Skip to Main Content
Table 1.

Primer pairs and cycling conditions for Lightcycler real-time PCR

PCR primer pairOriginSequenceDenaturing temperatureAnnealing temperature (°C)/timeExtension temperature (°C)/timeAcquisition temperature (°C)/time
Antipodean Stennard et al., 1999  U: 5′-TGG ATT AGT TTA GGA ACA -3′ 95 55/5 72/16 83/3 
  D: 5′-CGG ATC TTA CAC TGA GGA -3′     
Antivin New U: 5′-GCC TCA AAG GGA TGT GGA G-3′ 95 55/5 72/11 81/3 
  D: 5′-TGT GGC TGA GTC AAC GAG AG-3′     
Bix1 Xanthos et. al., 2001  U: 5′-AGA GAC TCC CAG TTC ATC TGA-3′ 95 60/5 72/8 83/3 
  D: 5′-GGT AGG TGG GAA GTT GCT AAT-3′     
Bix3 Xanthos et. al., 2001  U: 5′-TCT CGC ATT CAG GTT TGG TTC C-3′ 95 60/5 72/11 83/3 
  D: 5′-ATC TCC TTG TTA GGG ATC ATA C-3′     
Bix4 Xanthos et. al., 2001  U: 5′-AGA TGC TAC AGG CTG GAG CAA-3′ 95 60/5 72/11 84/3 
  D: 5′-GTG TGT AAG GGG TGA GTC ATA-3′     
Cerberus Heasman et al., 2000 U: 5-GCT TGC AAA ACC TTG CCC TT-3′ 95 60/5 72/20 81/3 
  D: 5-CTG ATG GAA CAG AGA TCT TG-3     
Eomesodermin New U: 5′-TGG TCC TCA AGG TCA AGT CC-3′ 95 56/6 72/10 83/3 
  D: 5′-GGG GAG TTT TCA TTG CTT GA-3′     
Endodermin Sasai et al., 1996 U: 5′-TAT TCT GAC TCC TGA AGG TG-3′ 95 55/5 72/8 81/3 
  D: 5′-GAG AAC TGC CCA TGT GCC TCC-3′     
Epidermal keratin XMMR* U: 5′-CAC CAG AAC ACA GAG TAC -3′ 95 55/5 72/9 81/3 
  D: 5′-CAA CCT TCC CAT CAA CCA -3′     
eFGF New U: 5′-TAT GAA TGC AAA GGG GAA GC-3′ 95 55/5 72/10 83/3 
  D: 5′-ATA TCC GTG GCA AGA AAT GG-3′     
Fgf3 Kofron et al., 1999  U: 5′-GTC ATT TGT TTC CAG ACT TC-3′ 95 55/5 72/12 85/3 
  D: 5′-TAT CTG TAG GTG GTA CTT AG-3′     
Fgf8 Kofron et al., 1999  U: 5′-CTG GTG ACC GAC CAA CTA AG-3′ 95 55/5 72/14 86/3 
  D: 5′-ACC AGC CTT CGT ACT TGA CA-3′     
Gata2 New U: 5′-CTA AAC AGA GGA GCA AGA GC-3′ 95 55/5 72/15 86/3 
  D: 5′-CCT AAG TTC CTC AAA AC-3′     
Gata5 Xanthos et. al., 2001  U: 5′-ACC TTC AGA GCT GCG ACA CT-3′ 95 60/5 72/20 86/3 
  D: 5′-CCA ACC GGG AGC CCC GAT A-3′     
ODC Heasman et al., 2000 U: 5′-GCC ATT GTG AAG ACT CTC TCC ATT C-3′ 95 55/5 72/12 83/3 
  D: 5′-TTC GGG TGA TTC CTT GCC AC-3′     
Sprouty2 New U: 5′-TGC TCA GCC CAG GAA GTA GT-3′ 95 55/5 72/10 83/3 
  D: 5′-ACC ACA AAC AGG GCA AGA AC-3′     
Xbra Sun et al., 1999 U: 5′-TTC TGA AGG TGA GCA TGT CG-3′ 95 55/5 72/8 75/3 
  D: 5′-GTT TGA CTT TGC TAA AAG AGA CAG G-3′     
Xlim1 New U: 5′-CCCTGGCAGCAACTATGACT-3′ 95 55/5 72/11 85/3 
  D: 5′-GGTTGCCATAACCTCCATTG-3′     
Xlim5 Houston et. al., 2003 U: 5′ - CCA ACA GAC AGG CCC AAC-3′ 95 60/5 72/8 84/3 
  D: 5′-GTG GCT CCG GTG CTA CAG - 3′     
Xnr1 Kofron et al., 1999  U: 5′-TGG CCA GAT AGA GTA GAG-3′ 95 55/5 72/12 81/3 
  D: 5′-TCC AAC GGT TCT CAC TTT-3′     
Xnr5 New U: 5′-ATG AGG CCT CTG TCA ATG CT-3′ 95 55/5 72/10 80/3 
  D: 5′-GCC CTG AAT GTC TTG CAT CT-3′     
Xnot1 New U: 5′-CTGCATTTGGCCACCACCTGGC-3′ 95 55/5 72/15 86/3 
  D: 5′-GATGAGCCACACGGGTGGGTA-3′     
Xsox17 Xanthos et al., 2001  U: 5′-GCA AGA TGC TTG GCA AGT CG-3′ 95 58/5 72/8 85/3 
  D: 5′-GCT GAA GTT CTC TAG ACA CA-3′     
PCR primer pairOriginSequenceDenaturing temperatureAnnealing temperature (°C)/timeExtension temperature (°C)/timeAcquisition temperature (°C)/time
Antipodean Stennard et al., 1999  U: 5′-TGG ATT AGT TTA GGA ACA -3′ 95 55/5 72/16 83/3 
  D: 5′-CGG ATC TTA CAC TGA GGA -3′     
Antivin New U: 5′-GCC TCA AAG GGA TGT GGA G-3′ 95 55/5 72/11 81/3 
  D: 5′-TGT GGC TGA GTC AAC GAG AG-3′     
Bix1 Xanthos et. al., 2001  U: 5′-AGA GAC TCC CAG TTC ATC TGA-3′ 95 60/5 72/8 83/3 
  D: 5′-GGT AGG TGG GAA GTT GCT AAT-3′     
Bix3 Xanthos et. al., 2001  U: 5′-TCT CGC ATT CAG GTT TGG TTC C-3′ 95 60/5 72/11 83/3 
  D: 5′-ATC TCC TTG TTA GGG ATC ATA C-3′     
Bix4 Xanthos et. al., 2001  U: 5′-AGA TGC TAC AGG CTG GAG CAA-3′ 95 60/5 72/11 84/3 
  D: 5′-GTG TGT AAG GGG TGA GTC ATA-3′     
Cerberus Heasman et al., 2000 U: 5-GCT TGC AAA ACC TTG CCC TT-3′ 95 60/5 72/20 81/3 
  D: 5-CTG ATG GAA CAG AGA TCT TG-3     
Eomesodermin New U: 5′-TGG TCC TCA AGG TCA AGT CC-3′ 95 56/6 72/10 83/3 
  D: 5′-GGG GAG TTT TCA TTG CTT GA-3′     
Endodermin Sasai et al., 1996 U: 5′-TAT TCT GAC TCC TGA AGG TG-3′ 95 55/5 72/8 81/3 
  D: 5′-GAG AAC TGC CCA TGT GCC TCC-3′     
Epidermal keratin XMMR* U: 5′-CAC CAG AAC ACA GAG TAC -3′ 95 55/5 72/9 81/3 
  D: 5′-CAA CCT TCC CAT CAA CCA -3′     
eFGF New U: 5′-TAT GAA TGC AAA GGG GAA GC-3′ 95 55/5 72/10 83/3 
  D: 5′-ATA TCC GTG GCA AGA AAT GG-3′     
Fgf3 Kofron et al., 1999  U: 5′-GTC ATT TGT TTC CAG ACT TC-3′ 95 55/5 72/12 85/3 
  D: 5′-TAT CTG TAG GTG GTA CTT AG-3′     
Fgf8 Kofron et al., 1999  U: 5′-CTG GTG ACC GAC CAA CTA AG-3′ 95 55/5 72/14 86/3 
  D: 5′-ACC AGC CTT CGT ACT TGA CA-3′     
Gata2 New U: 5′-CTA AAC AGA GGA GCA AGA GC-3′ 95 55/5 72/15 86/3 
  D: 5′-CCT AAG TTC CTC AAA AC-3′     
Gata5 Xanthos et. al., 2001  U: 5′-ACC TTC AGA GCT GCG ACA CT-3′ 95 60/5 72/20 86/3 
  D: 5′-CCA ACC GGG AGC CCC GAT A-3′     
ODC Heasman et al., 2000 U: 5′-GCC ATT GTG AAG ACT CTC TCC ATT C-3′ 95 55/5 72/12 83/3 
  D: 5′-TTC GGG TGA TTC CTT GCC AC-3′     
Sprouty2 New U: 5′-TGC TCA GCC CAG GAA GTA GT-3′ 95 55/5 72/10 83/3 
  D: 5′-ACC ACA AAC AGG GCA AGA AC-3′     
Xbra Sun et al., 1999 U: 5′-TTC TGA AGG TGA GCA TGT CG-3′ 95 55/5 72/8 75/3 
  D: 5′-GTT TGA CTT TGC TAA AAG AGA CAG G-3′     
Xlim1 New U: 5′-CCCTGGCAGCAACTATGACT-3′ 95 55/5 72/11 85/3 
  D: 5′-GGTTGCCATAACCTCCATTG-3′     
Xlim5 Houston et. al., 2003 U: 5′ - CCA ACA GAC AGG CCC AAC-3′ 95 60/5 72/8 84/3 
  D: 5′-GTG GCT CCG GTG CTA CAG - 3′     
Xnr1 Kofron et al., 1999  U: 5′-TGG CCA GAT AGA GTA GAG-3′ 95 55/5 72/12 81/3 
  D: 5′-TCC AAC GGT TCT CAC TTT-3′     
Xnr5 New U: 5′-ATG AGG CCT CTG TCA ATG CT-3′ 95 55/5 72/10 80/3 
  D: 5′-GCC CTG AAT GTC TTG CAT CT-3′     
Xnot1 New U: 5′-CTGCATTTGGCCACCACCTGGC-3′ 95 55/5 72/15 86/3 
  D: 5′-GATGAGCCACACGGGTGGGTA-3′     
Xsox17 Xanthos et al., 2001  U: 5′-GCA AGA TGC TTG GCA AGT CG-3′ 95 58/5 72/8 85/3 
  D: 5′-GCT GAA GTT CTC TAG ACA CA-3′     
*

Xenopus Molecular Marker Resource(http://www.xenbase.org/xmmr/Marker_pages/primers.html).

View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.
Accept