Table 2.

Summary of aCC phenotypes

aCC axons (%)
Rescued withnWild type (posterior extension)Posterior extension, truncatedAnterior extensionCrossed midlineNo axonsNot visible
None 1 275 34 19 12 33 
None 2 103 33 16 18 33 60 
Wild-type Eve 261 100 
t-wild-type Eve 169 93 
t-EveH 128 31 17 43 46 
t-EveNH 132 21 13 63 35 
EveΔC 176 48 33 12 
t-EveΔR 221 48 34 11 
t-EveH-En 192 95 
t-Tc-Eve 208 97 
t-Sa-Eve 157 100 
t-Evx1 157 71 
aCC axons (%)
Rescued withnWild type (posterior extension)Posterior extension, truncatedAnterior extensionCrossed midlineNo axonsNot visible
None 1 275 34 19 12 33 
None 2 103 33 16 18 33 60 
Wild-type Eve 261 100 
t-wild-type Eve 169 93 
t-EveH 128 31 17 43 46 
t-EveNH 132 21 13 63 35 
EveΔC 176 48 33 12 
t-EveΔR 221 48 34 11 
t-EveH-En 192 95 
t-Tc-Eve 208 97 
t-Sa-Eve 157 100 
t-Evx1 157 71 

The first two columns are as described in Table 1. Columns 3-7 show the percentage of neurons with the following phenotypes. Wild type indicates extended an axon posterolaterally, which turned at the aISN, and clearly extended further; however, in the unrescued lines, even the few in this category did not extend fully to the dorsal muscle field (see Fig. 5). `Posterior extension,truncated' indicates extended an axon posteriorly, including short axons which contacted a nearby RP2 axon. `Anterior extension' indicates extended an axon anteriorly, including short axons which contacted a nearby RP2 axon. `Crossed midline' indicates axon crossed the midline (midline crossing did not occur in any of the other categories); the majority (>90%) of these crossed at the posterior commissure. `No axons' indicates no visible axons. Owing to the necessity of using only one copy of the marker in some lines, the intensity of staining was reduced, especially that of aCC. In cases where pCC neurons were visible but not the corresponding aCC, they were counted as not visible, and were not included in the n (second column).

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