Table 2.

Arnt–/– rescue

CulturesG418Trans-CFCBL-CFC(%) BL/(BL+Trans)
Arnt+/+ 
Arnt+/+ 76.0 (±40.4) 253 (±30.8) 76 
Arnt-/- 257 (±95.8) 182 (±48.0) 41 
Arnt+/+:Arnt-/-* 134 (±10.6) 580(±45.2) 81 
Arnt+/+:Arnt-/-* 58 (±4.8) 264 (±50.8) 82 
CulturesG418Trans-CFCBL-CFC(%) BL/(BL+Trans)
Arnt+/+ 
Arnt+/+ 76.0 (±40.4) 253 (±30.8) 76 
Arnt-/- 257 (±95.8) 182 (±48.0) 41 
Arnt+/+:Arnt-/-* 134 (±10.6) 580(±45.2) 81 
Arnt+/+:Arnt-/-* 58 (±4.8) 264 (±50.8) 82 

5 × 104 cells were replated in methylcellulose.

Colonies, transitional (Trans-CFC) and hemangioblast (BL-CFC), were counted from triplicate plates and expressed either as an average (±s.d.) or as percentages.

*

1 × 105 cells were replated in these cases.

Selection with 0.4 mg/ml of G418.

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