Gene(alleles)† . | DT length‡ . | TC length‡ . | DT diameter defects . | Diameter defects in other primary branches . | 2A12 staining defects§ . | AS55 staining defects§ . | Missing lumen in ganglionic branches§ . | Onset of phenotypes . |
---|---|---|---|---|---|---|---|---|
nrv2 (nwu3) | 113±2% | 99±3% | ++ | ++ | + | + | + | Stage 15 |
(23B) | 126±2% | 109±6% | ++ | ++ | + | + | + | Stage 15 |
coracle (4, 5, 4/5) | 122±2% | 104±3% | ++ | ++ | + | + | + | Stage 15 |
neurexin IV (4865, 4025) | 116±4% | N/D | ++ | ++ | ++ | ++ | + | Stage 15 |
neuroglian (14, 17) | 121±2% | 94±3% | ++ | ++ | + | + | + | Stage 15 |
gliotactin (J29-41bl, AE2-4) | 146±6% | N/D | + | + | +/- | N/D | - | Stage 15 |
Gene(alleles)† . | DT length‡ . | TC length‡ . | DT diameter defects . | Diameter defects in other primary branches . | 2A12 staining defects§ . | AS55 staining defects§ . | Missing lumen in ganglionic branches§ . | Onset of phenotypes . |
---|---|---|---|---|---|---|---|---|
nrv2 (nwu3) | 113±2% | 99±3% | ++ | ++ | + | + | + | Stage 15 |
(23B) | 126±2% | 109±6% | ++ | ++ | + | + | + | Stage 15 |
coracle (4, 5, 4/5) | 122±2% | 104±3% | ++ | ++ | + | + | + | Stage 15 |
neurexin IV (4865, 4025) | 116±4% | N/D | ++ | ++ | ++ | ++ | + | Stage 15 |
neuroglian (14, 17) | 121±2% | 94±3% | ++ | ++ | + | + | + | Stage 15 |
gliotactin (J29-41bl, AE2-4) | 146±6% | N/D | + | + | +/- | N/D | - | Stage 15 |
Phenotypes of stage 16 embryos. For all mutants, early tracheal development, including primary branch budding and outgrowth, lumen formation and morphology, and TL1 antigen expression occurred normally during stages 11-14. In all cases, tracheal branches fused at the correct stages to form the contiguous tubular networks. -, normal; +, mild; ++, moderate; +++, severe,N/D, not determined.
Where multiple alleles are listed, all alleles of each gene had comparable tracheal phenotypes.
Values shown (mean±s.e.m., n>5) have been normalized to stage 16 wild-type values (embryo length, 361±4 μm; DT length,194±1 μm; TC length, 47±1 μm) as described by Beitel and Krasnow (Beitel and Krasnow,2000). Values different from wild type (P<0.005) are in bold. Measurements were made on cor[4]/cor[5], nrx[4865]/nrx[4025],nrg[17], and gli[J29-41bl] embryos. Average lengths of embryos were nrv2[nwu3], 349±6 μm; nrv2[23B], 365±10 μm; cor, 372±4μm; nrx, 375±6 μm; nrg, 330±3 μm; gli, 377±19 μm.
Defects included non-staining regions of ganglionic branches and/or delayed or reduced staining in the dorsal or other branches.