The Company of Biologists

Table 1.

Limb phenotypes of Tg-Hoxd12;Gli3^+/−embryos

	Forelimb digits			Hindlimb digits
Embryo	Left	Right	Left	Right	Tibia shortened
1	Xt-like	1,2^{^*} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5	1,2^{^*} ,2,3,4,5	+
2	Xt-like	Xt-like	Xt-like	2^{^*} ,1,2,3,4,5	+
3	1,2^{^*} ,2,3,4,5	Xt-like	2^{^*} ,1,2,3,4,5	2^{^*} ,1,2,3,4,5	+
4	Xt-like	1,2^{^*} ,2,3,4,5	Xt-like	2^{^*} ,1,2,3,4,5	+
5	Xt-like	Xt-like	Xt-like	Xt-like
6	Xt-like	Xt-like	2^{^*} ,1,2,3,4,5	2^{^*} ,1,2,3,4,5	+
7	Xt-like	Xt-like	Xt-like	Xt-like
8	Xt-like	1,2^{^*} ,2,3,4,5	2^{^*} ,1,2,3,4,5	2^{^},2^{^} ,1,2,3,4,5	+
9	Xt-like	Xt-like	Xt-like	2^{^*} ,1,2,3,4,5
10	1,2^{^*} ,2,3,4,5	Xt-like	2^{^},2^{^} ,1,2,3,4,5	1,2^{^*} ,2,3,4,5
11	Xt-like	1,2^{^*} ,2,3,4,5	2^{^*} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5
12	1,2^{^*} ,2,3,4,5	Xt-like	2^{^*} ,2,3,4,5	2^{^*} ,1,2,3,4,5	+
13	Xt-like	1,1,1,2,3,4,5	2^{^},2^{^} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5	+
14	Xt-like	Xt-like	2^{^},2^{^} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5	+
15	Xt-like	1,1,1,2,3,4,5	Xt-like	2^{^*} ,1,2,3,4,5
16	Xt-like	1,1,2^{^*} ,2,3,4,5	2^{^*} ,1,2,3,4,5	1,2^{^*} ,2,3,4,5
17	Xt-like	Xt-like	2^{^},2^{^} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5
18	Xt-like	Xt-like	2^{^},2^{^} ,1,2,3,4,5	2^{^},2^{^} ,1,2,3,4,5	+
19	1,2^{^*} ,2,3,4,5	Xt-like	2^{^*} ,1,2,3,4,5	1,1,1,2,3,4,5
20	1,2^{^*} ,2,3,4,5	Xt-like	Xt-like	Xt-like
21	Xt-like	1,2^{^*} ,2,3,4,5	Xt-like	2^{^},2^{^} ,1,2,3,4,5	+
22	Xt-like	Xt-like	2^{^},2^{^} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5	+
23	Xt-like	1,2^{^*} ,2,3,4,5	2^{^*} ,1,2,3,4,5	1,2^{^*} ,1,2,3,4,5	+
24	Xt-like	1,2^{^*} ,2,3,4,5	2^{^},2^{^} ,1,2,3,4,5	2^{^*} ,1,1,2,3,4,5	+
25	Xt-like	Xt-like	1,1,1,2,3,4,5	Xt-like
26	Xt-like	Xt-like	Xt-like	Xt-like
Total Xt-like	11			4		NA
Total more severe	15			22		14

	Forelimb digits			Hindlimb digits
Embryo	Left	Right	Left	Right	Tibia shortened
1	Xt-like	1,2^{^*} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5	1,2^{^*} ,2,3,4,5	+
2	Xt-like	Xt-like	Xt-like	2^{^*} ,1,2,3,4,5	+
3	1,2^{^*} ,2,3,4,5	Xt-like	2^{^*} ,1,2,3,4,5	2^{^*} ,1,2,3,4,5	+
4	Xt-like	1,2^{^*} ,2,3,4,5	Xt-like	2^{^*} ,1,2,3,4,5	+
5	Xt-like	Xt-like	Xt-like	Xt-like
6	Xt-like	Xt-like	2^{^*} ,1,2,3,4,5	2^{^*} ,1,2,3,4,5	+
7	Xt-like	Xt-like	Xt-like	Xt-like
8	Xt-like	1,2^{^*} ,2,3,4,5	2^{^*} ,1,2,3,4,5	2^{^},2^{^} ,1,2,3,4,5	+
9	Xt-like	Xt-like	Xt-like	2^{^*} ,1,2,3,4,5
10	1,2^{^*} ,2,3,4,5	Xt-like	2^{^},2^{^} ,1,2,3,4,5	1,2^{^*} ,2,3,4,5
11	Xt-like	1,2^{^*} ,2,3,4,5	2^{^*} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5
12	1,2^{^*} ,2,3,4,5	Xt-like	2^{^*} ,2,3,4,5	2^{^*} ,1,2,3,4,5	+
13	Xt-like	1,1,1,2,3,4,5	2^{^},2^{^} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5	+
14	Xt-like	Xt-like	2^{^},2^{^} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5	+
15	Xt-like	1,1,1,2,3,4,5	Xt-like	2^{^*} ,1,2,3,4,5
16	Xt-like	1,1,2^{^*} ,2,3,4,5	2^{^*} ,1,2,3,4,5	1,2^{^*} ,2,3,4,5
17	Xt-like	Xt-like	2^{^},2^{^} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5
18	Xt-like	Xt-like	2^{^},2^{^} ,1,2,3,4,5	2^{^},2^{^} ,1,2,3,4,5	+
19	1,2^{^*} ,2,3,4,5	Xt-like	2^{^*} ,1,2,3,4,5	1,1,1,2,3,4,5
20	1,2^{^*} ,2,3,4,5	Xt-like	Xt-like	Xt-like
21	Xt-like	1,2^{^*} ,2,3,4,5	Xt-like	2^{^},2^{^} ,1,2,3,4,5	+
22	Xt-like	Xt-like	2^{^},2^{^} ,2,3,4,5	2^{^},2^{^} ,2,3,4,5	+
23	Xt-like	1,2^{^*} ,2,3,4,5	2^{^*} ,1,2,3,4,5	1,2^{^*} ,1,2,3,4,5	+
24	Xt-like	1,2^{^*} ,2,3,4,5	2^{^},2^{^} ,1,2,3,4,5	2^{^*} ,1,1,2,3,4,5	+
25	Xt-like	Xt-like	1,1,1,2,3,4,5	Xt-like
26	Xt-like	Xt-like	Xt-like	Xt-like
Total Xt-like	11			4		NA
Total more severe	15			22		14

Xt-like, single extra anterior digit 1 indistinguishable from that observed with the hemizygous Gli3^+/−Xt^Jallele.

NA, not applicable (tibia of wild type and Gli3^+/− are indistinguishable).

Mouse digits 2-4 are all triphalangeal, of a similar size, and consequently are difficult to distinguish. Therefore, additional polydactylous digits have arbitrarily been designated as digit 2^* (when they are triphalangeal) to distinguish them from the biphalangeal digit 1 duplications typical of Gli3^+/− embryos.

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