BrdU incorporation rates in germline and follicle cell dm2 clones
| . | Genotype . | . | |
|---|---|---|---|
| . | FRT18D/FRT18D . | dm2 18D/dm2 18D . | |
| Germline clones stage 5-8 | 28% (n=630 cells) | 10% (n=435 cells) | |
| Mosaic germline clones stage 5-8 | 31% (n=136 cells) | 6% (n=136 cells) | |
| Follicle cell clones stage 8-10 | 37% (n=410 cells) | 12% (n=449 cells) | |
| . | Genotype . | . | |
|---|---|---|---|
| . | FRT18D/FRT18D . | dm2 18D/dm2 18D . | |
| Germline clones stage 5-8 | 28% (n=630 cells) | 10% (n=435 cells) | |
| Mosaic germline clones stage 5-8 | 31% (n=136 cells) | 6% (n=136 cells) | |
| Follicle cell clones stage 8-10 | 37% (n=410 cells) | 12% (n=449 cells) | |
Ovaries containing germline or follicle cell clones were incubated with BrdU for one hour. BrdU incorporation was detected using an anti-BrdU antibody. Frequency of BrdU incorporation in wild-type clones (FRT18D) is compared with dm2 clones (dm2 FRT 18D)of approximately the same stage. Results are expressed as the percentage of BrdU-positive cells per total number of clonal cells.