. | dsRNA* . | Concentration (mg/ml) . | Non-specific n(%)†,§ . | Wild type n (%) . | Class I n(%)†,‡ . | Class II n(%)†,‡ . | Class III n(%)†,‡ . | Totals n . |
---|---|---|---|---|---|---|---|---|
Kr pRNAi | 5′ | 2.0 | 56 (27.5) | 0 (0) | 0 (0) | 103 (50.5) | 45 (22.1) | 204 |
3′ small | 2.0 | 7 (8.5) | 0 (0) | 0 (0) | 0 (0) | 75 (91.5) | 82 | |
3′ large | 2.0 | 31 (11.7) | 0 (0) | 0 (0) | 36 (13.6) | 198 (74.7) | 265 | |
3′ large | 0.02 | 73 (12.9) | 0 (0) | 12 (2.1) | 268 (47.5) | 211 (37.4) | 564 | |
3′ large | 0.004 | 35 (12.1) | 101 (34.8) | 44 (15.2) | 95 (32.8) | 15 (5.2) | 290 | |
Kr pRNAi Totals | 202 (14.4) | 101 (7.2) | 56 (4.0) | 502 (35.7) | 544 (38.7) | 1405 | ||
Kr eRNAi | 3′ large | 2.0 | 30 (19.0) | 3 (1.9) | 0 (0) | 8 (5.1) | 117 (74.1) | 158 |
Buffer pRNAi | 14 (6.8) | 191 (93.2) | 0 (0) | 0 (0) | 0 (0) | 205 |
. | dsRNA* . | Concentration (mg/ml) . | Non-specific n(%)†,§ . | Wild type n (%) . | Class I n(%)†,‡ . | Class II n(%)†,‡ . | Class III n(%)†,‡ . | Totals n . |
---|---|---|---|---|---|---|---|---|
Kr pRNAi | 5′ | 2.0 | 56 (27.5) | 0 (0) | 0 (0) | 103 (50.5) | 45 (22.1) | 204 |
3′ small | 2.0 | 7 (8.5) | 0 (0) | 0 (0) | 0 (0) | 75 (91.5) | 82 | |
3′ large | 2.0 | 31 (11.7) | 0 (0) | 0 (0) | 36 (13.6) | 198 (74.7) | 265 | |
3′ large | 0.02 | 73 (12.9) | 0 (0) | 12 (2.1) | 268 (47.5) | 211 (37.4) | 564 | |
3′ large | 0.004 | 35 (12.1) | 101 (34.8) | 44 (15.2) | 95 (32.8) | 15 (5.2) | 290 | |
Kr pRNAi Totals | 202 (14.4) | 101 (7.2) | 56 (4.0) | 502 (35.7) | 544 (38.7) | 1405 | ||
Kr eRNAi | 3′ large | 2.0 | 30 (19.0) | 3 (1.9) | 0 (0) | 8 (5.1) | 117 (74.1) | 158 |
Buffer pRNAi | 14 (6.8) | 191 (93.2) | 0 (0) | 0 (0) | 0 (0) | 205 |
The 5′, 3′ small, or 3′ large dsRNAs refer to different fragments of the Krüppel gene product and correspond to different regions of the transcript. See Fig. 1.
Percentages may not add up to 100 because of rounding.
Phenotypic classification was determined based on severity of phenotype,with class III being the most severe.
The non-specific category includes embryos where some embryonic development had occurred, but was uninterpretable.