Table 1.

Summary of silencing assays

lacI repeat linelacI repeat locationCopies of lacI repeatsEye color phenotype without lacI-HP1Eye color phenotype with lacI-HP1P-element insertion site
157.1 1E4 256 Orange White None 
179.1 86D 256 Orange White 8.63 kb downstream of Sodh-2 
157.4.112 61F3 256 Orange White 17 bp upstream of BtbII
P6.4 88E12-F1 256 Yellow White 1.69 kb downstream of ea 
P20.21 99A4-5 256 Orange White None 
P8.6 96B1 256 Orange White 25 bp upstream of BcDNA:GH12663 
P11.3 96C1-2 256 Yellow White None 
P19.9 63C5 256 Orange White None 
P2.5 65D4-5 256 Orange White 6 bp upstream of sgl 
P23.9 87D9-11 256 Yellow White 4 bp downstream of CtBP 
J11.1 42C2-3 256 Yellow White 444 bp upstream of l(2) 01289 
J3.2 93A7-B1 256 Red Red 62 bp upstream of Atpα 
F3.1.1 54B17-C1 256 Orange White None 
107.1 40B-D 256 Orange White None 
P25.22 89B2-B3 256 Orange White 3.45 kb upstream of He189B 
J8.1 89B16-17 ∼58 Orange White 6.5 kb upstream of tara 
S4.5 73A1-73A2 ∼58 Dark orange Yellow None 
S11.7 61D1 ∼55 Dark orange Yellow None 
S9.2 73A2-73A4 ∼46 Red Yellow 48 bp downstream of argos 
S15.14 1E4 ∼33 Orange Yellow None 
CN2 50C20-22 32 Orange Light orange 3.84 kb upstream of Aats-phe 
CN10 63C5 32 Dark orange Yellow None 
P9.4 85A7-9 ∼20 Dark orange Yellow None 
J2.3 86E13-15 ∼15 Orange Light orange None 
6.4P5 77B6-9 Dark orange Orange 166 bp upstream of trb1 
64.AB 67A8-B1 Orange Light orange None 
lacI repeat linelacI repeat locationCopies of lacI repeatsEye color phenotype without lacI-HP1Eye color phenotype with lacI-HP1P-element insertion site
157.1 1E4 256 Orange White None 
179.1 86D 256 Orange White 8.63 kb downstream of Sodh-2 
157.4.112 61F3 256 Orange White 17 bp upstream of BtbII
P6.4 88E12-F1 256 Yellow White 1.69 kb downstream of ea 
P20.21 99A4-5 256 Orange White None 
P8.6 96B1 256 Orange White 25 bp upstream of BcDNA:GH12663 
P11.3 96C1-2 256 Yellow White None 
P19.9 63C5 256 Orange White None 
P2.5 65D4-5 256 Orange White 6 bp upstream of sgl 
P23.9 87D9-11 256 Yellow White 4 bp downstream of CtBP 
J11.1 42C2-3 256 Yellow White 444 bp upstream of l(2) 01289 
J3.2 93A7-B1 256 Red Red 62 bp upstream of Atpα 
F3.1.1 54B17-C1 256 Orange White None 
107.1 40B-D 256 Orange White None 
P25.22 89B2-B3 256 Orange White 3.45 kb upstream of He189B 
J8.1 89B16-17 ∼58 Orange White 6.5 kb upstream of tara 
S4.5 73A1-73A2 ∼58 Dark orange Yellow None 
S11.7 61D1 ∼55 Dark orange Yellow None 
S9.2 73A2-73A4 ∼46 Red Yellow 48 bp downstream of argos 
S15.14 1E4 ∼33 Orange Yellow None 
CN2 50C20-22 32 Orange Light orange 3.84 kb upstream of Aats-phe 
CN10 63C5 32 Dark orange Yellow None 
P9.4 85A7-9 ∼20 Dark orange Yellow None 
J2.3 86E13-15 ∼15 Orange Light orange None 
6.4P5 77B6-9 Dark orange Orange 166 bp upstream of trb1 
64.AB 67A8-B1 Orange Light orange None 

Nearby genes listed are only those in which the transcription start site,or a predicted transcription start site, resides within 10 kb of the P-element insert according to FlyBase. `None' indicates absence of genes within 10 kb of the P-element insert site. `Upstream' and `downstream' denote the location of the P-element with respect to the gene. `∼' indicates lines in which the number of lac repeats were estimated based on the size of thelac repeat-containing fragment.

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