The Scl transgene partially rescues the frequency of TER119-positive cells in Vegflo yolk sacs
| Genotype . | n . | TER119+ (% total) . |
|---|---|---|
| Vegf+/+ | 4 | 77.5±6.3 |
| Vegf+/+Scltg | 8 | 79.1±11.4 |
| Vegflo/+ | 8 | 71.5±8.5* |
| Vegflo/+Scltg | 4 | 81.3±4.8† |
| Vegflo/lo | 4 | 27.0±11.1‡ |
| Vegflo/loScltg | 2 | 38.2±5.1§ |
| Genotype . | n . | TER119+ (% total) . |
|---|---|---|
| Vegf+/+ | 4 | 77.5±6.3 |
| Vegf+/+Scltg | 8 | 79.1±11.4 |
| Vegflo/+ | 8 | 71.5±8.5* |
| Vegflo/+Scltg | 4 | 81.3±4.8† |
| Vegflo/lo | 4 | 27.0±11.1‡ |
| Vegflo/loScltg | 2 | 38.2±5.1§ |
Single cell suspensions of dissected E9.0-9.5 yolk sacs were stained for TER119 and 7-AAD as in Fig. 5. The frequency of TER119-positive cells decreased according to the number of Vegflo alleles. *P<0.02 when compared with wild-type embryos, ‡P<0.001 when compared with heterozygous or wild type embryos. The Scl transgene(Scltg) fully restores the frequency of TER119-positive cells in heterozygous Vegflo/+ embryos(†P<0.001), albeit partially in homozygous Vegflo/lo littermates(§P<0.02). n represents the number of embryos of a corresponding genotype.