Skip to Main Content
Table 1.

Distribution of WGA-Au-labelled cells in splotch embryos after orthotopic grafting*

Number of embryos (%) showing labelled cells along the migratory pathways
Genotype of donor tissueGenotype of recipient embryosNumber of recipients examinedMesenchymal region dorsolateral to the neural tubeMedial pathwayPeri-aortic mesenchymeMesenchymal region lateral to the pharynx
+/+ +/+ 6 (100%) 6 (100%) 6 (100%) 6 (100%) 
 Sp2H/+ 16 16 (100%) 16 (100%) 16 (100%) 16 (100%) 
 Sp2H/Sp2H 7 (100%) 7 (100%) 7 (100%) 5 (71%) 
Sp2H/+ +/+ 10 10 (100%) 8 (80%) 10 (100%) 8 (80%) 
 Sp2H/+ 24 24 (100%) 16 (67%) 24 (100%) 12 (50%) 
 Sp2H/Sp2H 10 2 (20%) 2 (20%) 2 (20%) 1 (10%) 
Sp2H/Sp2H +/+ 7 (100%) 7 (100%) 7 (100%) 7 (100%) 
 Sp2H/+ 3 (100%) 3 (100%) 3 (100%) 0 
 Sp2H/Sp2H 2 (25%) 1 (13%) 1 (13%) 1 (13%) 
Number of embryos (%) showing labelled cells along the migratory pathways
Genotype of donor tissueGenotype of recipient embryosNumber of recipients examinedMesenchymal region dorsolateral to the neural tubeMedial pathwayPeri-aortic mesenchymeMesenchymal region lateral to the pharynx
+/+ +/+ 6 (100%) 6 (100%) 6 (100%) 6 (100%) 
 Sp2H/+ 16 16 (100%) 16 (100%) 16 (100%) 16 (100%) 
 Sp2H/Sp2H 7 (100%) 7 (100%) 7 (100%) 5 (71%) 
Sp2H/+ +/+ 10 10 (100%) 8 (80%) 10 (100%) 8 (80%) 
 Sp2H/+ 24 24 (100%) 16 (67%) 24 (100%) 12 (50%) 
 Sp2H/Sp2H 10 2 (20%) 2 (20%) 2 (20%) 1 (10%) 
Sp2H/Sp2H +/+ 7 (100%) 7 (100%) 7 (100%) 7 (100%) 
 Sp2H/+ 3 (100%) 3 (100%) 3 (100%) 0 
 Sp2H/Sp2H 2 (25%) 1 (13%) 1 (13%) 1 (13%) 
*

Splotch embryos with 5 to 6 somites were orthotopically grafted with a WGA-Au-labelled neural crest fragment at the S2 level and were cultured in vitro for 24 hours. Embryos with 20 to 21 somites were examined for the distribution of labelled cells after sectioning

P<0.05, significantly different from the value for +/+ →+/+ grafting by Fisher's exact test

Close Modal

or Create an Account

Close Modal
Close Modal