Table 2.

Morpholino oligo phenocopy of the moz mutant phenotype

b719/b719b999/b999moz-MO1
moz-MO2
moz-MO3
5 ng15 ng5 ng15 ng5 ng15 ng
n 86 23 41 89 161 55 106 72
% with phenotype (n
Dorsal deletion 81 (70.0) 22 (5.0) 0 (0.0) 1 (1.0) 0 (0) 0 (0.0) 23 (24.0) 67 (48.5)
Dorsal shape change 100 (86.0) 100 (23.0) 52 (21.5) 86 (76.5) 17 (27.5) 92 (50.5) 93 (98.5) 98 (70.5)
Ventral inversion 99 (85.5) 54 (12.5) 12 (5.0) 29 (26.0) 0 (0.0) 0 (0.0) 44 (40.0) 75 (54.0)
Ventral shape change 100 (86.0) 72 (16.5) 35 (14.5) 60 (53.5) 3 (5.5) 37 (20.5) 86 (91.5) 95 (68.5)
Ventral fusion 98 (84.5) 33 (7.5) 15 (6.0) 19 (17.0) 0 (0.0) 2 (1.0) 41 (43.5) 69 (50.0)
Dorsal fusion 99 (85.5) 44 (10.0) 18 (7.5) 11 (9.5) 0 (0) 33 (18.0) 46 (48.5) 74 (53.5)
b719/b719b999/b999moz-MO1
moz-MO2
moz-MO3
5 ng15 ng5 ng15 ng5 ng15 ng
n 86 23 41 89 161 55 106 72
% with phenotype (n
Dorsal deletion 81 (70.0) 22 (5.0) 0 (0.0) 1 (1.0) 0 (0) 0 (0.0) 23 (24.0) 67 (48.5)
Dorsal shape change 100 (86.0) 100 (23.0) 52 (21.5) 86 (76.5) 17 (27.5) 92 (50.5) 93 (98.5) 98 (70.5)
Ventral inversion 99 (85.5) 54 (12.5) 12 (5.0) 29 (26.0) 0 (0.0) 0 (0.0) 44 (40.0) 75 (54.0)
Ventral shape change 100 (86.0) 72 (16.5) 35 (14.5) 60 (53.5) 3 (5.5) 37 (20.5) 86 (91.5) 95 (68.5)
Ventral fusion 98 (84.5) 33 (7.5) 15 (6.0) 19 (17.0) 0 (0.0) 2 (1.0) 41 (43.5) 69 (50.0)
Dorsal fusion 99 (85.5) 44 (10.0) 18 (7.5) 11 (9.5) 0 (0) 33 (18.0) 46 (48.5) 74 (53.5)

Percentage of animals with each phenotype is listed. Of 75 uninjected wild-type control animals, none had any of these six phenotypes (data not shown). The position of each MO is shown in Fig. 1A. For each class,animals were scored on both right and left sides, with each side counting as one-half of an animal. Dorsal deletion' includes animals in which no Alcian-positive cartilage articulated with the neurocranium, which in all cases included deletion of the square dorsal half (HM) of HS. dorsal shape change' class contains all phenotypes with mispatterned HSs, thus includes theHM deletion' class. Other mispatterned HSs include absent foramen, shortening of SY, or other shape changes of HM and/or SY, all of which make HS more simply triangular, resembling its first arch counterpart. Ventral inversion'contains animals in which CH, the ventral second arch cartilage, which normally forms a ∼45° angle between the lateral side of CH and the midline, makes over a 90° angle between the posterior side of CH and the midline, and branchial cartilage was not deleted. Ventral shape change'contains animals in which the second arch ventral cartilage forms an enlarged process on its lateral end (see Fig. 2). In all cases, this second arch ventral cartilage was also smaller and thinner than the wild-type condition, but the presence of the distinctive knob was the criterion for inclusion in this class. Ventral fusion' and `dorsal fusion' contain animals in which the first and second arch ventral and dorsal cartilages were visibly connected with Alcian-positive cartilage. All scoring was made on a high-power dissecting microscope in depigmented and cleared specimens. CH, ceratohyal; HM, hyomandibula; HS,hyosymplectic; SY, symplectic.

Close Modal