Primer and morpholino oligo (MO) sequences
| Primer/MO . | Sequence (5′ to 3′) . |
|---|---|
| 1 | GCCTGGCATTTTAGAAAGCGTTC |
| 2 | GAGAGCGCACCTGTACTGG |
| 3 | GCTATCTCGCGTCCTAGAAAT |
| 4 | CTGCTGAGAGGGAGACAAGTC |
| 5 | CATGATTATATTCTTGTATTTCAT |
| 6 | CGAGTGCTTGTTTACTGTAGT |
| 7 | GAGTGATGCTTCTGCACAAG |
| 8 | ACCCTTTGAAGAAGTTGTTG |
| 9 | GGGTCAGTCTTAGGCTTAAG |
| 10 | AAGGATATAAACAGCTCCAC |
| 11 | CCAGTCATCATTGACTCACC |
| 12 | ACTACCACAGTACCAGTAAAC |
| 13 | GGGAGAGGAGGAACGTAAAGAGGT |
| 14 | TGCAAACGGAAGAGGATGGTCCAGAGCTTTG |
| moz-MO1 | CATGGTTGCTTTAATACTGCTAAGC |
| moz-MO2 | tcatgttgcttacCTGCTGTACTCC |
| moz-MO3 | gagatttcctttcctcacCTCAAAC |
| hoxa2b-MO | AATTCGTAATTCATCTCCTCCAAG |
| hoxb2a-MO | ATTCAAAATTCATCGCTTCGCCTGG |
| bapx1-MO | GCGCACAGCCATGTCGAGCAGCACT |
| Primer/MO . | Sequence (5′ to 3′) . |
|---|---|
| 1 | GCCTGGCATTTTAGAAAGCGTTC |
| 2 | GAGAGCGCACCTGTACTGG |
| 3 | GCTATCTCGCGTCCTAGAAAT |
| 4 | CTGCTGAGAGGGAGACAAGTC |
| 5 | CATGATTATATTCTTGTATTTCAT |
| 6 | CGAGTGCTTGTTTACTGTAGT |
| 7 | GAGTGATGCTTCTGCACAAG |
| 8 | ACCCTTTGAAGAAGTTGTTG |
| 9 | GGGTCAGTCTTAGGCTTAAG |
| 10 | AAGGATATAAACAGCTCCAC |
| 11 | CCAGTCATCATTGACTCACC |
| 12 | ACTACCACAGTACCAGTAAAC |
| 13 | GGGAGAGGAGGAACGTAAAGAGGT |
| 14 | TGCAAACGGAAGAGGATGGTCCAGAGCTTTG |
| moz-MO1 | CATGGTTGCTTTAATACTGCTAAGC |
| moz-MO2 | tcatgttgcttacCTGCTGTACTCC |
| moz-MO3 | gagatttcctttcctcacCTCAAAC |
| hoxa2b-MO | AATTCGTAATTCATCTCCTCCAAG |
| hoxb2a-MO | ATTCAAAATTCATCGCTTCGCCTGG |
| bapx1-MO | GCGCACAGCCATGTCGAGCAGCACT |
See Materials and methods for description and number of primers. ATG translation start site is underlined for moz-MO1, both hox-MOs and bapx1-MO. For the splice-blocking moz-MOs (MO2 and 3), the intron complementary sequence is in lower case. Although the hoxa2b-MO sequence is identical to that reported in Hunter and Prince (Hunter and Prince, 2002), the hoxb2a-MO sequence we injected was three bases longer than the sequence they report.