Results obtained with MLC2 transgene
| Transgene construct . | Heart/total . | . | Other sites . | Fig. . |
|---|---|---|---|---|
| 5′ deletions | ||||
| 5′Δ -2990 | 15/30 | 3A | ||
| 5′Δ -1558 | 7/12 | |||
| 5′Δ -681 | 20/51 | 3C | ||
| 5′Δ -249 | 13/28 | 3D | ||
| 5′Δ -159 | 10/50 | (8)† | 3 BA, PN | 3E |
| 5′Δ -127 | 5/27* | (3)† | 12 BA. PN | 3F,G |
| 5′Δ -111 | 0/8* | 6 BA, PN | ||
| 5′Δ -85 | 0/54* | 15 BA, S | ||
| Promoter fusions | ||||
| -1558/-48(Cyt/tk)‡ | 18/98 | 4A | ||
| -249/-36 (Cyt) | 3/10 | 4B | ||
| -1558/-249(Cyt/tk)‡ | 0/5* | |||
| -681/-89 (Cyt) | 0/16* | |||
| (-123/-41)2 (Cyt) | 5/5* | 4C | ||
| (-121/-81)2 (Cyt) | 0/12* | 3 BA | 4D | |
| (-85/-42)2 (Cyt) | 0/10* | 3 spotty | ||
| Internal deletions | ||||
| Int.Δ -89/-49 | 3/45 | (1)† | 7 various | |
| Int.Δ -89/-49 + 40bp | 3/7 | |||
| Motif mutations | ||||
| PM-GATA#3 | 5/11 | |||
| PM-GATA#2 | 3/8 | 5A | ||
| PM-GATA#1 | 7/12 | |||
| PM-GATA#2,3 | 3/10* | 5B | ||
| PM-GATA#1,2 | 0/12* | 5C | ||
| CArG-like mut | 0/11* | |||
| YY1 mut | 2/6* | (0)† | 2 BA | 6B |
| CArG-like mut/YY1 mut | 3/25* | (0)† | 4 BA | 6C |
| CArG mut/YY1 mut | 12/15* | (6)† | 6 S, BA |
| Transgene construct . | Heart/total . | . | Other sites . | Fig. . |
|---|---|---|---|---|
| 5′ deletions | ||||
| 5′Δ -2990 | 15/30 | 3A | ||
| 5′Δ -1558 | 7/12 | |||
| 5′Δ -681 | 20/51 | 3C | ||
| 5′Δ -249 | 13/28 | 3D | ||
| 5′Δ -159 | 10/50 | (8)† | 3 BA, PN | 3E |
| 5′Δ -127 | 5/27* | (3)† | 12 BA. PN | 3F,G |
| 5′Δ -111 | 0/8* | 6 BA, PN | ||
| 5′Δ -85 | 0/54* | 15 BA, S | ||
| Promoter fusions | ||||
| -1558/-48(Cyt/tk)‡ | 18/98 | 4A | ||
| -249/-36 (Cyt) | 3/10 | 4B | ||
| -1558/-249(Cyt/tk)‡ | 0/5* | |||
| -681/-89 (Cyt) | 0/16* | |||
| (-123/-41)2 (Cyt) | 5/5* | 4C | ||
| (-121/-81)2 (Cyt) | 0/12* | 3 BA | 4D | |
| (-85/-42)2 (Cyt) | 0/10* | 3 spotty | ||
| Internal deletions | ||||
| Int.Δ -89/-49 | 3/45 | (1)† | 7 various | |
| Int.Δ -89/-49 + 40bp | 3/7 | |||
| Motif mutations | ||||
| PM-GATA#3 | 5/11 | |||
| PM-GATA#2 | 3/8 | 5A | ||
| PM-GATA#1 | 7/12 | |||
| PM-GATA#2,3 | 3/10* | 5B | ||
| PM-GATA#1,2 | 0/12* | 5C | ||
| CArG-like mut | 0/11* | |||
| YY1 mut | 2/6* | (0)† | 2 BA | 6B |
| CArG-like mut/YY1 mut | 3/25* | (0)† | 4 BA | 6C |
| CArG mut/YY1 mut | 12/15* | (6)† | 6 S, BA |
Cyt/tk, cytoskeletal actin or tk minimal promoters; Int.Δ, internal deletion; PM, point mutations; BA, branchial arches; PN, pronephros; S,somites.
Experiments performed with γ-crystallin GFP co-transgene. (Only embryos showing cotransgene expression were scored.)
When GFP expression was detected in other sites as well as the heart,values in parentheses indicate the number of embryos showing heart-only expression.
Promoter fragments tested with both Xenopus laevis cytoskeletal actin and herpes simplex thymidine kinase minimal promoters.