The Company of Biologists

Table 1.

Efficiency of SCNT in Macaques^{^*}

Donor cell			Cell injection						% Reconstruced nuclear formation
Macaque	Cell type	Number of nucleated oocytes	Number of injected oocytes^A	% Success	Number of injected oocytes^{^*}	With RN	0 RN	1 RN	2 RN	≥3RN	Normal	Abnormal	One cell	Fragmented
LoTM	Cumulus	224	219	97.8	171	32.7	67.3	11.1^{^a}	14.0^{^a}	7.6	24.0	14.6^{^a}	42.7^{^a}	18.7
	Fetal skin fibroblast	54	52	96.3	44	38.6	61.4	15.9^ab	15.9^{^a}	6.8	22.7	22.7^ab	40.9^ab	13.6
LiTM	Adult skin fibroblast	259	239	92.3	171	39.8	60.2	22.8^{^b}	5.3^{^b}	11.7	24.6	34.5^{^b}	29.8^{^b}	11.1
Total		537	510	95.0	386	36.5	63.5	16.8	10.4	9.3	24.1	24.4	36.8	14.7

Donor cell			Cell injection						% Reconstruced nuclear formation
Macaque	Cell type	Number of nucleated oocytes	Number of injected oocytes^A	% Success	Number of injected oocytes^{^*}	With RN	0 RN	1 RN	2 RN	≥3RN	Normal	Abnormal	One cell	Fragmented
LoTM	Cumulus	224	219	97.8	171	32.7	67.3	11.1^{^a}	14.0^{^a}	7.6	24.0	14.6^{^a}	42.7^{^a}	18.7
	Fetal skin fibroblast	54	52	96.3	44	38.6	61.4	15.9^ab	15.9^{^a}	6.8	22.7	22.7^ab	40.9^ab	13.6
LiTM	Adult skin fibroblast	259	239	92.3	171	39.8	60.2	22.8^{^b}	5.3^{^b}	11.7	24.6	34.5^{^b}	29.8^{^b}	11.1
Total		537	510	95.0	386	36.5	63.5	16.8	10.4	9.3	24.1	24.4	36.8	14.7

RN, reconstructed nucleus.

The numbers of injected oocytes shown here are less than those given in the previous column because some were used for other experiments; for example, in PCC spindle formation checks.

Letters indicate that figures within the column show a significant difference, i.e. a is significantly different from b (P<0.05), but ab is not significantly different from a or b (P<0.05).

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