Activation of EGL-15 perturbs sex muscle differentiation
| Genotype† . | % Animals with differentiated sex muscles . | % Animals with mispositioned-differentiated sex muscles . | Sex muscle Pmyo-3::GFPexpression‡ . |
|---|---|---|---|
| N2 | 100 | 0 | NA |
| ccIs4251; soc-2 | 100 | 0 | + |
| soc-2; ayIs15 [egl-15(neu*)] | 0 | NA | +/– |
| soc-2 ayIs18 [5B*] | 100 | 63 | + |
| soc-2; ayIs27 [5A*] | 0 | NA | +/– |
| daf-18(lf) soc-2; ayIs15 | 100 | 100 | + |
| soc-2; pdk-1(gf) ayIs15 | 68 | 63 | + |
| soc-2; akt-1(gf); ayIs15 | 82.5 | 62 | + |
| daf-16(lf); soc-2; ayIs15 | 20 | 20 | +/– |
| Genotype† . | % Animals with differentiated sex muscles . | % Animals with mispositioned-differentiated sex muscles . | Sex muscle Pmyo-3::GFPexpression‡ . |
|---|---|---|---|
| N2 | 100 | 0 | NA |
| ccIs4251; soc-2 | 100 | 0 | + |
| soc-2; ayIs15 [egl-15(neu*)] | 0 | NA | +/– |
| soc-2 ayIs18 [5B*] | 100 | 63 | + |
| soc-2; ayIs27 [5A*] | 0 | NA | +/– |
| daf-18(lf) soc-2; ayIs15 | 100 | 100 | + |
| soc-2; pdk-1(gf) ayIs15 | 68 | 63 | + |
| soc-2; akt-1(gf); ayIs15 | 82.5 | 62 | + |
| daf-16(lf); soc-2; ayIs15 | 20 | 20 | +/– |
Forty young adult animals were scored for differentiated sex muscles after being fixed and stained with rhodamine-phalloidin.
Alleles used: soc-2(n1774), daf-18(nr2037lf), pdk-1(mg142gf),akt-1(mg144gf) and daf-16(mg54lf). ccIs4251; daf-18(lf), ccIs4251;daf-18(lf) soc-2, pdk-1(gf), akt-1(gf), daf-16(lf) and daf-16(lf);soc-2 animals all have differentiated sex muscles located in wild-type positions in 100% of the animals examined. The presence of the ccIs4251 transgene or the soc-2 mutation has no significant effects on sex muscle differentiation. The daf-18(nr2037)soc-2(n1774) double mutant has a mildly penetrant Egl phenotype and some subtle abnormalities in sex muscle morphology when stained with rhodamine-phalloidin
+, strong GFP expression in the vm1s of all animals; +/–, weak GFP expression in poorly differentiated sex muscles, some animals with no detectable GFP expression. NA, not applicable