|---|---|---|---|
. | RNA . | Morpholino . | Dose . | n . | Normal . | Class I . | Class II . | Class III . | ||
Experiment 1 | charon | 25 pg | 86 | 0 | 5 | 72 | 23 | |||
charon | 100 pg | 106 | 0 | 1 | 18 | 81 | ||||
Experiment 2 | charon | 25 pg | 29 | 0 | 3 | 55 | 41 | |||
charon | charon-MO | 25 pg+0.8 ng | 51 | 90 | 10 | 0 | 0 |
charon RNA, or charon RNA and charon-MO were injected into one- to two-cell stage embryos and the embryos were classified into three categories (Class I-III) by morphological inspection(Fig. 4) at 10 hpf (yolk plug closure) and 24 hpf.